Chromium resistant paenarthrobacter nicotinovorans M23A and application thereof

A petroleum hydrocarbon degrading bacteria and petroleum hydrocarbon technology, applied in the direction of bacteria, the restoration of contaminated soil, and methods based on microorganisms, can solve the problems of affecting soil permeability, reducing the value of land cultivation, and endangering plant growth, etc., to achieve rich Effects of strain resources, great research value and application prospects

Active Publication Date: 2019-08-30
THIRD INST OF OCEANOGRAPHY MINIST OF NATURAL RESOURCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to defects in technology and management, a large amount of petroleum products flow into the soil directly or indirectly, thereby polluting the soil environment.
The leaked petroleum hydrocarbons are poured into the voids of the soil, affecting the permeability of the soil, destroying the soil water phase, gas phase and solid phase structure of crude oil, affecting the growth of microorganisms in the soil, and also affecting the respiration and moisture of plant roots in the soil. Absorption of nutrients, even rotten and necrotic plant roots, seriously endangering the growth of plants and reducing the value of land cultivation in this area

Method used

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  • Chromium resistant paenarthrobacter nicotinovorans M23A and application thereof
  • Chromium resistant paenarthrobacter nicotinovorans M23A and application thereof
  • Chromium resistant paenarthrobacter nicotinovorans M23A and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Example 1: Morphological characteristics of a strain of chromium-resistant petroleum hydrocarbon degrading bacteria (Paenarthrobacter nicotinovorans) M23A

[0019] Streak inoculate a single colony into LB solid medium, place the plate upside down in a constant temperature incubator, and incubate at 28°C for 72h. Gram staining of the bacterium was positive, the biological characteristics were non-fermentative, obligate aerobic, and the bacterium was non-bacillus. The colonies are round, off-white and translucent, with smooth and moist surface, regular edges, no halo, raised center, 1-3mm in diameter.

Embodiment 2

[0020] Example 2: Screening and Identification of a Chromium-resistant Petroleum Hydrocarbon Degrading Bacteria (Paenarthrobacter nicotinovorans) M23A

[0021] (1) On-site collection of soil samples about 5cm below the surface of the petroleum hydrocarbon polluted area of ​​the Macao Power Plant (E113°55′, N22°20′), after preliminary removal of impurities such as grass roots and stones, sent to the laboratory at low temperature for further investigation Analyze and screen the sample source of the target strain.

[0022] (2) Take a 250mL Erlenmeyer flask, wash it, fill it with 90mL deionized water and add 25 glass beads with a diameter of 0.5cm, seal it, and sterilize under high pressure at 121°C for 20min. After the system cooled down to room temperature, put 10.0 g of the aforementioned soil samples into the Erlenmeyer flask in the ultra-clean workbench, re-seal it, and shake it in a constant temperature shaker at 28°C for about 30 minutes to fully release the microorganisms ...

Embodiment 3

[0025] Embodiment 3: Determination of petroleum hydrocarbon degradation ability of chromium-resistant petroleum hydrocarbon degrading bacteria (Paenarthrobacter nicotinovorans) M23A

[0026] (1) Prepare LB liquid medium, and pick a single colony of chromium-resistant petroleum hydrocarbon degrading bacteria (Paenarthrobacter nicotinovorans) M23A on the LB solid plate to inoculate in LB liquid medium, and cultivate overnight, which is the M23A seed solution.

[0027] (2) With 1% as the inoculum amount, the M23A seed solution was added to the inorganic salt liquid medium containing 10% crude oil: diesel oil = 1: 1, shaken and cultivated at 150rpm and 28°C in a shaker, and samples were taken every 3d and used The degradation rate of petroleum hydrocarbons in the culture solution was determined by gravimetric method. The experimental period was 15 days. The experimental results are shown in figure 1 .

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Abstract

The invention discloses chromium resistant paenarthrobacter nicotinovorans M23A and an application thereof, and relates to paenarthrobacter nicotinovorans. The paenarthrobacter nicotinovorans is preserved in the China Center for Type Culture Collection on 27 November 2018, the address is Wuhan University, Wuhan, China, the postcode is 430072, and the preservation number in the preservation centeris CCTCC NO:M 2018833. The invention relates to an application of the paenarthrobacter nicotinovorans for governing petroleum hydrocarbon pollution of soil. The separation and the application of the paenarthrobacter nicotinovorans (Paenarthrobacter nicotinovorans) M23A further enrich strain resources in the field, the research blank in the respect is effectively filled, a feasible scheme is provided for practice work of governing mixed petroleum hydrocarbon pollution environment, and enormous research value and application prospect are displayed.

Description

technical field [0001] The invention relates to a petroleum hydrocarbon degrading bacterium, in particular to a chromium-resistant petroleum hydrocarbon degrading bacterium (Paenarthrobacter nicotinovorans) M23A and application thereof. Background technique [0002] Petroleum pollution refers to the pollution caused by oil leakage and discharge during the exploration, exploitation, transportation, loading and unloading, processing, storage and use of petroleum hydrocarbons and their derivatives. The main types include: soil pollution, marine pollution (H.S.El - Sheshtawy, N.M. Khalil, W. Ahmed, R.I. Abdallah. Monitoring of oil pollution at Gemsa Bay and bioremediation capacity of bacterial isolates with biosurfactants and nanoparticles. Marine Pollution Bulletin 87, 191-200 (2014).). With the prosperity and development of our country's social economy, the demand for oil is increasing day by day, which promotes the rapid development of oil-related industries and causes increa...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20B09C1/10C12R1/01
CPCB09C1/10C12N1/205C12R2001/01
Inventor 王万鹏邵宗泽唐晨
Owner THIRD INST OF OCEANOGRAPHY MINIST OF NATURAL RESOURCES
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