Agar degrading enzyme generation Bacillus sp.W2017 and application thereof

A bacillus and enzyme-degrading technology, applied in the field of microorganisms, can solve problems such as inhomogeneous products, low oligosaccharide production, and difficult separation, and achieve the effects of simple nutritional requirements, short fermentation time, and simple preparation

Inactive Publication Date: 2018-02-27
YANTAI INST OF COASTAL ZONE RES CHINESE ACAD OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] At present, the main methods of obtaining agar oligosaccharides are acid hydrolysis and enzymatic hydrolysis. The acid hydrolysis has the disadvantages of product inhomogeneity, poor repeatability, and large degree of environmental pollution, while the enzymatic hydrolysis has high efficiency, strong specif

Method used

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  • Agar degrading enzyme generation Bacillus sp.W2017 and application thereof
  • Agar degrading enzyme generation Bacillus sp.W2017 and application thereof
  • Agar degrading enzyme generation Bacillus sp.W2017 and application thereof

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preparation example Construction

[0039] One embodiment of the preparation method of easy-soluble agar, the above-mentioned agar degrading enzyme is added to the agar substrate containing 0.2-1.0% mass volume ratio, at a temperature of 30-45 ° C, 150-200r / min The soluble agar can be prepared by enzymatic hydrolysis on a shaking table for 12-24 hours.

[0040] One embodiment is used to screen the above-mentioned Bacillus sp.W2017 fermentation to produce agar-degrading enzyme medium, wherein the carbon source in the medium contains agar.

[0041] In one embodiment, the agar is extracted from red algae. Specifically, in one embodiment, the red algae may be Asparagus or Geliflower.

[0042]In one embodiment, metal ions are added to the medium, and the weight and volume percentage of metal ions added is 0.2%-1%. Specifically, in one embodiment, the culture medium is added with Fe 2+ Ions are preferably added at a concentration of 0.2% (w / v).

[0043] In one embodiment, the above-mentioned Bacillus sp.W2017 can ...

Embodiment 1

[0046] 1. Screening of Bacillus sp.W2017:

[0047] (1) Enrichment culture: pick asparagus and rot in water for 14 days.

[0048] (2) Separation and purification: spread the cultivated bacterial liquid on solid medium according to the dilution coating plate method, and select single colonies that form obvious dissolution circles on the agar plate after culturing at 37°C for 48 hours, and use conventional microbial isolation Methods The isolation and purification were repeated until the pure bacteria, namely Bacillus sp.W2017, were obtained.

[0049] The components and proportions of the solid medium used in the above enrichment and separation process are as follows:

[0050] Solid medium: tryptone 5.0g, yeast extract powder 1.0g, ferric citrate 0.02g, agar 20.0g, dilute to 1000mL with seawater.

[0051] The colony of Bacillus sp.W2017 on the solid medium is moist and smooth, with neat edges and round shape. After 48 hours of culture, a clear dissolution circle can be formed a...

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Abstract

The invention discloses Bacillus sp.W2017 which is preserved in the China Center for Type Culture Collection in the Wuhan University on the Bayi Road, Hongshan District, Wuhan, Hubei, China on May 2,2017, the zip code is 430072, and the preservation number is CCTCC No: M 2017226. The invention further relates to application of the agar degrading enzyme generation Bacillus sp.W2017. The agar degrading enzyme generation Bacillus sp.W2017 is a Gram-negative bacterium which is high in enzyme activity, stable in enzyme activity and high in enzyme yield.

Description

technical field [0001] The invention relates to the field of microorganisms, in particular to Bacillus sp.W2017 producing agar degrading enzyme and application thereof. Background technique [0002] As one of the world's three major industrial seaweed gums, agar is a kind of polymer polysaccharide with galactose as the main component. It is a polysaccharide mixture composed of neutral agarose and acidic agar ester. Agar oligosaccharides refer to oligosaccharides with a small degree of polymerization and good water solubility obtained by hydrolysis of agar, including new agar oligosaccharides with galactose as the reducing end and 3,6-endether galactose as the reducing end. end of the agar oligosaccharide. Studies have shown that agar oligosaccharides have a variety of physiological activities, such as anti-oxidation, lowering blood lipids, immune regulation, anti-allergic and inhibiting glycosidase, etc. At the same time, it has the effect of moisturizing the skin, and can ...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N9/38C12N9/40C12P19/04C12R1/07
CPCC12N9/2465C12N9/2468C12P19/04C12Y302/01081C12Y302/01158C12N1/205C12R2001/07
Inventor 李莉莉秦松武敏刘正一焦绪栋崔玉琳
Owner YANTAI INST OF COASTAL ZONE RES CHINESE ACAD OF SCI
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