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3D printing bio-ink capable of constructing multi-level bionic pore structure, preparation method of 3D printing bio-ink and printing method

A 3D printing and bio-ink technology, applied in tissue regeneration, prosthesis, additive processing, etc., can solve the problems of limiting cell proliferation and stretching, achieve good bone repair effect, promote bone repair and reconstruction, and promote bone repair and reconstruction effects Effect

Pending Publication Date: 2021-09-17
SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to provide a 3D printing bio-ink that can construct a multi-level bionic pore structure for the problem of limiting cell proliferation and stretching in the scaffolds printed with existing hydrogel materials

Method used

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  • 3D printing bio-ink capable of constructing multi-level bionic pore structure, preparation method of 3D printing bio-ink and printing method
  • 3D printing bio-ink capable of constructing multi-level bionic pore structure, preparation method of 3D printing bio-ink and printing method
  • 3D printing bio-ink capable of constructing multi-level bionic pore structure, preparation method of 3D printing bio-ink and printing method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] (1) Bone defect modeling

[0044] Taking the 1cm-long segmental defect of the right femur in rabbits as an example, CT scans were performed on the full length of both femurs, and the obtained data were imported into Mimics software, a new Mask was created, and the threshold range was set to 150-1000HU to obtain bone models on both sides Data; subtract the bone defect site from the contralateral healthy bone corresponding to the bone defect site by Boolean operations to obtain the data of the bone defect site, and calculate the porosity and nutrients in the compact bone area, cancellous bone area, and cancellous bone Pore ​​area distribution; model based on the above information to obtain the model data of the target bone defect, add support structure data and adjust and determine the amount of each component in the 3D printing ink based on the data; finally export the required target bone defect in an STL format file 3D digital model of the bracket.

[0045] (2) 3D pri...

Embodiment 2

[0055] Bone defect modeling, preparation of methacrylic anhydride gelatin solid, and post-printing treatment are the same as in Example 1. The difference is that different bio-ink configuration methods and printing parameters are used.

[0056] The bio-ink configuration is specifically to digest the bone marrow mesenchymal stem cells into a suspension and count them, take 1 million bone marrow mesenchymal stem cells and centrifuge them, blow and beat the centrifuged cells with 0.5ml of sterilized hydrogel solution, and resuspend them It is a cell suspension to obtain a cell-loaded hydrogel solution; then take 0.015g tricalcium phosphate and add it to the cell-loaded hydrogel solution and mix evenly to obtain a cell-loaded and 1.5wt% inorganic osteogenic active ingredient (inorganic growth factor) 0.5ml sterilized polyethylene oxide solution was added to the hydrogel solution containing cells and inorganic growth factors and mixed evenly to obtain a 3D printing bioink with a vo...

Embodiment 3

[0060] Bone defect modeling, preparation of methacrylic anhydride gelatin solid, and post-printing treatment are the same as in Example 1. The difference is that different bio-ink configuration methods and printing parameters are used.

[0061] The bio-ink configuration is specifically to digest the bone marrow mesenchymal stem cells into a suspension and count them, take 10 million bone marrow mesenchymal stem cells and centrifuge them, blow and beat the centrifuged cells with 0.75ml of sterilized hydrogel solution, and resuspend them It is a cell suspension to obtain a cell-loaded hydrogel solution; then take 0.005g tetracalcium phosphate and add the cell-loaded hydrogel solution and mix evenly to obtain a cell-loaded and 0.5wt% inorganic growth factor hydrogel solution; Add 0.25ml of sterilized polyethylene oxide solution to the hydrogel solution loaded with cells and inorganic growth factors and mix evenly to obtain a 3D printed bio-ink with a volume ratio of 3:1; immediat...

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Abstract

The invention discloses 3D printing bio-ink capable of constructing a multi-level bionic pore structure, a preparation method of the 3D printing bio-ink and a printing method. The prepared bio-ink comprises photo-curable hydrogel, a polyethylene oxide solution, an initiator, an inorganic osteogenic active component, an organic osteogenic active component and mesenchymal stem cells, and regarding a stent printed by a 3D printing technology, the porosityis 46-70%, the macropore size is 300-1000 micrometers, the microscopic size is 10-100 micrometers, and 62-90% of growth factors and cells retained in the stent can be uniformly distributed and can be proliferated and migrated through mutually communicated pores, such that the requirements of the cells in the stent for nutrition and metabolite exchange are met, and bone repair and reconstruction of the implant stent are promoted. The 3D printing bio-ink provided by the invention has good biocompatibility and good dispersibility, and can be completely degraded, such that the printed stent not only has a good bone repair effect, but also can be automatically absorbed and discharged by a human body, which does not need to be taken out by a secondary operation, and has a relatively high clinical application value.

Description

technical field [0001] The invention belongs to the technical field of biomaterials and their preparation, in particular to the field of 3D bioprinting materials and their preparation technologies, and in particular to a 3D printing bio-ink suitable for bone regeneration and repair, containing living cells and growth factors and capable of constructing a multi-level bionic pore structure Its preparation method and printing method. Background technique [0002] Bone defects caused by car accidents, trauma, malignant tumors and other factors are common and difficult to solve in clinical practice. For this, the commonly used clinical treatment method is bone grafting. At present, the sources of commonly used grafts in bone grafting include autologous bone, allogeneic bone, xenograft bone, and various inactive artificial bones, etc., but these bone graft materials not only have limited sources, but also cause complications at the bone harvesting site Due to the high mechanical ...

Claims

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Application Information

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IPC IPC(8): A61L27/22A61L27/44A61L27/46A61L27/52A61L27/54A61L27/56A61L27/58B33Y30/00B33Y40/10B33Y50/00B33Y70/10B33Y80/00A61L27/38
CPCA61L27/58A61L27/56A61L27/52A61L27/54A61L27/227A61L27/46A61L27/446B33Y50/00B33Y70/10B33Y80/00B33Y30/00B33Y40/10A61L27/3834A61L2430/02A61L2300/414A61L2300/30C08L71/02
Inventor 周长春刘雷李明欣王文朝朱纬韬宋平李军樊渝江王科峰张兴栋
Owner SICHUAN UNIV
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