Diagnostic kit for in-vitro quantitative determination of GP73 (Golgi Protein 73) with latex-enhanced immunoturbidimetry

An immunoturbidimetric and latex-enhancing technology, applied in the field of medical immunology in vitro diagnosis, can solve the problems of unusable ELISA kits, labor-intensive and other problems, and achieve the effects of low cost, simple and rapid use, and high titer.
CN105223365AInactive Publication Date: 2016-01-06ENZYMAKER LABCHANGZHOU CO LTD
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
ENZYMAKER LABCHANGZHOU CO LTD
Publication Date
2016-01-06
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention relates to a kit for determination of human GP73 (Golgi Protein 73) content with a latex-enhanced immunoturbidimetry. The kit comprises a GP73 R1 reagent, a GP73 R2 reagent and a GP73 calibrator, wherein the GP73 R1 reagent comprises an electrolyte, a coagulant, a stabilizer, a surfactant, a preservative and a buffer solution; the GP73 R2 reagent comprises human GP73 resistant polyclonal antibody coated latex particles, an electrolyte, a stabilizer, a surfactant, a preservative and a buffer solution; the GP73 calibrator comprises a preservative, an electrolyte, a stabilizer, a GP73 antigen and a buffer solution. The kit is high in specificity, high in sensitivity, uniform, stable, rapid, convenient and applicable to various biochemical analyzers.
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Description

technical field

[0001] The invention relates to a kit for measuring the content of GP73 (Golgi glycoprotein-73, GolgiProtein73) in human serum by using a latex-enhanced immune turbidimetric method, which belongs to the field of in vitro medical immunodiagnosis. Background technique

[0002] GP73 (Golgi Glycoprotein-73, GolgiProtein73) is also known as type II Golgi membrane protein, Type II Golgimembraneprotein, Golph2), because Golph2 polyclonal antiserum can specifically recognize protein molecules with a size of 7.3×104 in human epithelial cell lysates, Therefore, this protein is also called GP73. GP73 is a transmembrane protein present in the Golgi apparatus with a molecular weight of 45KDa. However, GP73 undergoes a large amount of glycosylation in serum, and its molecular weight is about 73KDa in serum detection. Since GP73 is rich in acidic amino acids (5.5% aspartic acid, 12.2% glutamic acid), its isoelectric point PI = 4.72. GP73 is strongly hydrophilic but has hy...

Claims

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