Method for quickly detecting drug resistance of bacteria

Abstract

The invention relates to a method for quickly detecting drug resistance of bacteria. The method comprises the following steps: experimental groups of antibiotics with different concentrations, a positive control group and a negative control group are set, wherein the experimental groups of the antibiotics with the different concentrations refer to experimental groups of adding the antibiotics withthe different concentrations into culture mediums and adding heavy water in subsequent steps, the positive control group refers to the experimental group of adding antibiotics with a concentration of0 into a culture medium and adding heavy water in subsequent steps, and the negative control group refers to the experimental group of adding antibiotics with a concentration of 0 intp a culture medium without adding heavy water in subsequent steps, after incubation is performed, centrifugal washing is performed, samples are subjected to Raman detection, values of C-D / (C-D + C-H) are calculated respectively according to the obtained Raman spectra, a turning point of value decrease of C-D / (C-D + C-H) of the experimental groups of the antibiotics with different concentrations relative to the control groups is taken as a minimum inhibitory concentration of the antibiotics on the bacteria, and the minimum inhibitory concentration is compared with a breakpoint given in a CLSI susceptibility test standard to determine the bacterial sensitivity, intermediation or drug resistance. The method introduces a breakpoint comparison to more accurately give the sensitivity of the bacteria to the antibiotics.

Description

technical field [0001] The invention relates to a method for detecting bacteria, in particular to a method for rapidly detecting drug resistance of bacteria. Background technique [0002] The traditional methods for bacterial resistance detection mainly include disk diffusion method, broth dilution method and agar dilution method (Melvin P, Performance Standards for Antimicrobial Susceptibility Testing. CLSIM100.28, 30 (2018)). Bacterial culture and purification steps are required before the sensitivity test, and the culture takes 24 to 48 hours. The subsequent drug sensitivity test also judges the sensitivity of the bacteria to the drug by observing whether the bacteria grow and proliferate, such as the bacterial turbidity or the size of the antibacterial plaque. It takes 16-20 hours. It takes 3 to 5 days to obtain a drug susceptibility report through traditional drug susceptibility testing methods, which cannot meet the needs of acute infection doctors and patients for ra...

AI Technical Summary

Problems solved by technology

[0005] However, this method has disadvantages: after antibiotics are added to the cell culture medium, it takes a certain amount of time for the antibacterial or bactericidal effect on bacteria to take effect

Therefore, even if the bacteria are sensitive to the applied drug, the presence of the C-D peak will be detected during Raman detection, resulting in inaccurate judgment of drug sensitivity results

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