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A Lysing Pseudomonas Chlorite and Its Application

A technology of Pseudomonas and chlorous acid, applied in the field of microorganisms, can solve problems such as unreported Pseudomonas, and achieve the effects of enriching bacterial resources, promoting growth, and fast growth.

Active Publication Date: 2021-06-04
SHANDONG AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] However, although there have been many reports on growth-promoting bacteria, so far there has been no report on Pseudomonas bacteria that are resistant to salt and alkali and have multiple growth-promoting functions

Method used

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  • A Lysing Pseudomonas Chlorite and Its Application
  • A Lysing Pseudomonas Chlorite and Its Application
  • A Lysing Pseudomonas Chlorite and Its Application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] Example 1: Strain identification

[0056] The present invention identifies a strain having a hydrochloric base having a salt-resistant base obtained by a screen selected, and identified by 16SRDNA and physiological and biochemical index, the strain is determined as a pseudomonaschloritidismutans. Among them, if figure 2 As shown, the colonies and bacteria characterized by Pseudomonaschloritidismutans 6L11: After culturing 24 h in Lb medium, the bacteria is circular, surface circular projections, smooth, humid, yellowish yellow , Marginal rules; bacteria is rod, Gram-negative, no spores. The physiological and biochemical characteristics of the strain are: contact enzyme positive, VP experiment negative, D-glucose positive, L-arabic sugar, D-mannool positive, hydrolyzed amylase positive, citrate utilization positive; the mirror detection effect of this colony Figure figure 1 Indicated.

[0057] The 16S rDNA sequence of Pseudomonas chloritidismut 6L11 is as follows:

[0058] C...

Embodiment 2

[0059] Example 2: Pseudomonas CHSeudomonaas Chloritidismutans 6L11 Greenhouse Promotional Test

[0060] The matrix of wheat seedlings into sterilization (salt base soil directly taken from Wudi County, Binzhou City, the soil pH is greater than 8.6, alkaline), after the wheat seeding, 1ml fermentation solution is diluted with water to 20 ml dilution, then Treatment of the amount of the fermentation liquid after 1 ml of wheat in potted plants. The control group was diluted with water to 20 mL for water, and then the wheat was treated. The control group ck.

[0061] After culturing 20D and 40D, measurement of wheat is measured, and the biomass, root development and chlorophyll content of potted wheat strains are detected, and the biomass, root development and chlorophyll content of the root part, and the results are shown in Table 1-3 and image 3 Indicated. As can be seen from the table: 6L11 can significantly promote the growth of wheat plants, for example, 20D and 40D wheat plant r...

Embodiment 3

[0070] Example 3: Test of 3 ligandulindozymes, pyrogen phosphorus, pitas and IAA

[0071]Detection of Pseudomonas chloritidismut 6L11 protease, pyrolysis, pitrifying capacity, product IAA and strain salt, alkali, and the results are 4-9 and Figure 4-7 Indicated.

[0072] Table 4 protease detection results

[0073]

[0074] Table 5 Polyophosphorus ability test results

[0075]

[0076] Table 6 strain production IAA content

[0077]

[0078] Table 7 Salt resistance test results

[0079]

[0080] Table 8 Substance of alkali resistance

[0081]

[0082] Table 9 Salt-resistant base resistance test results

[0083]

[0084] Note: In Table 8-10, the "+++" indicates that the growth is strong; ++ "indicates that the growth is better;" + "means growth;" - "means not growing

[0085] In summary, it can be seen that under potted conditions, the fermentation of the pseudocetransfills of the present invention can be treated after the seedling root of wheat, can produce proteases, gr...

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Abstract

The invention belongs to the technical field of microbes, and in particular relates to a lysed pseudomonas chlorite and application thereof. The strain was deposited in the General Microorganism Center of China Committee for Microorganism Culture Collection (Address: No. 3, Yard No. 1, Beichen West Road, Chaoyang District, Beijing, China) on August 06, 2018, and its biological preservation number is CGMCCNo.18364. Under potted conditions, use the fermented liquid of Pseudomonas of the present invention to irrigate the roots after the wheat seedlings are settled, and protease and auxin can be produced in the soil, which can degrade insoluble phosphorus and potassium in the soil, thereby enhancing the stability of the soil in a saline-alkali environment. The root activity of wheat promotes the growth of wheat plants, plant height, aboveground biomass, root biomass, root development and chlorophyll content, can improve the growth of wheat seedlings, and has a good effect on wheat grown in soil saline environment The growth-promoting effect has strongly proved the very obvious growth-promoting effect on wheat in the production and planting of saline-alkali land.

Description

Technical field [0001] The present invention belongs to the field of microbial technology, and specifically, it is specifically involved in pseudomocellosalasum and its application thereof. Background technique [0002] The information disclosed in the art of the present invention will only be intended to increase the understanding of the overall background of the present invention, and is not necessarily considered to admit or imply that the information has become a prior art known to those of ordinary skill in the art. [0003] Saline-alkali soil is a high salt content, which is not conducive to plant growth, which is distributed around the world. With the sharp increase in population and unreasonable reclamation, the original structure of soil epidermal is destroyed, resulting in land desertification and salt. Again, these saline soils affect crop yield, which limits the sustainable development of my country's rural economic industry. [0004] As the number of population incre...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C05F11/08A01G22/20C12R1/38
CPCA01G22/20C05F11/08C12N1/20C12N1/205C12R2001/38
Inventor 丁延芹汪城墙杜秉海刘凯姚良同王文团
Owner SHANDONG AGRICULTURAL UNIVERSITY
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