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Stenotrophomonas maltophilia, microbial inoculum containing stenotrophomonas maltophilia and application and diesel-degrading method thereof

A technique for oligotrophomonas, maltophilia, applied in chemical instruments and methods, microorganism-based methods, biochemical equipment and methods, etc.

Active Publication Date: 2017-03-15
粮华生物科技(北京)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The purpose of the present invention is to overcome the above-mentioned defects in the bioremediation of diesel oil pollution, to provide a Stenotrophomonas maltophilia, an inoculant containing the bacterium and its application, and a method for degrading diesel oil

Method used

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  • Stenotrophomonas maltophilia, microbial inoculum containing stenotrophomonas maltophilia and application and diesel-degrading method thereof
  • Stenotrophomonas maltophilia, microbial inoculum containing stenotrophomonas maltophilia and application and diesel-degrading method thereof

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Embodiment

[0044] The present invention will be described in detail below through examples and comparative examples, but the present invention is not limited thereby.

[0045] The experimental methods in the following examples and comparative examples are conventional methods in the art unless otherwise specified. The experimental materials used in the following examples and comparative examples were purchased from conventional biochemical reagent stores unless otherwise specified.

[0046] The formula of the basic inorganic salt liquid medium is: 1.0g / L KH 2 PO 4 ;5.0g / L K 2 HPO 4 ; 1.0g / L (NH 4 ) 2 SO 4 ;20mg / L MgSO 4 ·7H 2 O; 10mg / L CaCl 2 2H 2 O; 1mg / L FeCl 3 ;pH=7.0.

[0047] The method for determining the content of diesel oil by gas chromatography includes: the gas chromatograph model is GC-2010, purchased from SHIMADZU company, the chromatographic column is Agilent ZORBAX SB-Aq (4.6mm * 150mm * 5 μ m), and the mobile phase is 0.005mol / L H 2 SO 4 , the flow rate is 1...

Embodiment 1

[0061] This example is used to illustrate the liquid-phase diesel degradation ability of Stenotrophomonas maltophilia with the preservation number CGMCC NO: 10846 of the present invention.

[0062] Centrifuge the bacterial solution obtained in the preparation example to obtain the bacterial cells, then wash the bacterial cells with physiological saline and resuspend to obtain OD 600 For the physiological saline bacterial liquid of 1, the 1 mL of the preservation number of the present invention obtained above is inoculated into 30 mL of the basic inorganic salt containing 2.5% by weight of diesel oil with the physiological saline bacterial liquid of Stenotrophomonas maltophilia whose preservation number is CGMCC NO: 10846. In the liquid medium and 30 mL of LB liquid medium containing 2.5% by weight of diesel oil, cultivate them in a shaker at 37° C. at 170 rpm for 12 days. Gas chromatography was used to detect the diesel content, and the removal rate of diesel was calculated. ...

Embodiment 2

[0065] This example is used to illustrate the soil phase diesel oil degradation ability of Stenotrophomonas maltophilia with the preservation number of CGMCC NO: 10846 of the present invention.

[0066] 500g of uncontaminated soil samples were processed as follows:

[0067] CK group: adding deionized water until the soil moisture content is 20% by weight;

[0068] NTC group: sterilize the soil sample at 121°C for 20min, sterilize for 3 times in total, add 1 wt% NaN to the soil after cooling 3 Bacteriostasis, then add deionized water until the soil water content is 20% by weight, and mix evenly;

[0069] Group B1: According to the method of the preparation example, the bacterial liquid of the GZ-1 strain isolated above was obtained, centrifuged to obtain the bacterial cells, and then the bacterial cells were washed with physiological saline and resuspended to obtain OD 600 For 2.5 normal saline bacterial liquid, add 50mL of the aforementioned normal saline bacterial liquid of...

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Abstract

The invention relates to the bioremediation technical field of the petroleum hydrocarbon pollution, and discloses stenotrophomonas maltophilia, microbial inoculum containing the stenotrophomonas maltophilia and an application in diesel degradation and a diesel-degrading method. The preservation number of the stenotrophomonas maltophilia is CGMCC NO:10846. The diesel-degrading method comprises the following steps: contacting the stenotrophomonas maltophilia with the preservation number CGMCC NO:10846 and / or the microbial inoculum containing the stenotrophomonas maltophilia with the preservation number CGMCC NO:10846 with a diesel pollution sample, and degrading the diesel in the diesel pollution sample. The stenotrophomonas maltophilia is capable of effectively tolerating and degrading the high-concentration diesel compositions in the liquid phase and soil phase without adding carbon resources, with lower requirements for other elements, such as nitrogen and phosphorus, and is suitable for the high-concentration diesel bioremediation process of dead soil and dystrophic industrial wastewater.

Description

technical field [0001] The invention relates to the technical field of bioremediation of petroleum hydrocarbon pollution, in particular to a stenotrophomonas maltophilia, a bacterial agent containing the bacterium and its application, and a method for degrading diesel oil. Background technique [0002] Diesel is a light petroleum hydrocarbon product with a main component of C10-C24, which is widely used as fuel for vehicles, ships, generators, etc. Its composition is complex, including alkanes, alkenes, aromatic hydrocarbons and polycyclic aromatic hydrocarbons, etc., and may contain a small amount of sulfur and nitrogen compounds. Many of these components have carcinogenic, teratogenic, and mutagenic properties. [0003] Diesel oil entering the soil will change the physical and chemical properties of the soil and destroy the soil structure; at the same time, the diesel components will damage the root system of the plant and hinder the respiration of the plant root, thereby...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20B09C1/10C02F3/34C12R1/01C02F101/32
Inventor 任立伟李晓桐林森王培红
Owner 粮华生物科技(北京)有限公司
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