Kit for genotyping VZV, production method of kit and application of kit

Abstract

The invention provides a kit for genotyping VZV (Varicella-Zoster Viruses). The kit is characterized by comprising a nucleotide sequence shown as the Table 3 in the specification, and specific primers and specific probes corresponding to clade1-5 type VZV of chemical structures. The kit has the function of detecting various kinds of VZV DNA (Deoxyribonucleic Acid); the detection sensitivity is 10<2> copies / reaction; no cross reaction with human genome, herpes simplex viruses type I / type II, cytomegaloviruses and EB viruses exists; and the kit is applicable to the virus gene diagnosis of clinical VZV infected persons, and can also be used for the epidemiology survey of different Clade types of VZV.

Description

technical field [0001] The invention belongs to the technical field of microbial gene diagnosis, and in particular relates to a kit for typing and detecting varicella-zoster virus and its production method and application. Background technique [0002] Varicella-zostervirus (Varicella-zostervirus, VZV) is a virus belonging to the genus Varicellavirus in the family Herpesviridae, and is the common pathogen of clinical varicella and herpes zoster. When a patient is first infected with VZV, chickenpox symptoms will occur, and a lifelong latent infection will occur. After several years, when the body's immunity declines, the latent virus may reactivate to cause herpes zoster. If chickenpox is not diagnosed and treated in time, it will cause secondary varicella pneumonia, encephalitis, myocarditis, etc., and even cause death, while herpes zoster will bring severe pain to patients for a long time, bring a lot of inconvenience to patients' lives, and greatly increase public health ...

AI Technical Summary

Problems solved by technology

[0003] Looking back on the previous clinical diagnosis methods of VZV infection, it is found that clinicians mainly rely on typical clinical symptoms and skin lesions to diagnose VZV infection, but other viruses (such as coxsackie virus, herpes simplex virus) infection can also cause chickenpox Pseudomonas rash makes differential diagnosis difficult

However, the existing laboratory diagnostic methods include virus culture, direct fluorescent antibody analysis, PCR, and serum antibody detection, all of which require large sample size, complicated operation, slow speed, incapable of batch detection, multiple detection, low sensitivity, etc. Disadvantages, these are plagued by clinical diagnosis and treatment, prevention

Among them, virus culture is the gold standard for the diagnosis of VZV infection, but it requires special laboratory conditions, complex operation, takes several days, and the positive rate is low; direct fluorescent antibody analysis is cumbersome, requires professional and experienced technicians, and only one time One specimen can be detected, but high-throughput detection is not possible; conventional PCR detection cannot be used for batch and multiple detection, and multi-tube detection is required for typing, which is cumbersome and time-consuming; serum antibody detection is a commonly used clinical method for virus diagnosis, but VZV antibody cannot be used as It is a diagnostic indicator of infection, because it has many influencing factors and is related to vaccination status

In addition, with the frequent movement of people, new outbreaks of wild strains of VZV virus in various places and changes in epidemiological characteristics such as gene mutations have also put forward new challenges for VZV prevention, requiring laboratories to quickly detect the prevalence of VZV viruses in the population. At the same time, accurate typing can be carried out to grasp the popular trend of various types of VZV, but none of the existing laboratory testing methods can carry out typing detection

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