Method for restoring oil polluted wet land by combination of plant and microorganism

Abstract

The invention relates to a method for restoring oil polluted wet land by combination of plant and microorganism. The method concretely includes: soil of oil polluted wet land to be restored is collected, autochthonous oil-munching bacterium screening is carried out by utilizing an inorganic salt culture medium containing the oil contamination, the culture and screening of autochthonous oil-munching bacterium are enlarged, and the autochthonous oil-munching bacterium is made into active bacterium preparation with OD600 of about 1.5 by adopting nutrition substrate with the urea concentration of 40g / L and the calcium superphosphate concentration of 15g / L; scripus triqueter seedling or scripus triqueter seed is planted in the oil polluted wetland soil, and planting density is 2500-5000 plants / m2; when seedling grows vigorously, the active bacterium preparation obtained by the step a is added into the root system soil planted with scripus triqueter seedling according to the mass ratio of about 100:1, when flowering period and mature period are finished, under the combined action of oil-munching bacteria and root system of plant, the oil polluted wet land is restored. Compared with the traditional soil management method, the method of the invention has the advantages of less investment, less work amount, low technical requirement and no secondary pollution. The restoring process not only can not destroy soil entironment but also is beneficial to improving soil degradation and productivity reduction which are caused by petroleum hydrocarbon pollution and recovering and improving biodiversity of soil.

Description

technical field [0001] The invention relates to a method for jointly repairing oil polluted wetlands by plants and microorganisms. Background technique [0002] In the process of large-scale oil extraction, smelting, transportation, use, and treatment, accidents such as pollution, omission, blowout, and oil pipeline leakage occur frequently, resulting in serious soil pollution [Document 1: Liu Guoliang, Su Youming, etc. Research on bioremediation of oil-contaminated soil New progress [J]. Chemical and biological engineering, 2008, 25 (1): 1-4]. After petroleum hydrocarbons enter the soil, they will destroy the soil structure, disperse the soil particles, and reduce the soil water permeability. The rich reactive groups can combine with inorganic nitrogen and phosphorus to limit nitrification and dephosphorylation, so that the soil can effectively nitrogen and phosphorus. content decreased. Among them, polycyclic aromatic hydrocarbons, etc., are enriched in organisms through...

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Problems solved by technology

However, when the concentration of oil entering the soil is too high, the soil pores will be blocked, which will affect the air permeability and cause adverse effects on microorganisms. Moreover, the active surface of a large amount of soil structure will be wrapped by oil, which will make microorganisms lose a good activity place. The activity is inhibited, and its degradation ability is reduced [Document 2: Zeng Xianjun, Liu Dengkui. Research progress of microbial remediation of oil-contaminated soil [J]. Hunan Agricultural Science 2006, (2): 36-38, 39]

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