Application of eugenol in food-borne pathogenic bacterium in-vitro inhibition

A technology of food-borne pathogenic bacteria and eugenol, applied in application, food preservation, food science and other directions, can solve the problems of easy spoilage, easy spoilage, loss of edible value, etc.

Inactive Publication Date: 2015-08-12
JIANGSU TIANSHENG PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Meat and meat products are the main way for humans to obtain protein, but due to their high protein and high moisture characteristics, they are easy to spoil, especially in the process of storage, they are easy to spoil and lose their edible value

Method used

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  • Application of eugenol in food-borne pathogenic bacterium in-vitro inhibition
  • Application of eugenol in food-borne pathogenic bacterium in-vitro inhibition
  • Application of eugenol in food-borne pathogenic bacterium in-vitro inhibition

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preparation example Construction

[0021] The preparation method of bacterial culture medium is as follows: the Na of 0.2mol / L 2 HPO 4 、KH 2 PO 4 The solution is prepared according to the following ratio and tested with a pH meter: pH=5.0 buffer solution preparation: 0.2mol / L of KH 2 PO 4 The solution is adjusted to pH=5.0 with 0.1mol / L NaOH solution; pH=6.0 buffer solution preparation: 0.2mol / L NaOH 2 HPO 4 :0.2mol / L of KH 2 PO 4 =12.3:87.7;

[0022] Medium preparation with pH=7.0: tryptone soy agar (TSA) medium: weigh 38.0 g of TSA into 1 L of distilled water, autoclave at 121° C. for 20 min, and set aside. Tryptone Soy Broth (TSB) Medium: Weigh 30.0g of TSB into 1L of distilled water, autoclave at 121°C for 20min, and set aside.

[0023] According to the above method, use the buffer solution of pH = 5.0, 6.0 as the solvent to prepare the medium solution, then adjust the pH value with 6mol / L HCl or 0.2mol / L NaOH, and calibrate with a pH meter to obtain pH = 5.0, 6.0, 7.0 TSA or TSB medium solution. ...

Embodiment 1

[0025] Use an inoculation loop to pick up the hyphae cultured on the slant, move it to TSB medium for culture, place it on a shaker, cultivate it at 150r, 37°C for 24h, take it out and store it in a refrigerator at 4°C.

[0026] Take an appropriate amount from the activated bacteria test tube and inoculate it into the corresponding liquid medium, put it in the incubator on a constant temperature shaker for rejuvenation, cultivate for 8-10 hours, take an appropriate amount of rejuvenated bacteria and add sterile water to prepare a bacterial suspension. Microscopic counting method, so that the bacterial suspension contains viable bacteria at 10 6 ~10 7 cfu / mL.

[0027] 2-Methoxy-4-(2-propenyl)phenol is analyzed by GC / MS as a liquid sample, and the structural formula of the components detected by the mass spectrogram is shown in figure 1 , the abscissa refers to the mass-to-nucleus ratio, and the ordinate refers to the abundance of ions. The chromatographic peaks were searched...

Embodiment 2

[0029] Under the conditions of pH=5.0, pH=6.0 and pH=7.0, the MIC of 2-methoxy-4-(2-propenyl)phenol to Escherichia coli, Staphylococcus aureus, Salmonella and Shigella was determined.

[0030] 2.1 Filter paper method

[0031] The filter paper diffusion method is to place the filter paper soaked in essential oil on the surface of the agar plate coated with the experimental bacteria, the essential oil will diffuse from the center of the paper to the edge, and after a period of incubation, the diameter of the inhibition zone is measured to evaluate Bacteriostatic effect.

[0032] Using a hole punch, the qualitative filter paper was made into a small circular disc with a diameter of 6 mm. 2-methoxy-4-(2-propenyl)phenol is a liquid drug that can be used directly. Then put the configured medicine, small round paper, large filter paper, centrifuge tube, centrifuge tube rack, pipette gun, pipette tip, small Erlenmeyer bottle, waste liquid tank, alcohol lamp, tweezers under ultraviol...

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Abstract

The invention relates to application of eugenol and in particular relates to application of eugenol in food-borne pathogenic bacterium in-vitro inhibition, culture mediums of food-borne pathogenic bacterium are TSA culture mediums or TSB culture mediums with pH values being 5.0, 6.0 and 7.0; the diameter of an inhibition zone is measured for judging antibacterial activity of eugenol; and a multiple dilution method is adopted for carrying out minimal inhibitory concentration (MIC) determination on various bacteria. The application of eugenol in the food-borne pathogenic bacterium in vitro inhibition has the advantages that escherichia coli, staphylococcus aureus, salmonella and shigella are taken as research objects, MIC of 2-methoxy-4-(2-propenyl) phenol on common pathogens in food is researched, influence of pH on antibacterial effect of 2-methoxyl-4-(2-propenyl) phenol is studied, and application value of 2-methoxy-4-(2-propenyl) phenol in food antisepsis aspect is researched; and application range of 2-methoxy-4-(2-propenyl) phenol can be beneficially expanded.

Description

technical field [0001] The invention relates to the application of eugenol, in particular to the application of eugenol in the in vitro inhibition of food-borne pathogenic bacteria. Background technique [0002] Meat and meat products are the main way for humans to obtain protein, but due to their high protein and high moisture characteristics, they are easy to spoil, especially in the process of storage, they are easy to spoil and lose their edible value. The source of its corruption is microorganisms and enzymes, especially microorganisms, which can cause meat protein and fat to decompose and become rotten and rancid. It has a great impact on human production and life, and caused huge losses. Therefore, corresponding measures should be taken to control the occurrence of these factors. [0003] The basic principle of antiseptic preservation is mainly to inhibit the growth, metabolism and enzyme activity of microorganisms in meat products under the premise of reducing the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23B4/20A23L3/349
Inventor 季浩蒋兴凤
Owner JIANGSU TIANSHENG PHARMA
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