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Bacillus thuringiensis generating protease and siderophore and application of bacillus thuringiensis

A technology of Bacillus chrysogenum and Thuringia, which is applied in the field of microorganisms to achieve the effects of enhancing root activity, improving iron nutrition status, and increasing dry and wet weight

Active Publication Date: 2020-05-08
SHANDONG AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At present, there are many reports about protease-producing bacteria and siderophores produced by Bacillus, but there are few reports about Bacillus producing protease, siderophore and significant growth-promoting effect on ginger

Method used

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  • Bacillus thuringiensis generating protease and siderophore and application of bacillus thuringiensis

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Experimental program
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Effect test

Embodiment 1

[0061] Embodiment 1: strain identification

[0062] In the present invention, a bacterial strain that can promote the growth of ginger obtained through screening is determined to be Bacillus thuringiensis through identification of 16S rDNA and physiological and biochemical indicators. like figure 1 and 2 Shown, the bacterium colony and thalline characteristics of described Bacillus thuringiensis (Bacillus thuringiensis) TA-202 are: after cultivating on LB medium for 24h, bacterium colony is round and regular, milky white, convex, opaque; thalline is long rod Shaped, spore-forming, Gram-positive.

[0063] The physiological and biochemical characteristics of the strain are: positive starch hydrolysis test, positive gelatin liquefaction test, positive glucose fermentation test, negative arabinose fermentation test, positive mannitol fermentation test, and positive xylose fermentation test.

[0064] The 16S rDNA sequence of the Bacillus thuringiensis TA-202 is as follows:

[0...

Embodiment 2

[0066] Embodiment 2: Bacillus thuringiensis (Bacillus thuringiensis) TA-202 is to the growth-promoting test of ginger

[0067] The preparation method of the fermentation broth: transfer the strains preserved on the slant to a test tube containing 5mL LB liquid medium, and culture it in a constant temperature shaker for 12 hours, the temperature: 37°C, the rotation speed: 180rpm; the activated bacterial liquid Inoculate 1% of the inoculum into 50 mL of LB liquid medium, put it into a shaker for 24 hours, temperature: 37° C., rotation speed: 180 rpm, to prepare a fermentation broth.

[0068] Among them, LB liquid medium: yeast extract, 0.5%, peptone, 1%, sodium chloride, 1%, distilled water, 1000mL. Sterilize at 121°C for 20 minutes, pH7.0.

[0069] The ginger seedlings were transplanted into the potting soil, and 2 mL of bacterial solution was taken to dilute the ginger seedlings (the dilution factor was 100 times), and then 200 mL of the diluted bacterial solution was used fo...

Embodiment 3

[0072] Embodiment 3: Bacterial strain TA-202 produces protease, siderophore test

[0073] Detect the ability of Bacillus thuringiensis (Bacillus thuringiensis) TA-202 to produce protease and siderophore, the results are shown in Table 2-3 and Figure 4-5 shown.

[0074] Table 2 protease production results

[0075]

[0076] Table 3 siderophore quantitative results

[0077]

[0078]In summary, the Bacillus thuringiensis screened by the present invention has the effects of producing protease, siderophore, etc., and the strains screened by the present invention have the effect of promoting growth of ginger under pot conditions. The test method adopted is closer to the actual application state. Ginger planted directly in the soil is used as a test sample, which is close to the agricultural planting method of ginger, which also strongly shows that the effect of promoting the growth of ginger in production and planting is very obvious. Simultaneously, the present invention d...

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Abstract

The invention provides bacillus thuringiensis generating protease and siderophore and application of the bacillus thuringiensis and belongs to the technical field of microorganisms. The strain is preserved in the China General Microbiological Culture Collection Center on December 19, 2019 and has a biological preservation number of CGMCC No.19224. The bacillus thuringiensis has a function of generating protease, siderophore and the like, can be used for remarkably improving the iron nutritional status of ginger in the growth period of raw ginger, enhancing the root vitality of the raw ginger,promoting growth of raw ginger ground diameter, improving the plant biomass and increasing the dry / wet weight of stems and leaves of the raw ginger so as to prove the growth promoting effect of the strain. The bacillus thuringiensis generating protease and siderophore has an excellent practical application value.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a protease-producing and siderophore-producing bacillus thuringiensis and its application. Background technique [0002] The information disclosed in the Background of the Invention is only intended to increase the understanding of the general background of the invention, and is not necessarily to be taken as an acknowledgment or any form of suggestion that the information constitutes the prior art that is already known to those skilled in the art. [0003] Ginger is one of the main economic fruits and vegetables in my country. The planting area in China is very extensive. my country has also become the country with the largest ginger planting area and the largest production volume in the world. According to statistics, in 2018, the planting area of ​​ginger in China reached 299,000 hm 2 Among them, Shandong Province has the largest planting area in the north. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/23A01P21/00C12R1/07
CPCA01N63/00C12N1/205C12R2001/07
Inventor 汪城墙丁延芹杜秉海邴辉姚良同刘凯
Owner SHANDONG AGRICULTURAL UNIVERSITY
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