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Process for preparing bacteriostatic peptide by discarded tobacco leaf protein

A technology of antimicrobial peptides and tobacco leaves, which is applied in protein food processing, protein food ingredients, applications, etc., can solve the problems of low yield, difficulty in adapting to large-scale industrial production, and high cost, and achieve high safety, stable biological activity, and production low cost effect

Inactive Publication Date: 2006-09-06
GUANGDONG BRANCH OF CHINA TOBACCO GENERAL +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although these methods can obtain pure tobacco leaf protein, the cost is too high, and the extraction yield per unit time is low, so it is difficult to adapt to large-scale industrial production
The research work on the development and utilization of tobacco leaf protein in my country started relatively late, and only some preliminary discussions have been made on the extraction methods of tobacco leaf protein, but the technical problems of large-scale extraction of tobacco leaf protein have yet to be resolved

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Step 1: Crush low-grade tobacco leaves into 100-mesh tobacco leaf powder, mix with water at a ratio of 1:10, grind at 40°C, adjust the pH value to 7.5 with sodium hydroxide, and filter with 200-mesh filter cloth after stirring for 1 hour , and the filtrate is an alkali-extracted tobacco leaf protein solution. The filter residue was alkali-dissolved twice by the above process, filtered to remove impurities, and combined with the alkali-extracted tobacco leaf protein solution; the pH value of the alkali-extracted tobacco leaf protein solution was adjusted to 2.5 with hydrochloric acid, and after acid precipitation at 4°C for 6 hours, centrifuge at 4800rpm at 4°C After 20 minutes, protein was precipitated to obtain tobacco leaf protein slurry, and the recovery rate reached 86.71%.

[0019] The second step: Dissolve the low-grade tobacco leaf protein slurry in water to make the protein concentration reach 4%. After mixing evenly, adjust the pH value of the solution to 6.0 w...

Embodiment 2

[0022] Step 1: Crush low-grade tobacco leaves into 80-mesh tobacco leaf powder, mix with water at a ratio of 1:12, grind at 50°C, adjust the pH value to 8 with sodium hydroxide, and filter with 200-mesh filter cloth after stirring for 1 hour , and the filtrate is an alkali-extracted tobacco leaf protein solution. The filter residue was alkali-dissolved twice using the above process, filtered to remove impurities, and combined with the alkali-extracted tobacco leaf protein solution; the pH value of the alkali-extracted tobacco leaf protein solution was adjusted to 3.0 with hydrochloric acid, and after acid precipitation at 4°C for 6 hours, centrifuge at 4800rpm at 4°C Precipitate protein for 20 minutes to obtain tobacco leaf protein slurry with a recovery rate of 84.24%.

[0023] Step 2: Dissolve the low-grade tobacco leaf protein slurry in water to make the protein concentration reach 4-8%. After mixing evenly, adjust the pH value of the solution to 7.0 with 0.1M sodium hydrox...

Embodiment 3

[0026] Step 1: Crush low-grade tobacco leaves into 60-mesh tobacco leaf powder, mix with water at a ratio of 1:15, refine at 50°C, adjust the pH value to 8.5 with sodium hydroxide, and filter with 200-mesh filter cloth after stirring for 1 hour , and the filtrate is an alkali-extracted tobacco leaf protein solution. The filter residue is alkali-dissolved once again using the above process, filtered to remove impurities, and combined with the alkali-extracted tobacco leaf protein solution; the pH value of the alkali-extracted tobacco leaf protein solution is adjusted to 3.0 with hydrochloric acid, and after acid precipitation at 4°C for 10 hours, centrifuge at 4800rpm at 4°C After 20 minutes, the protein was precipitated, and the tobacco leaf protein slurry was obtained, and the recovery rate was 83.53%.

[0027] Step 2: Dissolve the low-grade tobacco leaf protein slurry in water to make the protein concentration reach 8%. After mixing evenly, adjust the pH value of the solutio...

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Abstract

The method comprises the following steps: using base solubilization and acid precipitaton method to extract protein from tobacco leaf to prepare tobacco leaf protein plasm; using composite proteinase to decompose the tobacco leaf protein plasm to prepare tobacco leaf protein enzymolysis liquid; using ultrafilter membrane to fractional separate the tobacco leaf protein enzymolysis liquid to get tobacco leaf protein bacteriostatic peptide mixed liquid; cooling drying or spray drying; getting the tobacco leaf protein bacteriostatic peptide compound which can suppress the growth of Gram-negative bacterium. The lowest bacteriostatic concentration is 0.05%. The invention uses the base solubilization and acid precipitaton method to improve the extraction rate of tobacco leaf protein, uses the biology enzymolysis to control the degradation speed of protein, and uses the ultrafilter membrane to separate enzymolysis liquid to get biology active peptide to simplify the operation technology and improve the effective concentration of biology active peptide.

Description

technical field [0001] The invention relates to a method for preparing bacteriostatic peptides from low-grade tobacco leaf protein. Background technique [0002] China is a big country in tobacco planting and production, and its planting area and output both rank first in the world. In the process of tobacco planting and processing, a large amount of tobacco waste such as low-grade tobacco leaves, tobacco stems, tobacco dust and tobacco seeds is produced, accounting for about 25% of the total tobacco leaf output. The comprehensive utilization of low-grade tobacco leaf resources has always been valued by researchers at home and abroad. There are many research reports: extracting tobacco leaf protein similar to soybean protein from low-grade tobacco leaves, as food protein source and high-quality feed additive. From the point of view of the extraction method of tobacco leaf protein, the tobacco leaf protein extracted by early research workers often has color and taste, and t...

Claims

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Application Information

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IPC IPC(8): C12P21/00A23J3/00
Inventor 赵谋明崔春饶国华
Owner GUANGDONG BRANCH OF CHINA TOBACCO GENERAL
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