Hydrogen peroxide rapid detection method of platinum monatomic nano-enzyme and sterilization application
A hydrogen peroxide and detection method technology, applied in the field of environmental monitoring technology and medical antibacterial, enzyme engineering, can solve the problems of epidermal tissue adverse reactions, achieve good sterilization effect, prevent wound infection, and reduce the effect of using concentration
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Embodiment 1H2
[0020] Example 1H 2 O 2 detection of:
[0021] (1) Detection method
[0022] 0.1mg / mL platinum single-atom nanomaterials with 1mM TMB and 1mM H 2 O 2 Mix in acetate buffer (pH=4) for 20 min at room temperature. The absorbance of various solutions was measured in a cuvette excited at 652nm by UV-Vis absorption spectroscopy.
[0023] (2) Drawing of standard working curve:
[0024] In step (1), along with the H in the sample 2 O 2 The increase of TMB, the degree of oxidation of TMB is continuously enhanced, in the range of 5-500μM, the system 652nm absorbance intensity and H 2 O 2 The concentration has a good linear relationship, the linear correlation coefficient R 2 =0.99( figure 2 ).
Embodiment 2
[0026] Platinum single-atom nanozymes enhance H 2 O 2 Antibacterial performance investigation:
[0027] (1) Culture of Escherichia coli (E.coli)
[0028] E. coli was moved into LB liquid medium, and placed in a bacterial shaker at a rotation speed of 120 rpm at 37°C for 12 hours. Subsequently, the bacteria were collected by centrifugation at 10,000 rpm for 10 min and diluted to 10 with LB medium. 7 CFU mL -1 .
[0029] (2) Sterilization method
[0030] Set four groups of experiments as the control group (E.coli), E.coli+H 2 O 2 , E.coli+nanozyme, E.coli+nanozyme+H 2 O 2 group, where E.coli is 10 7 CFU, platinum single atom nanozyme 0.1mg / mL and H 2 O 2 After incubating and culturing for 6 hours under the condition of pH = 4, apply it to LB medium, culture at 37°C for 15 hours, and observe the growth of bacteria on the plate.
[0031] (3) Analysis of the results of sterilization
[0032] The bactericidal effect was inversely correlated with the number of plate col...
Embodiment 3
[0033] Embodiment 3 constructs artificial wound model, investigates the therapeutic effect of nanozyme:
[0034] (1) wound infection
[0035] The backs of experimental mice were depilated using depilatory cream. Next, use sterilized and sterilized surgical scissors to cut a wound on the back of the depilated mouse.
[0036] (2) trauma management
[0037] Mice with wounds on the back were divided into four groups, all coated with 10 7 CFU of E.coli, four groups of experiments were set up, which were control group, H 2 O 2 , nanozyme, nanozyme + H 2 O 2 group, platinum single-atom nanozyme 0.1mg / mL, H 2 O 2 It was 1 mM, applied to the wound under the neutral condition of PBS, and observed continuously for 6 days.
[0038] (3) Observe the wound recovery
[0039] from Figure 4 It can be seen that, compared with the other three groups, the nanozyme+H 2 O 2 The wounds treated in the group had obvious scabs during the treatment. More importantly, the H used in the expe...
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