Extractive of compound formula of ophiopogon decoction and caper euphorbia seed and reed stem soup and application thereof in preparing medicament for inhibiting H460 cell proliferation

The technology of Ophiopogon japonicus and extract is applied in the application field of preparing drugs for inhibiting the proliferation of H460 cells, and can solve the problems of unsatisfactory effect of radiotherapy, poor curative effect and high drug resistance.

Inactive Publication Date: 2010-02-17
NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] Advances in the treatment of lung cancer with Western medicine: Non-small cell lung cancer (NSCLC) accounts for more than 80% of lung cancer. In countries and cities, it is the first of various cancers, 65% of which are stage III and IV when they are discovered, often without surgical conditions, and the effect of radiotherapy is not satisfactory. Although chemotherapy is a systemic drug in drug treatment, in In the past 10 years, great progress has been made compared with the past. The emergence of a large numbe...

Method used

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  • Extractive of compound formula of ophiopogon decoction and caper euphorbia seed and reed stem soup and application thereof in preparing medicament for inhibiting H460 cell proliferation
  • Extractive of compound formula of ophiopogon decoction and caper euphorbia seed and reed stem soup and application thereof in preparing medicament for inhibiting H460 cell proliferation
  • Extractive of compound formula of ophiopogon decoction and caper euphorbia seed and reed stem soup and application thereof in preparing medicament for inhibiting H460 cell proliferation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Example 1: Materials and methods.

[0049] 1. Main reagents

[0050] Rotary evaporator (Nanjing Jinzheng Teaching Instrument Co., Ltd., 1002 lifting constant temperature water bath), water circulation vacuum pump (Yingyu Yuhua Instrument Factory, Gongyi City, Henan Province), digital display thermostat heating mantle (Zhangzhishan Town, Tongzhou City), HH-S Digital display constant temperature water bath (Jintan Medical Instrument Factory), round bottom flask (20L), condenser tube, iron stand, separatory funnel (5000mL) (Nanjing Jinzheng Teaching Instrument Co., Ltd.).

[0051] Ophiopogon japonicus (Sichuan), French pinellia (Guizhou), sun-dried ginseng (Jilin), licorice (Inner Mongolia), reed stem (Anhui), coix seed (Guizhou), winter melon seeds (Anhui), peach kernel (Shandong).

[0052] Medicinal ethanol (analytical grade, Nanjing Chemical Reagent Co., Ltd.), cyclohexane (analytical grade, Shanghai Shisi Hewei Chemical Co., Ltd.), ethyl acetate (analytical grade, Nan...

Embodiment 2

[0056] Example 2: Preparation of the extract of Maimendong decoction combined with Qianjinweijing decoction.

[0057]Weigh 200g of Ophiopogon japonicus, 100g of Pinellia chinensis, 200g of raw sun-dried ginseng, 80g of licorice, 200g of reed stem, 200g of coix seed, 200g of winter melon seed, 100g of peach kernel, and make up a total of 1280g of the whole formula, crush it and put it in a 20,000mL round bottom flask , add 10 times the amount of medicinal materials in pure water, shake and mix, soak for 30 minutes; connect the reflux condensing device, put it in the electric heating mantle and heat slowly to 110°C to boil, and keep boiling slightly for 2 hours, stop heating, and place it to the solution temperature Cool down to room temperature, remove the reflux condenser, pour out the solution in the flask, and filter it with gauze. Add purified water to the dregs to the same scale as the first time, extract once more in the same way, combine the extracts, and concentrate und...

Embodiment 3

[0058] Example 3: Experiment of the inhibitory effect of the ethyl acetate extraction fraction (MW-06 fraction) on H460 cells.

[0059] 1. Experimental method

[0060] 1.1 Cell proliferation inhibition test (MTT) method

[0061] Take the NCI-H460 cells and HFL-1 cells in the logarithmic growth phase, and adjust the cell concentration to 1×10 4 / mL, inoculated in a 96-well culture plate, 200 μL per well, cultured for 24 hours, aspirated the supernatant, and replaced each well with 200 μL of serum-free medium. After 12 hours, aspirated the culture solution. Add 200 uL of 0.1 μg / mL, 0.5 μg / mL, 1 μg / mL, 5 μg / mL, 10 μg / mL, 50 μg / mL, 100 μg / mL drug-containing culture solution into each well, and add an equal volume of DMSO (final concentration 1‰) culture medium, the positive control group was added with a final concentration of 1 μg / mL equal volume of cisplatin injection (DDP). Set 6 parallel wells in each group. After 72 hours of treatment, add 5mg / mL MTT20uL to each well, and ...

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Abstract

The invention discloses an extractive of a compound formula of ophiopogon decoction and caper euphorbia seed and reed stem soup. The extractive is thick paste which is obtained by treating a compoundformula of ophiopogon decoction and caper euphorbia seed and reed stem soup by a series of steps of water extraction, alcohol precipitation and extraction of cyclohexane and ethylacetate. The invention discloses a preparation method of the extractive and also discloses the application of the extractive in preparing a medicament for inhibiting H460 cell proliferation. The extractive of a compound formula of ophiopogon decoction and caper euphorbia seed and reed stem soup is characterized in that the extraction of the ethylacetate can obviously inhibit the growth of human lung giant cell carcinoma H460 and has no obvious influences on human normal cells, and the extraction has the effect of cell mediated cytotoxicity and can further separate and screen components and monomeric compounds forinhibiting the proliferation of the human lung giant cell carcinoma H460.

Description

technical field [0001] The invention relates to a traditional Chinese medicine extract and its application, in particular to the extract of Maimendong decoction combined with Qianjin Weijing decoction and its application in the preparation of medicines for inhibiting H460 cell proliferation. Background technique [0002] Lung cancer is one of the common malignant tumors in all countries in the world. It is the fastest growing among all malignant tumors. It is the main cause of human cancer death and is considered to be the most harmful malignant tumor to human health and life in the world today. About 3 million new cases are added every year in the world, and at the same time, more than 1 million people die of lung cancer every year. In my country, lung cancer accounts for the first place in the incidence and death of malignant tumors, with an annual incidence of 35 cases per 100,000 people and a 5-year survival rate of only 10%. Male lung cancer ranks first, while China ran...

Claims

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Application Information

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IPC IPC(8): A61K36/8994A61P35/00
Inventor 张旭唐于平郑璐玉戴晓明蒋明马健段金廒詹瑧周宇辉孙怡蒋凤荣赵宏宇姜淼汤琳熊飞
Owner NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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