269 results about "Benzidines" patented technology

The invention discloses bovine serum albumin-platinum composite nanomaterial mimetic peroxidase as well as a preparation method thereof and application. Bovine serum albumin is used as a template, and the bovine serum albumin-platinum composite nanomaterial mimetic peroxidase is prepared through biomineralization. Bovine serum albumin-platinum composite nanomaterials are prepared through the following method that chloroplatinic acid aqueous solutions are added to bovine serum albumin aqueous solutions and are mixed, sodium hydroxide aqueous solutions are added to obtain mixed solutions, and water bath heating is carried out; ultrafiltration is carried out on the solutions, then the solutions are washed, and bovine serum albumin-platinum composite nanomaterial aqueous solutions are obtained. The bovine serum albumin-platinum composite nanomaterials have excellent peroxidase activity, and can catalyze hydrogen peroxide oxidation 3, 3', 5, 5'-tetramethyl benzidine hydrochloride to be in color development. Meanwhile, the mimetic peroxidase resists acid and base, high temperature and high salinity, and has excellent short-term indoor temperature stability and long-term indoor temperature stability.

A single reagent system and a method to detect and measure oxidizing adulterants in bodily fluid being screened for drugs of abuse are disclosed. The system comprising a strip containing 0.05 to 0.2 micromole/25 sq. mm. of a benzidine derivative and is used to detect sodium hypochlorite (bleach), chlorine, hydrogen peroxide, sodium bromide, sodium iodide, sodium nitrite, and pyridinium chlorochromate adulterants in urine, sweat, saliva, blood or other bodily fluids during screening for drugs of abuse.

An organic light-emitting diode device (OLED) comprises a cathode, a light-emitting layer, and an anode in that order, in which there is located a first layer (L1) adjacent to the light-emitting layer on the anode side and a second layer (L2) adjacent to L1 on the anode side, in which:

(a) layer L1 comprises a benzidine derivative (B1) having an oxidation potential of 0.8-0.9 V; and

(b) layer L2 comprises a benzidine derivative (B2) having an oxidation potential greater than 0.7 V and exhibiting a glass transition temperature, Tg, of greater than 125° C.

The invention relates to a flexible transparent medlin membrane material and a relative preparation method, wherein the invention polymerizes 4, 4'-bis(3', 4'-dicarboxyphenoxy), 2, 2'-bis(trifluoromethyl)-4, 4'-benzidine, 3, 3'-diaminodiphenylsulfone dapsone and 3, 3'-dimethyl-4, 4'-diamino diphenyl methane in polar solvent to obtain medlin, uses tape casting method to treat the medlin on a clean glass plate, bakes in a membrane bake oven at 80DEG C for 2h until the solvent is volatilize and the membrane is solidified, moves the glass plate coated with a medlin membrane into an oven, increases the oven temperature from room temperature to 350DEG C at the heating rate of 2DEG C/min, naturally cools to room temperature, takes out the glass plate coated with one medlin membrane from the oven, arranges the glass plate into water, boils water to remove the medlin membrane from the glass plate, to obtain flexible transparent medlin membrane material. The ultraviolet cut-off wavelength of the medlin membrane is 395-430nm, and the glassivation transformation temperature is 254-280DEG C.

The invention discloses a ZnCuInS/ZnS quantum dot white light LED (Light Emitting Diode) based on compensation light emitting and a manufacturing method thereof. The LED is in a dual-layer light-emitting structure and comprises a glass substrate, a transparent ITO (Indium Tin Oxide) electrode deposited on the glass substrate, a PEDOT:PPS(Poly(3,4-ethylenedioxy Thiophene):(Poly(4-styrenesulfonate)) layer which is formed on the ITO electrode by spin coating, a Poly-TPD (N,N'-bis(4-butylpheny)-N,N'-bis(phenyl) benzidine) layer which is formed on the PEDOT:PPS layer by spin coating to generate blue and green light, a ZnCuInS/ZnS quantum dot layer which is formed on the Poly-TPD layer to generate red light as well as an Al electrode, wherein the PEDOT:PPS layer is a buffer layer; the ITO electrode is transparent and is used as a positive electrode of the LED; the Poly-TPD layer generates the blue and green light; and the ZnCuInS/ZnS quantum dot layer generates red light which is complemented with the blue and green light which are generated by the Poly-TPD layer to form white light. According to the ZnCuInS/ZnS quantum dot white light LED disclosed by the invention, a non-toxic metallic ZnCuInS/ZnS quantum dot is used as a main light-emitting layer, and thus the requirement of environment protection is met; and the ZnCuInS/ZnS quantum dot white light LED has the advantages of favorable color rendering property, simple preparation process, low cost and the like.

The invention discloses a folic acid-porous platinum-graphene oxide composite nano material as well as an application thereof in detecting tumor cells. The folic acid-porous platinum-graphene oxide composite nano material is prepared by the following steps: by taking graphene oxide as a substrate, carrying out amidation to crosslinked folic acid molecules; and carrying out in-situ synthesizing on platinum nano particles with porous structures. The folic acid-porous platinum-graphene oxide composite nano material can specifically identify a folate receptor on the surface of the cell, and meanwhile has a characteristic of simulating peroxidase and can catalyze hydrogen peroxide oxidized 3, 3', 5, 5'-tetramethyl benzidine hydrochloride to develop color. The number of the tumor cells can be quickly and agilely detected by means of chromogenic reaction, and 30 MCF-7 (Michigan Cancer Foundation-7) cells can be detected to the minimum extent. The material further can catalyze diaminobenzidine to generate a reddish brown precipitate on the surface of the cell so as to realize targeted positioning detection.

The invention belongs to the field of detecting a trace amount of ions in the water environment, and particularly relates to a visualization method for rapidly detecting a trace amount of uranyl ions in the water environment. The method mainly includes the steps that DNAzyme with the specific recognition function on UO2 <2+> is fixed to the surfaces of magnetic beads, and horse radish peroxidase is preassembled on the surface of nano-gold; then the magnetic beads are connected with the nano-gold through the cutting effect of the UO2<2+> on the DNAzyme and the hybridization reaction of DNA, after separation and collection are carried out through an external magnetic field, H2O2 oxidation tetramethyl benzidine is efficiently catalyzed through the horse radish peroxidase to enable a solution to be changed from the blank to the blue, and therefore sensitive and specific visualization rapid detection of the UO2<2+> ions is achieved. As the method has the advantages of being high in sensitivity, high in specificity, high in matrix interference resistance, simple, rapid, low in cost and the like, the method can be used for site rapid visualization detection of the trace amount of UO2<2+> ions in various water samples.

The invention discloses preparation and application of a covalent organic framework porous nano composite material, and belongs to the technical field of environmental protection. Firstly, 2,4,6-trialdehyde phloroglucinol (Tp) and benzidine (BD) are subjected to a Schiff base reaction under proper conditions to prepare a covalent organic framework (COF-TpBD), then diindium trisulfide (In2S3) nanoparticles are loaded on the COF-TpBD in situ, and the composite material (In2S3@COF) with In2S3 and COF characteristics is obtained. The In2S3@COF prepared by the method is rich in a large amount of Selement and can adsorb Hg2+ through HgS interaction, and on the other hand, the inherent large specific surface area and excellent pore size of the COF can also greatly improve the adsorption capacityto Hg2+. Inductively coupled plasma mass spectrometry test results show that the In2S3@COF has excellent Hg2+ adsorption performance. The method is simple, stable in structure, low in cost, environmentally friendly and high in adsorption and removal efficiency of Hg 2+ in a water body, and can be used as an efficient adsorbent for Hg 2+ in environmental wastewater.

The invention provides a CsxWO3 type peroxide mimic enzyme, and a preparation method and application thereof. The CsxWO3 type peroxide mimic enzyme has the activity similar to that of peroxidase, and can be used for catalyzing H2O2 to generate a great quantity of hydroxyl free radicals and effectively oxidizing a substrate, 3,3',5,5'-tetramethyl benzidine to generate a color developing reaction. The CsxWO3 type peroxide mimic enzyme is combined with glucose oxidase for use, the content of glucose in a solution can be indirectly detected by measuring an ultraviolet absorption value of the oxidized 3,3',5,5'-tetramethyl benzidine and the detection limit is 0.1 micro milliliter. Compared with horse radish peroxidase (HRP), the CsxWO3 nano material has the advantages of simple preparation process, low cost, stable chemical activity and the like; the catalytic effect and the applicability of the CsxWO3 nano material are higher than those of natural peroxidase, and thus the CsxWO3 nano material can be used as a substitute of natural peroxidase.

The invention discloses a preparation method of a fluorine-containing branched sulfonated polyimide proton conducting membrane. The preparation method is characterized by comprising the following steps: under protection of nitrogen gas, adding m-cresol, 1,4,5,8-naphthalene tetracarboxylic dianhydride and benzoic acid into a reactor, and stirring until solid substances are dissolved; adding m-cresol, 2,2'-disulfonic acid benzidine and triethylamine into a container, stirring until the solid substances are dissolved, then adding 1,3,5-tri(2-trifluoromethyl-4-aminophenoxy)benzene, 4,4'-diaminodiphenyl ether to stir to dissolve to obtain a mixture; putting the mixture in a constant-pressure dropping device, and dropwise adding the mixture into a reactor, stirring and heating for 15-24 hours at temperature of 40-100 DEG C; then casting a reacted material into a membrane, and drying the membrane; then soaking and washing to prepare the fluorine-containing branched sulfonated polyimide proton conducting membrane. The prepared fluorine-containing branched sulfonated polyimide proton conducting membrane is good in performance, and is suitable for the fields of all-vanadium redox flow cells, hydrogen oxygen fuel cells, direct methanol fuel cells and the like.

The invention discloses a processing method of benzidine manufacturing wastewater and resource recycling method, which comprises the following steps: adjusting pH value of benzidine manufacturing wastewater to 4-7; adsorbing through first composite functional resin; recycling o-chloroaniline; adjusting adsorbed water to 7-10; adsorbing through second composite functional resin; reducing COD below 500mg/L and o-chloroaniline below 5mg/L; adsorbing first composite functional resin through HCl solution completely; obtaining high-density desorption liquid; neutralizing through NaOH solution; separating; recycling o-chloroaniline with density over 98%; regenerating the second composite functional resin through the same method; allocating the first resin through high-density desorption liquid; reducing the discharge quantity of organic pollution.

The invention discloses a simple and low-cost silver ion visual quantitative detection method. The method adopts an organic solvent which can be mutually soluble with water and has density more than 1g/mL at the temperature of 25 DEG C for dissolving 3,3',5'5'-tetramethyl benzidine (TMB). When aqueous solution containing silver ions is dropwise added into TMB solution, Ag<+> quickly oxidizes TMB to generate blue quinoid benzidine. As the adopted organic solvent can be taken as a bicolour layering intelligent regulator, a unique bicolour mixed solution which is blue at the upper layer and colourless at the lower layer is obtained finally; meanwhile, the length of the blue solution is positively correlated with concentration of Ag<+>, so that quantitative detection on Ag<+> at micromole level can be realized by visualizing and observing with naked eyes and measuring the length of the blue solution. According to the method disclosed by the invention, a Ag<+> detection process is extremely simple, an operator not trained professionally can carry out an experiment, more importantly, a quantitative signal can be read without using instrument or equipment, and on-site analysis and real-time detection on a sample can be realized while analysis cost is greatly reduced.

The invention discloses tungsten-oxide-quantum-dot-material simulated peroxidase. A tungsten oxide quantum dot material has excellent peroxidase activity and can catalyze color developing agents such as hydrogen-peroxide-oxidized 3,3',5,5'-tetramethyl benzidine hydrochloride, diammonium 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonate) and o-phenylenediamine to develop colors. In this way, a novel simulated peroxidase inorganic nano-material capable of replacing natural enzymes is provided. The tungsten oxide quantum dot material is prepared in the mode that tungsten sulfide serves as a precursor, and reacting is carried out through a simple ultrasonic step and a hydrothermal method. The material is simple in synthesis step, environmentally friendly and low in cost; in addition, the peroxidase simulating effect is obvious, and therefore good application prospects are achieved in novel catalytic oxidation and medical analysis.

The invention belongs to the field of printing technology, and particularly relates to cable lettering ink which is characterized by comprising the following raw materials in parts by weight: 4-6 parts of polyvinyl chloride resin, 5-9 parts of acrylate polymer, 30-40 parts of methyl ethyl ketone, 13-18 parts of toluene, 5-10 parts of isobutyl methacrylate, 10-18 parts of tetrahydrofuran, 3-5 parts of benzidine yellow or titanium dioxide or lithopone with fineness of 400-800 meshes, 6-10 parts of 2-lead ethylcaproate, 0.3-0.5 part of methylparaben or propylparaben or butylparaben and 3-4.5 parts of butyl acetate. The invention also discloses a preparation method of the lettering ink. The cable lettering ink and the preparation method provided by the invention have the main beneficial effects that the preparation method is simple and easy to master, and the qualified rate of the finished product is high; the lettering ink has the advantages of high adhesion, high drying speed, bright luster and longer shelf life.

The invention provides a reagent for accelerating Fenton reaction as well as a preparation method and application of the reagent. The reagent for accelerating the Fenton reaction is copper carbon dot CuCD, and the preparation method comprises the following steps: S1, mixing 3, 4-dihydroxyphenylpropionic acid and copper chloride, dissolving the mixture with deionized water, uniformly stirring, adding 2, 2'-(ethylenedioxy) diethylamine, and performing stirring; S2, transferring the solution obtained in the step S1 into a reaction kettle, reacting at 170-190 DEG C for 4-6 hours, and performing cooling to room temperature; S3, centrifuging the solution after the reaction in the step S2, enabling the solution to pass through a water-phase filter membrane, then putting the filtrate into a dialysis bag for dialysis, and freeze-drying to obtain the copper-carbon dots CuCD. The reagent for accelerating the Fenton reaction can be combined with a Fenton reagent, is applied to treatment of organic matters in water, has good biological safety, can accelerate the reaction rate of the Fenton reagent, improves the degradation rate of pollutants in wastewater, and accelerates the oxidation rate of 3, 3', 5, 5'-tetramethyl benzidine.

The invention provides a hydrogen peroxide rapid detection method of platinum monatomic nano-enzyme and sterilization application, and belongs to the technical fields of nano-catalysis, environment detection, medical antibiosis and the like. According to the method, platinum monatomic nano-enzyme is taken as a catalyst, 3, 3 ', 5, 5'-tetramethyl benzidine is taken as a color developing agent, andenzyme-free color development detection of hydrogen peroxide is realized. In addition, good in-vitro and in-vivo wound sterilization is realized by utilizing the activity of the platinum monatomic nano-enzyme, so that the sterilization performance of H2O2 is greatly improved, the use concentration of H2O2 is reduced, and the toxic and side effects of H2O2 are avoided. The nano-enzyme material is expected to be widely applied to the fields of nano-catalysis, environmental detection, biomedicine and the like.

The invention discloses a mesoporous manganese oxide nano-enzyme with a spherical yolk-eggshell structure, and a preparation method and application thereof. Manganese-tannic acid-formaldehyde polymer spheres are roasted in an air atmosphere, so that the mesoporous manganese oxide nano-enzyme with the spherical yolk-eggshell structure is obtained. The mesoporous manganese oxide nano-enzyme is uniform in particle size distribution (70 nm), and has a high specific surface area (127 m<2>/g), a mesoporous structure (the aperture is 5.8 nm) and the yolk-eggshell structure. The mesoporous manganese oxide nano-enzyme with the spherical yolk-eggshell structure has oxidase-like activity and can catalytically oxidize 3, 3', 5, 5'-tetramethyl benzidine (TMB) to generate a color reaction, and the Michaelis constant (Km) of the material with TMB as a substrate is 97 mmol/L. The material also has oxidability, can be used for colorimetric detection of reduced biomolecules (such as glutathione, cysteine and ascorbic acid), and has good selectivity.

The invention discloses a benzidine derivative and an organic light-emitting diode thereof and relates to the technical field of organic optoelectronic materials. Aromatic amine nitrogen atoms on thebenzidine derivative disclosed by the invention contain lone pair electrons and show good electropositivity; the benzidine derivative disclosed by the invention has a larger conjugation system, thereby having higher hole mobility and showing better hole transport performance. In addition, a substituted group with large volume is also introduced into the benzidine derivative disclosed by the invention, so that the glass temperature and thermal stability of a material are effectively improved, and the material film formation is facilitated. The organic light-emitting diode comprises an anode, acathode and one or more organic matter layers; the organic matter layers are positioned between the anode and the cathode; at least one of the organic matter layers contains the benzidine derivative disclosed by the invention. The organic light-emitting diode disclosed by the invention has lower drive voltage, higher luminous efficiency and luminous brightness and longer service life.

The invention relates to a method for in-situ detecting Hg<2+> in a water sample through a photocatalysis visible manner. The method is characterized in that Hg<2+> is specifically combined with thymine (T) to form a -Hg<2+>-T structure, so that two T-enriched CpG-oligodeoxynucleotides are matched with each other in a staggering manner to form double strands. ethidium bromide (EB) can be embedded in the double strands, and 3,3',5,5'-tetramethyl benzidine (TMB) is oxidized under the radiation of a green LED (light emitting diode) lamp into blue. The semi-quantitative analysis of mercury ions can be realized by observing the color variation of the solution. By adopting the method, 0.05ngmL<-1> of Hg<2+> in the water sample can be detected through eyes, and the determination requirement of the Hg<2+> (1ngmL<-1>) in water samples such as drinking water, irrigation water for farmland and the like can be met. The method is good in selectivity, high in sensitivity, capable of avoiding the complicated separation and enriching process and directly applicable to the in-situ analysis of Hg<2+>.

The invention relates to the technical field of environmental functional materials, in particular to a three-dimensional graphene/COFs composite material and a preparation method and application thereof. The preparation method comprises the following steps: suspending three-dimensional graphene hydrogel in dimethyl sulfoxide, performing mixing with a dimethyl sulfoxide solution containing benzenetricarboxaldehyde and benzidine, dropwise adding glacial acetic acid, and stirring at normal temperature until a green flocculent composite material is generated. According to the method, the investment of energy consumption can be reduced, and the requirements of environmental protection and economic effects are met; and the three-dimensional graphene/COFs composite material prepared by the methodcan be applied to efficient enrichment and separation of organic phosphorus.

The invention discloses a method for quickly detecting the extracellular respiratory activity of microorganisms. The method comprises the following steps: adding color developing liquid containing tetramethyl benzidine and hydrogen peroxide into microbial suspension liquid for fully producing a color developing reaction; adding a sulfuric acid solution for stopping the color developing reaction, changing blue to yellow, and detecting the OD 450 nm value by an enzyme-linked immunosorbent assay detection instrument, wherein the OD value is the extracellular respiratory activity value of a microorganism sample. The method is simple and quick to carry out, a detection result can be most quickly obtained within 3 minutes, the method can be used for quickly detecting the microorganism sample and is very good in specificity, and the detection result is hardly influenced by other common microorganisms.

The invention discloses a kit used for detecting double resistance of aspirin and clopidogrel drugs. The kit comprises (1) an assay plate coated by CK-MB (creatine kinase-MB) antibodies; (2), an enzyme labeled CK-MB specific antibody adopting horse radish peroxidase labelling; (3), a CK-MB standard solution; (4), a substrate developing solution adopting tetramethyl benzidine; (5), a stop solution adopting a hydrochloric acid buffer solution; (6), a concentration washing solution adopting deionized water; and (7), a concentration reconstitution fluid adopting a phosphate buffer solution containing 0.1%-5% of bovine serum albumin. The kit detecting sample adopts serum and can be preserved for a long time, the detecting process is simple and rapid, and the accuracy of the kit can be higher than 95%.