AFP and GP73 combined detection kit

A detection kit, the technology of -73GP73, is applied in the field of AFP, GP73 combined detection kits and their preparation, which can solve problems such as false negatives, and achieve the effects of high specificity, accurate results and little hemolytic interference.

Active Publication Date: 2015-02-18
ZYBIO INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Therefore, the use of serum AFP as a marker of HCC has a certain degree of false positives and false negatives

Method used

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  • AFP and GP73 combined detection kit
  • AFP and GP73 combined detection kit

Examples

Experimental program
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Effect test

Embodiment 1

[0047] Preparation of test paper card of the present invention:

[0048] 1) Use pretreatment buffer to pretreat the gold pad. The pretreatment buffer is: stachyose 2g / L, alum 0.5g / L, sodium fructose diphosphate 1.5g / L, sodium hexametaphosphate 0.3g / L L, an aqueous solution of glycine 1.88g / L, pH=7.4, the specific steps of pretreatment are: soak the gold pad in the pretreatment solution for 2h, take it out and dry it at 37°C; Protein AFP antibody and Golgi glycoprotein-73GP73 antibody solutions were sprayed with their corresponding pretreated gold-labeled pads, respectively, to obtain gold-labeled pads containing alpha-fetoprotein AFP antibody and Golgi glycoprotein-73GP73 antibody respectively; fluorescence in the solution The mass ratio of microspheres to antibody is 5:1, the concentration of the solution is 10mg / ml, and the spraying amount is 4ul / cm;

[0049] 2) Spray alpha-fetoprotein AFP antibody and goat anti-mouse antibody on the detection line and quality control line ...

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Abstract

The invention relates to the field of biological detection, and particularly relates to a detection kit for quantitatively detecting alpha fetoprotein (AFP) and Golgi protein-73 (GP-73), a preparation method and application thereof. The kit comprises an AFP detection test paper card and a GP-73 detection test paper card which are independent from each other, wherein each detection test paper card comprises a bottom plate, as well as a sample pad, a gold mark pad, a nitrocellulose film and a water absorption pad which are arranged on the surface of the bottom plate and are sequentially arranged from a feeding end. The kit detects AFP and GP73 by using a fluorescent microspherev immunochromatography technology for the first time, has sensitivity and specificity, has the advantages of quick and simple operation, accurate result and the like, and is economical and practical.

Description

technical field [0001] The invention relates to the field of biological detection, in particular to an AFP and GP73 combined detection kit and a preparation method and application thereof. Background technique [0002] Alpha fetoprotein (AFP) is a glycoprotein synthesized by the liver and yolk sac in the early stage of fetal development. With the growth of fetal development and postnatal age, the level of AFP in the blood gradually decreases. The reference interval of adult serum AFP concentration 1-20μg / L. AFP is a sensitive and specific marker for hepatocellular carcinoma (HCC). Serum AFP concentrations exceeding 400 μg / L for 4 weeks or 200-400 μg / L for more than 5 weeks are highly suspected of hepatocellular carcinoma; at the same time, serum AFP levels also There are available liver cancer size judgment, efficacy evaluation and prognosis judgment of liver cancer. However, according to statistics, about 20%-30% of patients with hepatocellular carcinoma have low serum AF...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68
CPCG01N33/533
Inventor 潘能科
Owner ZYBIO INC
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