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Application of Aphanamixoid A to lung cancer treatment medicine

A drug and lung cancer technology, applied in the field of anti-tumor drugs, can solve the problem of damage to normal tissues and cells, and achieve the effect of strong cytotoxicity and prominent substantive characteristics

Inactive Publication Date: 2013-06-05
吴俊华
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Malignant tumors are diseases that seriously threaten human life and health. Chemotherapy drugs commonly used in clinical practice can greatly damage normal tissues and cells while killing tumor cells, which limits its application.

Method used

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  • Application of Aphanamixoid A to lung cancer treatment medicine
  • Application of Aphanamixoid A to lung cancer treatment medicine
  • Application of Aphanamixoid A to lung cancer treatment medicine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] Embodiment 1: the preparation of compound Aphanamixoid A tablet involved in the present invention:

[0014] Take 20 grams of compound Aphanamixoid A, add 180 grams of conventional excipients for tablet preparation, mix well, and make 1000 tablets with a conventional tablet press.

Embodiment 2

[0015] Embodiment 2: the preparation of compound Aphanamixoid A capsules involved in the present invention:

[0016] Get 20 grams of compound Aphanamixoid A, add conventional auxiliary materials for preparing capsules such as 180 grams of starch, mix well, and pack into capsules to make 1000 tablets.

[0017] The following pharmacodynamic experiments will further illustrate its drug activity.

experiment example

[0018] Experimental example: Inhibitory effect of Aphanamixoid A on the proliferation of lung cancer cells cultured in vitro and its effect on normal human cells

[0019] Lung cancer cells in the logarithmic growth phase (non-small cell lung cancer cells A 549 , large cell lung cancer cells NCI-H460, lung squamous cell carcinoma cells HTB-8 and human lung squamous cell carcinoma cells QG-5) and human normal liver cells L02 were inoculated on 96-well culture plates, and the seeding volume per well was 3000-4000 Cells / 100uL and 6000 cells / 100uL; aseptically collect 15 mL of venous blood from healthy blood donors, anticoagulate with heparin, separate mononuclear cells by Ficoll density gradient centrifugation, prepare single cell suspension with RPMI1640 containing 10% fetal bovine serum, Adjust the cell concentration to 2×10 5 cells / mL, inoculated in 96-well culture plate, 100 uL per well. After 24 hours of cell culture on the above-mentioned culture plates, the experimental g...

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Abstract

The invention discloses application of Aphanamixoid A to lung cancer treatment medicine and belongs to the technical field of novel application of medicine. The compound has good lung cancer resistance activity and can be used for preparing the lung cancer resistance medicine. The Aphanamixoid A can significantly inhibit non-small cell lung cancer cells A 549, giant-cell lung cancer cells NCI-H460, lung squamous carcinoma cells HTB-8 and human lung pavement epithelium cancer cells QG-5, wherein the non-small cell lung cancer cells A 549, the giant-cell lung cancer cells NCI-H460, the lung squamous carcinoma cells HTB-8 and the human lung pavement epithelium cancer cells QG-5 are cultured in vitro; the inhibiting effect of the Aphanamixoid A on normal human hepatic cells LO2 and peripheral blood lymphocyte is small, and therefore obvious selectivity is achieved. The application of the Aphanamixoid A to preparation of the lung cancer treatment medicine is made public for the first time. The skeleton type is fully novel, and inhibitory activity to the lung cancer cells is surprisingly strong.

Description

technical field [0001] The invention relates to the field of antitumor drugs, in particular to the application of Aphanamixoid A in the preparation of antitumor drugs. Background technique [0002] Malignant tumors are diseases that seriously threaten human life and health. Chemotherapy drugs commonly used in clinical practice can greatly damage normal tissues and cells while killing tumor cells, which limits its application. It has become the direction and urgent task of current anticancer drug research to find highly efficient and low toxic drugs for treating tumors. Finding high-efficiency, low-toxic anticancer drugs has been considered an effective way. [0003] The present invention has confirmed by experiments that Aphanamixoid A has an effect on non-small cell lung cancer cell A cultured in vitro. 549 , large cell lung cancer cell line NCI-H460, lung squamous cell carcinoma cell line HTB-8 and human lung squamous epithelial cell line QG-5 have significant inhibitory...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/365A61P35/00
Inventor 周未末冯怡龚霞吴俊华
Owner 吴俊华
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