A Chromium-resistant Petroleum Hydrocarbon Degrading Strain tph3-32 and Its Application

A petroleum hydrocarbon and chromium-resistant technology, applied to the petroleum hydrocarbon degrading strain Tph3-32 and its application field, can solve the problems of uncontrollable risk, high cost, long cycle, etc., and achieve rich strain resources, great research value and application prospect Effect

Active Publication Date: 2021-01-01
XIAMEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, for petroleum hydrocarbon pollutants and Cr 6+ The physical method, chemical method, biological treatment technology and the combined application of different technologies have been relatively common, but the petroleum hydrocarbon pollutant-Cr 6+ Research on compound pollution is rarely reported
The existing technical route has many disadvantages such as high cost, long cycle, easy to cause secondary pollution, uncontrollable risk, etc.

Method used

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  • A Chromium-resistant Petroleum Hydrocarbon Degrading Strain tph3-32 and Its Application
  • A Chromium-resistant Petroleum Hydrocarbon Degrading Strain tph3-32 and Its Application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Example 1: Morphological characteristics of petroleum hydrocarbon degradation strain (Terrabacter sp.) Tph3-32

[0018] Streak inoculate a single colony into LB solid medium, place the plate upside down in a constant temperature incubator, and incubate at 28°C for 72h. Gram staining of the bacterium was positive, the biological characteristics were non-fermentative, obligate aerobic, and the bacterium was non-bacillus. It is round, off-white and translucent, with smooth and moist surface, regular edges, no halo, raised center, 0.7-1.2mm in diameter.

Embodiment 2

[0019] Example 2: Screening and Identification of Petroleum Hydrocarbon Degrading Strain (Terrabacter sp.) Tph3-32

[0020] (1) On-site collection of soil samples about 5cm below the surface of the petroleum hydrocarbon polluted area (E113°55′, N22°20′) of the Macao Power Plant, after preliminary removal of impurities such as grass roots and stones, sent to the laboratory at low temperature as the next step One-stage screening of sample sources for functional strains.

[0021] (2) Under laboratory conditions: take a 250mL Erlenmeyer flask, wash it, fill it with 90mL deionized water and add 25 glass beads with a diameter of 0.5cm, seal it, and autoclave it at 121°C for 20min. After the system cooled down to room temperature, put 10.0 g of the aforementioned soil samples into the Erlenmeyer flask in the ultra-clean workbench, re-seal it, and shake it in a constant temperature shaker at 28°C for about 30 minutes to fully release the microorganisms in the soil pores. The suspensi...

Embodiment 3

[0023] Embodiment 3: Petroleum hydrocarbon degradation bacterial strain (Terrabacter sp.) Tph3-32 is to Cr 6+ and Determination of Diesel Degradation Rate

[0024] (1) Prepare LB liquid medium, and pick a single colony of petroleum hydrocarbon degrading strain (Terrabactersp.) Tph3-32 on the LB solid plate, inoculate it in LB liquid medium, and cultivate it overnight, which is Tph3-32 seed solution . After sterilizing 10g of crude oil and 90mL of inorganic salt liquid medium, mix them under aseptic conditions, and add potassium dichromate powder dried to constant weight to make the Cr in the system 6+ It is 150mg / L (the value is determined by the preliminary experiment). Inject the strain seed liquid at a ratio of 1%, cultivate at 28°C and 150rpm for 15 days, take samples every 3 days, and measure Cr 6+ and the content of crude oil, and calculate the degradation rate at the corresponding time point.

[0025] (2) The results showed that the petroleum hydrocarbon degrading s...

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Abstract

A chromium-resistant petroleum hydrocarbon-degrading strain Tph3‑32 and its application, involving petroleum pollution. It was deposited in the China Center for Type Culture Collection on November 12, 2018, and the deposit number of the collection center is CCTCC NO: M 2018787. A chromium-resistant petroleum hydrocarbon degrading strain (Terrabacter sp.) Tph3‑32 in the treatment of crude oil and Cr 6+ Application of compound polluted soil treatment. The isolation and application of petroleum hydrocarbon degrading bacteria (Terrabacter sp.) Tph3‑32 has further enriched the strain resources in this field, effectively filled the research gap in this field, and provided a set of feasible solutions for the practical work of controlling polluted environment , showing great research value and application prospect.

Description

technical field [0001] The invention relates to petroleum pollution, in particular to a chromium-resistant petroleum hydrocarbon degrading bacterial strain (Terrabactersp.) Tph3-32 and its application. Background technique [0002] Petroleum pollution refers to the pollution caused by leakage and discharge of petroleum hydrocarbons and their derivatives during exploration, exploitation, transportation, loading and unloading, processing, storage and use. Its main types include: soil pollution, marine pollution (H.S.El-Sheshtawy, N.M.Khalil, W.Ahmed, R.I.Abdallah. Monitoring of oil pollution at Gemsa Bay and bioremediation capacity of bacterial isolates with biosurfactants and nanoparticles. Marine Pollution Bulletin 87, 1901-200 (2 )). With the prosperity and development of our country's social economy, the demand for oil is increasing day by day, which has promoted the rapid development of oil-related industries, but it is undeniable that this is also one of the most import...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20B09C1/10A62D3/02C12R1/01A62D101/20A62D101/43
CPCA62D3/02A62D2101/20A62D2101/43B09C1/10C12N1/205C12R2001/01
Inventor 赵苒唐晨王万鹏陈小旋郭东北范春张敏李佳瑶朱靓
Owner XIAMEN UNIV
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