Method for treatment of fish culture sewage by microbial flocculation

A microbial flocculation and sewage technology, which is applied in biological water/sewage treatment, chemical instruments and methods, animal husbandry wastewater treatment, etc., can solve the problems of high treatment cost, achieve simplified water treatment process, simple nutritional requirements, and reduce The effect of processing costs

Inactive Publication Date: 2013-04-24
FISHERY MACHINERY & INSTR RES INST CHINESE ACADEMY OF FISHERY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Traditional fish farming wastewater purification technologies remove a large amount of nitrogen and phosphorus in this eutrophic water body as pollutants, resulting in high treatment costs

Method used

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  • Method for treatment of fish culture sewage by microbial flocculation
  • Method for treatment of fish culture sewage by microbial flocculation
  • Method for treatment of fish culture sewage by microbial flocculation

Examples

Experimental program
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Effect test

Embodiment 1

[0032] A method for treating fish farming sewage by microbial flocculation, comprising the steps of:

[0033] 1) Bacteria collection and selection: Bacillus subtilis subsp.subtilis (C GM C C 1.108) was selected as the flocculation production bacteria. The bacteria can be purchased from the China Microbial Strain Resource Bank.

[0034] 2) Activation and propagation of bacterial strains: medium formula, containing the following components by weight in 1000 parts by weight of distilled water: peptone 10.0, beef extract powder 3.0, NaCl 5.0, agar powder 14.0; the pH value of the medium is 7.5; at 121°C Sterilize for 15 minutes. Take 0.3 ml of liquid culture medium and drop it into the ampoule tube of the preserved freeze-dried bacteria (Bacillus subtilis), so that the frozen bacteria are dissolved and suspended; Cultivate at high temperature for 48 hours; use sterilized plate culture medium and the same culture conditions to complete the multiplication of the strain; convention...

Embodiment 2

[0039] A method for treating fish farming sewage by microbial flocculation, comprising the steps of:

[0040] 1) Bacteria collection and selection: Bacillus subtilis subsp.subtilis (C GM C C 1.108) was selected as the flocculation production bacteria. The bacteria can be purchased from the China Microbial Strain Resource Bank.

[0041] 2) Activation and propagation of bacterial strains: medium formula, containing the following components by weight in 1000 parts by weight of distilled water: peptone 10.0, beef extract powder 3.0, NaCl 5.0, agar powder 14.0; the pH value of the medium is 7.5; at 121°C Sterilize for 15 minutes. Take 0.5 ml of liquid culture medium and drop it into the ampoule tube of the preserved freeze-dried bacteria (Bacillus subtilis), so that the frozen bacteria are dissolved and suspended; Incubate at temperature for 48 hours. The sterilized plate culture medium and the same culture conditions are used to complete the multiplication of the strain; the mu...

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Abstract

The invention relates to a method for treatment of fish culture sewage by microbial flocculation. The method comprises the steps of: 1) strain selection: selecting Bacillus subtilis subsp. subtilis as a flocculate producing strain; 2) strain activation and propagation: sucking 0.3-0.5ml of a sterilized liquid medium, dropping it into an ampoule tube where frozen dry Bacillus subtilis subsp. subtilis is preserved so as to make the frozen strain dissolved into a suspension state; transferring a strain suspension liquid to a sterilized panel medium, conducting culturing at 30-32DEG C for 36-48h; employing a sterilized panel medium to complete strain propagation under the same culture conditions; and preparing the propagated strain into a bacterial suspension liquid by a conventional method; 3) optimization of flocculation strain culture conditions: inoculating the bacterial suspension liquid into a sterilized fish culture sewage medium according to a volume ratio, and carrying out fermentation culture on a shaking table at a constant temperature of 30DEG C and at a speed of 160rpm/min, thus obtaining a fermentation culture solution; and 4) acquiring of purified fish culture sewage: carrying out centrifugal fermentation on the culture solution to obtain a supernatant, i.e. the purified solution. The method provided in the invention adopts a microbial flocculation technique to treat fish culture sewage, integrates a plurality of effects, simplifies the water treatment process, and reduces the treatment cost.

Description

technical field [0001] The invention belongs to the technical field of sewage treatment, and in particular relates to a method for treating fish farming sewage by adopting a microbial flocculation method. Background technique [0002] Fish farming sewage contains about 20-35% of the feeding amount, 10-40% of metabolites and residual bait. The traditional fish farming sewage purification technology removes a large amount of nitrogen and phosphorus substances in this eutrophic water body as pollutants, so that the treatment cost remains high. Contents of the invention [0003] The invention aims to break through the technical bottleneck and provide a simple and high-efficiency method for treating fish farming sewage by adopting a microbial flocculation method. While fish farming sewage is used as a flocculation culture medium, organic matter can be degraded. Comprehensive utilization of sewage resources to treat waste with waste. [0004] The technical scheme of the prese...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C02F3/34C02F103/20
Inventor 杨菁管崇武陈军
Owner FISHERY MACHINERY & INSTR RES INST CHINESE ACADEMY OF FISHERY SCI
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