Cat omega interferon mutant as well as preparation method and application thereof

A technology of interferon and mutant, which can be used in the preparation of medicines or reagents for preventing or treating feline viral diseases, the preparation and application of cat ω interferon, and the field of cat ω interferon mutants, which can solve the problem of weak antiviral activity, The problem of low expression of cat interferon
CN104788554AActive Publication Date: 2015-07-22THE INST OF BIOTECHNOLOGY OF THE CHINESE ACAD OF AGRI SCI
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
THE INST OF BIOTECHNOLOGY OF THE CHINESE ACAD OF AGRI SCI
Publication Date
2015-07-22

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Abstract

The invention discloses a cat omega interferon mutant as well as a preparation method and an application thereof, and belongs to the field of preparation and application of cat omega interferons. A cat omega 2 or omega 11 interferon mutant is obtained by comparing gene sequences and amino acid sequences of 13 subtypes of cat omega interferon and performing amino acid site-directed mutation on cat omega 2 or omega 11 interferon. The invention further discloses the method for preparing the cat omega interferon mutant. The method comprises the following steps: cloning a gene for coding the cat omega 2 or omega 11 interferon mutant into a baculovirus transfer vector, recombining with a baculovirus for infecting an insect host, expressing an exogenous gene, and obtaining a cat omega interferon protein expression product. The method is simple in process, the cat omega interferon capable of being safe to use can be efficiently and stably obtained, and the antiviral activity of the cat omega interferon mutant is remarkably improved. The cat omega 2 or omega 11 interferon mutant can be used for preparing drugs or reagents for preventing or treating cat viral diseases.
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Description

technical field

[0001] The present invention relates to feline ω interferon, in particular to a cat ω interferon mutant, and the present invention also relates to a preparation method of the feline ω interferon mutant and its use in the preparation of drugs or reagents for preventing or treating feline viral diseases The invention belongs to the field of preparation and application of feline omega interferon. Background technique

[0002] Interferon (IFN) is a highly biologically active glycoprotein produced by cells under the action of specific inducers, and has broad-spectrum antiviral activity in animal cells. It is currently known that interferon does not directly kill the virus, but interferes with the gene transcription of the virus or the translation of viral protein components by inducing the host's own cells to produce a variety of enzymes. Interferon can be divided into type I and type II IFN according to different cell sources, physical and chemical properties an...

Claims

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