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Method for preparing extract of adult bombay canary

A technique for the extract of Periplaneta americana, which is applied in the field of preparation of the adult extract of Periplaneta americana, can solve the problems that amino acids cannot be effectively extracted, and the total recovery rate is less than 10%, achieving no toxic side effects and good extraction effect , extract the full effect

Inactive Publication Date: 2007-05-16
肖小芹 +1
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AI Technical Summary

Problems solved by technology

[0005] Existing methods for the preparation of Periplaneta americana extract mostly use the traditional ethanol extraction method or simple water extraction method, resulting in a large number of amino acids not being effectively extracted, the total recovery rate is less than 10%, and the total amino acid content of the product is only 30%

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Example 1: Preparation method of Periplaneta americana adult extract

[0017] A water extraction:

[0018] 1. Rough extraction: Select adults of Periplaneta americana within one month, transfer them to a glass tank with water supply but withdraw their food, and raise them for 5-6 days. After the adults spit out the contents, freeze them at -20°C for 30 minutes and kill them, then wash them with 75% ethanol for a short time Wash the surface of the worm body twice with distilled water, dry it in air, cut off the wings, feet, and tentacles, weigh about 10g, put it in a mortar, add 10ml of distilled water to grind for 30min, put it in a -20℃ refrigerator for 15min, and repeat this for 3 times until the worm body is fully ground into a homogenous slurry. Transfer the homogenate into a 50ml centrifuge tube, set the volume to 40ml, centrifuge at 4000rpm for 15min, separate the upper layer of oil and the lower layer of sediment, take the middle supernatant, and centrifuge at 1...

Embodiment 2

[0024] Example 2: Acute toxicity test of Periplaneta americana extract in mice

[0025] 40 Kunming mice, half male and half male, weighing 20±2 g, were randomly divided into 4 groups, 10 mice in each group. Animals were fasted overnight before administration, and the extracts of female worms and nymphs were given intragastric administration once after numbering. Each of the two test substances was divided into four dosage groups. The largest dosage group took the original solution, and the other dosage groups were set at a distance of 1:0.8. The amount of gavage was 0.25ml / 10g body weight. Observe the toxic reaction of the animal after gavage for 14 days, and obtain the median lethal dose (LD) of the test substance to mice. 50 ).

[0026] After intragastric administration, the mice were continuously observed for 14 days without death, and the mice grew well, and there were no abnormalities in appearance, behavior, mental state, diet, urine and stool, color, skin and fur, etc...

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PUM

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Abstract

The invention relates to a method for preparing America roach adult extractive, wherein it comprises that combines alcohol extraction and water extraction techniques, while the total recycle rate can reach 25.2% and the amino acid content of recycled product can reach 58.5%. The inventive method is simple with low cost and short time. The test on rat has proved that the invention has no side effect and toxic.

Description

technical field [0001] The invention specifically relates to a preparation method of an adult extract of the American cockroach (Periplaneta americana). Background technique [0002] Cockroach (Periplaneta) is an ancient insect (cockroach), which has survived on the earth for 350 million years. It can be seen that it has the best biologically active substances, special physiological mechanism, and super life information maintenance system in its body, so that it can become the oldest and most successful insect group in the world. . "Chinese Pharmacy Dictionary", "Chinese Medicine Dictionary", "Chinese Medicinal Animals", "National Chinese Herbal Medicine Compilation" and other recent and modern pharmacy monographs all record that cockroaches have "promoting blood circulation, dispelling blood stasis, dissolving accumulation, eliminating malnutrition, and detoxification". , diuretic, detumescence” and other functions, and is used to treat boils, throat moths, carbuncles, sn...

Claims

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Application Information

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IPC IPC(8): A61K35/64A61P17/02
Inventor 肖小芹汪世平罗臣周松华徐绍锐吴仕筠
Owner 肖小芹
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