Magnetic micro-particle chemiluminiscence detection kit for determining content of human Golgi body protein

Abstract

The invention relates to a magnetic micro-particle chemiluminiscence detection kit for determining the content of Golgi body protein of a human body. The kit comprises a Golgi body protein R1 reagent,a Golgi body protein R2 reagent, a magnetic separation reagent, a calibrator solution series and a chemiluminiscence substrate solution, wherein the Golgi body protein R1 reagent is an anti-Golgi body protein mouse monoclonal antibody diluent marked by fluorescein isothiocyanate, the Golgi body protein R2 reagent is an alkaline phosphatase marked Golgi body protein polyclonal antibody diluent, the magnetic separation reagent is a magnetic micro-particle diluent coated by an anti-fluorescein isothiocyanate mouse monoclonal antibody, the Golgi body protein calibrator solution is composed of a synthesized Golgi body protein antigen and a buffer solution, and the luminescent substrate solution is a Tris-HCl buffer solution which is catalyzed by alkaline phosphatase and contains dioxacycloethane. According to the invention, the signal intensity and sensitivity of immune response are greatly improved, and a more accurate, accurate, convenient, rapid and simple method is provided for the detection of the Golgi body protein.

Description

technical field

[0001] The invention relates to the field of medical diagnostic reagents, in particular to a magnetic particle chemiluminescence detection kit for detecting human Golgi body protein content using magnetic particle chemiluminescence technology and antigen-antibody combination technology. Background technique

[0002] Golgi protein (GP73) is a transmembrane Golgi glycoprotein including 400 amino acid residues, located in the 9q21.33 chromosome genome, also known as GOLM1 or GOLPH2. GP73 is composed of inner and outer C-terminals and hydrophobic N-3, and has a complete transmembrane structure. In normal people, GP73 is only expressed in gallbladder epithelial cells, almost not expressed in liver cells, but has a higher expression level in bile duct epithelial cells. When the liver is inflamed, the level of GP73 in liver cells increases, and the expression level of GP73 in liver cancer cells increases significantly. Relevant data show that the regulation mechan...

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