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Electrochemical/colorimetric dual-mode GP73 detection method based on H-rGO-Mn3O4 nano-enzyme

A nano-enzyme, electrochemical technology, applied in the direction of color/spectral property measurement, measurement device, scientific instrument, etc., can solve the problems of increasing production cost, improve accuracy and reliability, excellent electrical conductivity, reduce false negative or The effect of the probability of false positive detections

Inactive Publication Date: 2022-07-29
GUANGDONG UNIV OF PETROCHEMICAL TECH
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  • Application Information

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Problems solved by technology

The invention patent with the publication number CN 113533734 A relates to a method for detecting GP73 with a kit prepared based on a magnetic particle chemiluminescence method platform, but this method uses alkaline phosphatase to label the antibody, which increases the production cost

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  • Electrochemical/colorimetric dual-mode GP73 detection method based on H-rGO-Mn3O4 nano-enzyme
  • Electrochemical/colorimetric dual-mode GP73 detection method based on H-rGO-Mn3O4 nano-enzyme
  • Electrochemical/colorimetric dual-mode GP73 detection method based on H-rGO-Mn3O4 nano-enzyme

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[0046] The present invention will be described in detail below with reference to the accompanying drawings and specific embodiments.

[0047] figure 1 is based on H-rGO-Mn 3 O 4 Schematic of the nanozyme-based colorimetric / electrochemical aptamer sensor for dual-mode detection of GP73. Preparation of H-rGO-Mn with peroxidase-like catalytic activity by a stepwise synthesis 3 O 4 Nanozyme, via -NH on Apt 2 and H-rGO-Mn 3 O 4 -COOH on to form amide bond, and π-π bond to form H-rGO-Mn 3 O 4 -Apt detection probe; using layer-by-layer self-assembly technology, Au NPs were modified on the activated SPE to enhance the conductivity of the sensing interface; cDNA was modified on Au NPs / SPE to form a sensing interface; then H-rGO -Mn 3 O 4 -Apt detection probe was modified on the surface of cDNA / Au NPs / SPE, cDNA and Apt formed a double helix structure; finally, GP73 protein was modified on H-rGO-Mn 3 O 4 -Apt / cDNA / AuNPs / SPE surface to form a working electrode; at the same ti...

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Abstract

The invention provides a method for detecting Golgi protein 73 (GP73) by using an electrochemical / colorimetric dual-mode sensor based on H-rGO-Mn3O4 nano-enzyme, which comprises the following steps: modifying a screen-printed electrode by using gold nanoparticles (Au NPs), and fixing a complementary chain (cDNA) of a GP73 aptamer (Apt) on the surface of the electrode; the Apt is fixed on the surface of the H-rGO-Mn3O4 nano enzyme to serve as a detection probe; the electrochemical / colorimetric dual-mode aptamer sensor based on the H-rGO-Mn3O4 nano-enzyme is constructed by utilizing the competitive effect between cDNA and a detection probe and between GP73 and the detection probe, adopting 3, 3 ', 5, 5'-tetramethyl benzidine (TMB) as an electrochemical and colorimetric dual-function probe and utilizing a nano-enzyme catalytic signal amplification mode, dual-signal detection of GP73 is realized, and the lowest detection limit is 0.1 pg / mL.

Description

technical field [0001] The invention belongs to the field of biological detection, in particular to a method for detecting GP73 based on a composite nano-enzyme material. Background technique [0002] Golgi protein 73 (GP73) is a transmembrane glycoprotein present in the Golgi apparatus with a relative molecular weight of 73kDa. The commonly used method for GP73 detection is enzyme-linked immunosorbent assay (ELISA). The invention patent with publication number CN 111378627 A relates to a method for detecting GP73 in serum using a kit of monoclonal antibodies, but the kit used in this method has a long operation time, at least 40 hours, and the storage conditions of monoclonal antibodies are harsh, requiring specific storage environment. The invention patent with publication number CN 113533734 A relates to a method for detecting GP73 based on a kit prepared by a magnetic particle chemiluminescence method platform, but the method uses alkaline phosphatase to label the anti...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/327G01N21/01G01N21/33
CPCG01N27/3278G01N21/01G01N21/33
Inventor 李桂银陈敏王超贤周治德
Owner GUANGDONG UNIV OF PETROCHEMICAL TECH
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