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A protease-producing, siderophore-producing bacillus mycoides and application thereof

The technology of Bacillus mycoides and strains is applied in the field of microorganisms to achieve the effects of enhancing root vigor, improving iron nutrient status and promoting growth

Active Publication Date: 2021-04-02
SHANDONG AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are many studies on ginger antibacterial bacteria, but there are few reports on growth-promoting bacteria that promote ginger growth and improve iron nutrition.

Method used

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  • A protease-producing, siderophore-producing bacillus mycoides and application thereof
  • A protease-producing, siderophore-producing bacillus mycoides and application thereof
  • A protease-producing, siderophore-producing bacillus mycoides and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0059] Embodiment 1: strain identification

[0060] A strain that can promote the growth of ginger was screened and identified as Bacillus mycoides by 16S rDNA and physiological and biochemical indicators. Such as figure 1 and 2 Shown, the bacterium colony and thalline characteristics of described Bacillus mycoides (Bacillus mycoides) TA-203 are: after being cultivated on LB medium for 24h, bacterium colony is round and regular, milky white, raised, opaque; Rod-shaped, spore-forming, Gram-positive.

[0061] The physiological and biochemical characteristics of the strain are: positive starch hydrolysis test, positive gelatin liquefaction test, positive glucose fermentation test, negative arabinose fermentation test, positive mannitol fermentation test, and positive xylose fermentation test.

[0062] The 16S rDNA sequence of the Bacillus mycoides TA-203 is as follows:

[0063] AGGGCCTGCGGGGTGCTATACATGCAAGTCGAGCGAATGGATTAAGAGCTTGCTCTTATGAAGTTAGCGGCGGACGGGTGAGTAACACGTGGGTAACCT...

Embodiment 2

[0064] Embodiment 2: Bacillus mycoides (Bacillus mycoides) TA-203 is to the growth-promoting test of ginger

[0065] The preparation method of the bacterial solution: transfer the bacteria preserved on the slant to a test tube containing 5mL LB liquid medium, and culture it in a constant temperature shaker for 12 hours, the temperature: 37°C, the speed: 180rpm; the activated bacterial solution Inoculate 1% of the inoculum into 50mL LB liquid medium, put it into a shaker for 24 hours, temperature: 37°C, rotation speed: 180rpm, and make a bacterial solution.

[0066] Among them, LB liquid medium: yeast extract, 0.5%, peptone, 1%, sodium chloride, 1%, distilled water, 1000mL. Sterilize at 121°C for 20 minutes, pH7.0.

[0067] Transplant the ginger seedlings into the potting soil, take 2mL bacterial solution to dilute after the ginger seedlings are established (the dilution factor is 100 times), and then use 200mL diluted bacteria for each ginger in the pot for root irrigation tr...

Embodiment 3

[0070] Embodiment 3: Bacterial strain TA-203 produces protease, siderophore test

[0071] Detect the ability of Bacillus mycoides (Bacillus mycoides) TA-203 to produce protease and siderophore, the results are shown in Table 2-3 and Figure 4-5 shown.

[0072] Table 2 protease production results

[0073]

[0074] Table 3 siderophore quantitative results

[0075]

[0076] In summary, the Bacillus mycoides screened by the present invention has the effects of producing protease, siderophore, etc., and the strains screened by the present invention have the effect of promoting the growth of ginger under pot conditions, and the adopted test method is closer to the actual application state. By directly planting ginger in soil as a test sample, it is close to the agricultural planting method of ginger, which also strongly shows that the effect of promoting the growth of ginger in production and planting is very obvious. Simultaneously, the present invention detects potted gin...

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Abstract

The invention provides a protease-producing and siderophore-producing bacillus mycoides and its application, belonging to the technical field of microorganisms. The strain was deposited in the General Microbiology Center of the China Committee for the Collection of Microorganisms on December 19, 2019, and its biological deposit number is CGMCC No.19224. The Bacillus mycoides of the present invention has the functions of producing protease and siderophore, etc. The Bacillus mycoides screened and obtained by the present invention can obviously improve the iron nutritional status of the ginger growth period, enhance the root activity of the ginger, and promote the growth of the ground diameter of the ginger , increase plant biomass, increase the dry and wet weight of ginger stems and leaves, thereby proving the growth-promoting effect of the bacterial strain provided by the present invention, and effectively enriching the strain resources of plant growth-promoting bacteria.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a protease-producing and siderophore-producing Bacillus mycoides and an application thereof. Background technique [0002] The information disclosed in the Background of the Invention is only intended to increase the understanding of the general background of the invention, and is not necessarily to be taken as an acknowledgment or any form of suggestion that the information constitutes the prior art that is already known to those skilled in the art. [0003] Ginger is not only a common raw material of Chinese medicinal materials, but also an indispensable condiment in daily life. With the increasing demand for ginger, there are more and more planting areas. As of 2018, the planting area of ​​ginger in China reached 299,000 hectares 2 . With the promotion of planting, the soil-borne diseases of ginger have become more and more serious; the long-term use of ch...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/22A01P21/00A01G7/06A01G22/25A01B79/02C05F11/08C09K17/14C12R1/07C09K101/00
CPCA01B79/02A01G7/06A01N63/00A01G22/25C05F11/08C09K17/14C09K2101/00C12N1/20C12N1/205C12R2001/07
Inventor 丁延芹邴辉董建海厉启梅孙永淑王文团
Owner SHANDONG AGRICULTURAL UNIVERSITY
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