Chitin fiber reinforced collagen base bone tissue engineering scaffold with compounded human mesenchymal stem cells and preparation method

Abstract

The invention discloses a bone tissue engineering scaffold which compounds bone-like tissue engineering materials with human mesenchymal stem cells and a preparation method of the bone tissue engineering scaffold, and belongs to the technical field of tissue engineering. The bone tissue engineering material is prepared by compounding mineralization collagen (MC), polylactic acid (PLA) and natural chitin woven high-strength fiber (CHF); because of the adding of the MC, the material is good in mechanics integration, and the components of the material are close to natural bones; due to the adding of the PLA, the material is good in molding property; by adopting the CHF, the material is enhanced, and the acid degradation product of the PLA is neutralized by the degradation product; and the bone tissue engineering material can be degraded and has a three-dimensional porous structure. By adopting the scaffold formed by compounding the bone tissue engineering material with the human mesenchymal stem cells, people go through goat tibia bone defect with the bone spaces of 25mm after eight weeks, the bone defect is completely recovered after the implantation of the scaffold, and the recovery effect and the self-bone have no obvious difference; and therefore, the bone tissue engineering scaffold is hopefully applied to clinic.

Description

technical field [0001] The invention belongs to the technical field of tissue engineering. More precisely, the invention relates to a chitin fiber-reinforced collagen-based bone tissue engineering scaffold for bone marrow mesenchymal stem cells and a preparation method for repairing bone defects. Background technique [0002] Bone tissue is the largest organ in the human body and is also the most prone to defects. Every year, millions of patients with bone tissue damage need surgical treatment. Bone grafting is the traditional method for repairing bone defects. Autologous bone grafting is currently the most commonly used therapy, but the limited availability of donors limits its application and increases the suffering of patients. Allograft bone has antigenicity, especially when implanted in a large amount, it will often cause severe immune rejection. Bone tissue engineering can achieve bone regeneration and provide new methods and approaches for the repair of clinical bo...

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Problems solved by technology

However, as a material for bone tissue engineering, PLA has its own insurmountable shortcomings: 1. After being made into a three-dimensional porous material, its strength is not enough; and as the degradation progresses, the strength decreases too quickly

However, because bone tissue engineering usually requires a large amount of implanted material, the local pH value of the local tissue will drop with the local accumulation of a large amount of PLA degradation product lactic acid, which will stimulate the local tissue and cause aseptic inflammation [Daniels A, Heller A, Heller J. Toxicity of absorbable polymers proposed for fracture fixation devices. Mar. Biol. 1996;125:525-529]

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