The invention discloses a preparation method of aloe/sodium alginate double-layer hydrogel dressing. The method includes: adding plasticizer glycerol into sodium alginate aqueous solution, stirring, performing ultrasonic treatment, standing at room temperature to obtain sodium alginate gel, dripping the sodium alginate gel into a culture dish, and drying to obtain a sodium alginate substrate layer; adding aloe aqueous solution into the sodium alginate aqueous solution, stirring, performing ultrasonic treatment, and standing at room temperature to obtain aloe/sodium alginate mixed gel; dripping the aloe/sodium alginate mixed gel on the surface of the sodium alginate substrate layer, and drying; soaking the double-layer hydrogel film into CaCl2 solution for 5-20 minutes, then washing with distilled water, and drying to obtain the aloe/sodium alginate double-layer hydrogel dressing. The method has the advantages that the hydrogel dressing with high transparency and swelling rate is prepared through the simple method, and the prepared dressing has excellent antibacterial performance, can promote fibroblast proliferation ability, and has weak sensitization and no irritation to the skin.