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Composite hydrogel for promoting bone repair and preparation method and application of composite hydrogel

A composite hydrogel and bone repair technology, applied in medical science, prosthesis, tissue regeneration, etc., can solve the problems of lack of elasticity and viscosity, hard hydrogel, poor colonization effect, etc., and achieve excellent adhesion performance, Promotes healing and is safe to use

Pending Publication Date: 2020-08-25
ZHEJIANG MEDICAL COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the hydrogel formed by GelMA is hard, lacks elasticity and viscosity, and has poor colonization effect, which limits its application.

Method used

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  • Composite hydrogel for promoting bone repair and preparation method and application of composite hydrogel
  • Composite hydrogel for promoting bone repair and preparation method and application of composite hydrogel
  • Composite hydrogel for promoting bone repair and preparation method and application of composite hydrogel

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Preparation of methacrylic acid-modified gelatin (GelMA): Weigh 5 g of solid gelatin into 100 mL of pure water, stir and dissolve in a 60° C. water bath, and prepare a 5% w / v gelatin solution. Measure 3mL of methacrylic anhydride and dissolve it in 15mL of pure water to form a methacrylic anhydride solution. The methacrylic anhydride solution was slowly added dropwise to the gelatin solution, and stirred in a water bath at 60°C for 4 hours. After the reaction, the mixed solution was diluted to a solution containing 2.5% w / v gelatin, then dialyzed in pure water with a 8000-12000Da dialysis bag, and finally freeze-dried at -50°C to obtain GelMA.

Embodiment 2

[0038] Preparation of dopamine-modified sodium alginate (SA-DA): 1 g of sodium alginate was weighed and dissolved in 100 mL of deionized water to prepare a 1% w / v sodium alginate solution. Weigh 1.0g of EDC and add it to the sodium alginate solution, adjust the pH to 5.5, activate for 15min, then add 1.5g of NHS, stir for 15min, quickly add 0.5g of dopamine hydrochloride, and then use 0.1mol / L of sodium hydroxide or Adjust the pH of the reaction system with hydrochloric acid solution and control the pH value between 5 and 6, react at room temperature under nitrogen protection for 12 hours, dialyze with hydrochloric acid solution with pH=5.5 for 1 day, and then dialyze with distilled water for 2 days (molecular weight cut-off: 8000~15000Da ), and finally freeze-dried at -50°C to obtain SA-DA.

Embodiment 3

[0040] Preparation of GelMA hydrogel: the GelMA prepared in Example 1 was dissolved in PBS solution to prepare a GelMA solution with a concentration of 10% w / v. Put 0.2 mL of the above GelMA solution in a 48-well cell plate, add 0.1% w / v photoinitiator LAP, stir well, and irradiate with ultraviolet light for 1 min to obtain GelMA hydrogel.

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Abstract

The invention discloses a composite hydrogel for promoting bone repair. The composite hydrogel is prepared from the following components; methacrylic acid modified gelatin and dopamine modified sodiumalginate. In the composite hydrogel, the content of the methacrylic acid modified gelatin is 8-12% w / v, and the content of the dopamine modified sodium alginate is 0.5% w / v-2% w / v. A molecular structure of the dopamine modified sodium alginate contains a catechol structure, and the adhesion property is excellent. The composite hydrogel uses the methacrylic acid modified gelatin as a substrate ofthe hydrogel, and the mechanical properties and adhesion properties of the hydrogel are improved by incorporating the dopamine modified sodium alginate, so that the viscosity is excellent, the softness degree and elasticity are suitable, and the composite hydrogel is suitable for bone repair treatment. The results of in vitro experiments show that the composite hydrogel can load and slowly releasebiologically active factors and / or drugs that promote bone repair, good repair effect can be presented in bone defect models, and the healing of bone defect wounds can be significantly promoted.

Description

technical field [0001] The invention relates to the technical field of medical biomaterials, in particular to a composite hydrogel for promoting bone repair and its preparation method and application. Background technique [0002] Bone defect is a common clinical disease, which often occurs when the fracture fragment passes through the limb during trauma or when the inactive bone is removed during debridement of an open fracture. The need for bone repair is ubiquitous in orthopedic and neurosurgery as well as oral and maxillofacial surgery. [0003] At present, the methods for treating bone defects include autologous bone and allogeneic bone transplantation, tissue engineering technology, gene therapy rejection method, growth factor, adjuvant therapy of physical therapy and so on. As an important part of modern medicine, biomedical implants are used to replace the missing structure or function of the human body, and have a variety of clinical applications in tissue repair a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/22A61L27/20A61L27/52A61L27/58A61L27/54C08J3/075C08L89/00C08L5/04
CPCA61L27/20A61L27/222A61L27/52A61L27/54A61L27/58A61L2300/414A61L2430/02C08J3/075C08J2389/00C08J2405/04C08L89/00C08L5/04
Inventor 林海燕周艳王维倩涂业颖
Owner ZHEJIANG MEDICAL COLLEGE
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