Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A Strain of Bacillus thuringiensis and Its Application

A technology of Bacillus aureus and Thuringia, applied in the field of microorganisms, can solve the problems of few reports, achieve the effects of improving iron nutrition, enhancing root activity, and increasing dry and wet weight

Active Publication Date: 2021-05-25
SHANDONG AGRICULTURAL UNIVERSITY
View PDF7 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] However, the inventors have found that there are fewer reports on plant growth-promoting bacteria, particularly bacillus, which have a significant growth-promoting effect on ginger

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A Strain of Bacillus thuringiensis and Its Application
  • A Strain of Bacillus thuringiensis and Its Application
  • A Strain of Bacillus thuringiensis and Its Application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] Embodiment 1: strain identification

[0051] In the present invention, a bacterial strain that can promote the growth of ginger obtained through screening is determined to be Bacillus thuringiensis through identification of 16S rDNA and physiological and biochemical indicators. like figure 1 and 2 Shown, the bacterium colony and thalline characteristics of described Bacillus thuringiensis (Bacillus thuringiensis) TA-201 are: after cultivating on LB medium for 24h, bacterium colony is round and regular, milky white, raised, opaque; thalline is long rod Shaped, spore-forming, Gram-positive. The physiological and biochemical characteristics of the strain are: positive starch hydrolysis test, positive gelatin liquefaction test, positive glucose fermentation test, negative arabinose fermentation test, positive mannitol fermentation test, and positive xylose fermentation test.

[0052] The 16S rDNA sequence of the Bacillus thuringiensis TA-201 is as follows:

[0053] GTCG...

Embodiment 2

[0054] Embodiment 2: Bacillus thuringiensis (Bacillus thuringiensis) TA-201 is to the growth-promoting test of ginger

[0055] The preparation method of the bacterial solution: transfer the bacteria preserved on the slant to a test tube containing 5mL LB liquid medium, and culture it in a constant temperature shaker for 12 hours, the temperature: 37°C, the speed: 180rpm; the activated bacterial solution Inoculate 1% of the inoculum into 50mL LB liquid medium, put it into a shaker for 24 hours, temperature: 37°C, rotation speed: 180rpm, and make a bacterial solution.

[0056] Among them, LB liquid medium: yeast extract, 0.5%, peptone, 1%, sodium chloride, 1%, distilled water, 1000mL. Sterilize at 121°C for 20 minutes, pH7.0.

[0057] The ginger seedlings were transplanted into the potting soil, and after the ginger seedlings were established, 2 mL of the bacterial solution was diluted (the dilution factor was 100 times), and then the root irrigation treatment was carried out w...

Embodiment 3

[0060] Embodiment 3: Bacterial strain TA-201 produces protease, siderophore test

[0061] Detect the ability of Bacillus thuringiensis (Bacillus thuringiensis) TA-201 to produce protease and siderophore, the results are shown in Table 2-3 and Figure 4-5 shown.

[0062] Table 2 protease production results

[0063]

[0064] Table 3 siderophore quantitative results

[0065]

[0066] In summary, the Bacillus thuringiensis screened by the present invention has functions such as producing protease and siderophore, and the strains screened by the present invention are studied on the growth-promoting effect of ginger under pot conditions. The adopted test method is closer to the actual application state. Taking ginger grown in soil as a test sample is close to the agricultural planting method of ginger, which also strongly shows that the effect of promoting the growth of ginger in production and planting is very obvious. Simultaneously, the present invention detects the pott...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a strain of Bacillus thuringiensis and its application, belonging to the technical field of microorganisms. The strain was deposited in the General Microbiology Center of the China Committee for the Collection of Microorganisms on December 19, 2019, and its biological preservation number is CGMCC No.19222. The Bacillus thuringiensis of the present invention has functions such as producing protease and siderophore, and the Bacillus thuringiensis obtained by screening in the present invention can obviously improve the iron nutritional status of the ginger growth period, enhance the root activity of the ginger, promote the growth of the ground diameter of the ginger, and improve Plant biomass increases the dry and wet weight of ginger stems and leaves, thus proving the growth-promoting effect of the bacterial strain provided by the present invention, and enriching the strain resources of plant growth-promoting bacteria, so it has good practical application value.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a strain of bacillus thuringiensis and its application. Background technique [0002] The information disclosed in the Background of the Invention is only intended to increase the understanding of the general background of the invention, and is not necessarily to be taken as an acknowledgment or any form of suggestion that the information constitutes the prior art that is already known to those skilled in the art. [0003] Microorganisms are closely related to agriculture (such as soil fertility, crop rhizosphere micro-ecological environment), and the research and application technologies of various micro-ecological preparations have been continuously reported, and plant growth-promoting bacteria (PGPR) is one of them. PGPR is a free-living soil bacterium that can promote the dissolution of minerals, which helps to enhance the resistance of host plants and the ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/23A01P21/00C12R1/07
CPCA01N63/00C12N1/20C12N1/205C12R2001/075
Inventor 丁延芹汪成墙董建海厉启梅孙永淑邴辉
Owner SHANDONG AGRICULTURAL UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com