Bovine serum albumin-platinum composite nanomaterial mimetic peroxidase

A technology of bovine serum albumin and composite nanomaterials, which is applied in the field of nanotechnology and bionics, can solve the problems of limited natural enzyme sources, loss of natural enzyme activity, harsh operating conditions, etc., and achieves good stability, excellent stability, and a preparation method Easy and fast effect
CN103433484AInactive Publication Date: 2013-12-11FUJIAN MEDICAL UNIV
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
FUJIAN MEDICAL UNIV
Publication Date
2013-12-11
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses bovine serum albumin-platinum composite nanomaterial mimetic peroxidase as well as a preparation method thereof and application. Bovine serum albumin is used as a template, and the bovine serum albumin-platinum composite nanomaterial mimetic peroxidase is prepared through biomineralization. Bovine serum albumin-platinum composite nanomaterials are prepared through the following method that chloroplatinic acid aqueous solutions are added to bovine serum albumin aqueous solutions and are mixed, sodium hydroxide aqueous solutions are added to obtain mixed solutions, and water bath heating is carried out; ultrafiltration is carried out on the solutions, then the solutions are washed, and bovine serum albumin-platinum composite nanomaterial aqueous solutions are obtained. The bovine serum albumin-platinum composite nanomaterials have excellent peroxidase activity, and can catalyze hydrogen peroxide oxidation 3, 3', 5, 5'-tetramethyl benzidine hydrochloride to be in color development. Meanwhile, the mimetic peroxidase resists acid and base, high temperature and high salinity, and has excellent short-term indoor temperature stability and long-term indoor temperature stability.
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Description

technical field

[0001] The invention relates to a bovine serum albumin-platinum composite nano material with the characteristic of simulating peroxidase, and belongs to the field of nano technology and bionic technology. Background technique

[0002] Enzymes are very important proteins with catalytic activity in organisms. They have high catalytic efficiency, strong specificity, and mild reaction conditions. The growth, development, reproduction and other life activities of organisms are inseparable from the catalysis of enzymes. However, the sources of natural enzymes are limited, difficult to purify, and expensive. At the same time, natural enzymes are easily affected by various physical and chemical factors and lose their activity. Therefore, in practical applications, the operating conditions of experiments are relatively harsh, which greatly limits their applications. In recent years, the research, development and application of artificial mimetic enzymes have attracte...

Claims

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