Method for in-situ detecting Hg<2+> in water sample through photocatalysis visible manner
An on-site detection and photocatalysis technology is applied in the direction of analyzing materials through chemical reactions and analyzing materials through observation of the influence of chemical indicators. It can solve problems such as low sensitivity and excessive Hg2+ content, and achieve high sensitivity. , the effect of simple method
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Embodiment 1
[0019] Sequentially add 10 μL to a clean culture plate at a concentration of 10 μmol L -1 DNA solution (sequence 5'-CGCATTCAGGATTCTCTACTCGTA-3', 5'-TTCGTGTTGTGTTTCCTGTTTGCG-3'), 1mL of different concentrations of Hg 2+ Standard solution / environmental water sample, 100 μL concentration is 2 mmol L -1 Ethidium bromide solution (final concentration 100 μM), 0.89 mL of 0.2 mol L -1 PBS buffer solution (pH=4.0), incubate at room temperature in the dark for 15 min; then, add 40 μL of 10 mg mL to the mixed solution -1 The TMB solution was irradiated with a green LED light for 15 minutes, and the color of TMB was photocatalyzed and oxidized; the color of Hg in the water sample was obtained by comparing the color of the environmental water sample with the standard series. 2+ Concentration range, to determine the Hg in environmental water samples 2+ Whether it exceeds the national standard value.
[0020]
Embodiment 2
[0022] Add 10 μL of 10 μM DNA solution (sequence 5’-CGCATTCAGGATTCTCTACTCGTA-3’, 5’-TTCGTGTTGTGTTTCCTGTTTGCG-3’) and 1 mL of Hg 2+ Standard solution / environmental water sample, 0.89 mL with a concentration of 0.2 mol L -1 PBS buffer solution (pH=4.5), 100 μL of 4 mM ethidium bromide solution (final concentration of 50 μM), incubate at room temperature for 30 min in the dark; add 40 μL of 10 mg mL -1 The TMB solution in the water sample was irradiated with a green LED light for 30 min; the color of the Hg in the water sample was obtained by comparing the color of the environmental water sample with the standard series. 2+ Concentration range, to determine the Hg in environmental water samples 2+ Whether it exceeds the national standard value.
[0023]
[0024] Using Examples 1 and 2 to measure Hg in drinking water, tap water, irrigation water, and standard water samples (GSBZ 50016-90) 2+ The concentration is shown in Table 1, which is in good agreement with the me...
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