Hydrogen peroxide rapid detection method of platinum monatomic nano-enzyme and sterilization application

A hydrogen peroxide and detection method technology, applied in the field of environmental monitoring technology and medical antibacterial, enzyme engineering, can solve the problems of epidermal tissue adverse reactions, achieve good sterilization effect, prevent wound infection, and reduce the effect of using concentration

Inactive Publication Date: 2020-05-08
DALIAN UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, excess H 2 o 2 (166mM-1M) can c...

Method used

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  • Hydrogen peroxide rapid detection method of platinum monatomic nano-enzyme and sterilization application
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  • Hydrogen peroxide rapid detection method of platinum monatomic nano-enzyme and sterilization application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1H2

[0020] Example 1H 2 O 2 detection of:

[0021] (1) Detection method

[0022] 0.1mg / mL platinum single-atom nanomaterials with 1mM TMB and 1mM H 2 O 2 Mix in acetate buffer (pH=4) for 20 min at room temperature. The absorbance of various solutions was measured in a cuvette excited at 652nm by UV-Vis absorption spectroscopy.

[0023] (2) Drawing of standard working curve:

[0024] In step (1), along with the H in the sample 2 O 2 The increase of TMB, the degree of oxidation of TMB is continuously enhanced, in the range of 5-500μM, the system 652nm absorbance intensity and H 2 O 2 The concentration has a good linear relationship, the linear correlation coefficient R 2 =0.99( figure 2 ).

Embodiment 2

[0026] Platinum single-atom nanozymes enhance H 2 O 2 Antibacterial performance investigation:

[0027] (1) Culture of Escherichia coli (E.coli)

[0028] E. coli was moved into LB liquid medium, and placed in a bacterial shaker at a rotation speed of 120 rpm at 37°C for 12 hours. Subsequently, the bacteria were collected by centrifugation at 10,000 rpm for 10 min and diluted to 10 with LB medium. 7 CFU mL -1 .

[0029] (2) Sterilization method

[0030] Set four groups of experiments as the control group (E.coli), E.coli+H 2 O 2 , E.coli+nanozyme, E.coli+nanozyme+H 2 O 2 group, where E.coli is 10 7 CFU, platinum single atom nanozyme 0.1mg / mL and H 2 O 2 After incubating and culturing for 6 hours under the condition of pH = 4, apply it to LB medium, culture at 37°C for 15 hours, and observe the growth of bacteria on the plate.

[0031] (3) Analysis of the results of sterilization

[0032] The bactericidal effect was inversely correlated with the number of plate col...

Embodiment 3

[0033] Embodiment 3 constructs artificial wound model, investigates the therapeutic effect of nanozyme:

[0034] (1) wound infection

[0035] The backs of experimental mice were depilated using depilatory cream. Next, use sterilized and sterilized surgical scissors to cut a wound on the back of the depilated mouse.

[0036] (2) trauma management

[0037] Mice with wounds on the back were divided into four groups, all coated with 10 7 CFU of E.coli, four groups of experiments were set up, which were control group, H 2 O 2 , nanozyme, nanozyme + H 2 O 2 group, platinum single-atom nanozyme 0.1mg / mL, H 2 O 2 It was 1 mM, applied to the wound under the neutral condition of PBS, and observed continuously for 6 days.

[0038] (3) Observe the wound recovery

[0039] from Figure 4 It can be seen that, compared with the other three groups, the nanozyme+H 2 O 2 The wounds treated in the group had obvious scabs during the treatment. More importantly, the H used in the expe...

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Abstract

The invention provides a hydrogen peroxide rapid detection method of platinum monatomic nano-enzyme and sterilization application, and belongs to the technical fields of nano-catalysis, environment detection, medical antibiosis and the like. According to the method, platinum monatomic nano-enzyme is taken as a catalyst, 3, 3 ', 5, 5'-tetramethyl benzidine is taken as a color developing agent, andenzyme-free color development detection of hydrogen peroxide is realized. In addition, good in-vitro and in-vivo wound sterilization is realized by utilizing the activity of the platinum monatomic nano-enzyme, so that the sterilization performance of H2O2 is greatly improved, the use concentration of H2O2 is reduced, and the toxic and side effects of H2O2 are avoided. The nano-enzyme material is expected to be widely applied to the fields of nano-catalysis, environmental detection, biomedicine and the like.

Description

technical field [0001] The invention relates to the fields of enzyme engineering, environmental monitoring technology and medical antibacterial technology, in particular to a rapid hydrogen peroxide detection and bactericidal application based on a platinum single-atom nanozyme. Background technique [0002] Natural enzymes have broad application prospects due to their high specificity and outstanding substrate efficiency. However, its inherent instability, limited resources and high cost of the process hinder its wide application. Nanozymes are a class of nanomaterials with natural enzyme functions. Compared with natural proteases, they have the advantages of low cost, strong biodegradation resistance, and large production volume. The application in biosensors and other fields has attracted more and more attention. Platinum is a rare noble metal material that has received extensive attention due to its high-temperature oxidation resistance and thermoelectric properties. S...

Claims

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Application Information

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IPC IPC(8): G01N21/33A61P31/04A61K33/40
CPCA61K33/40A61P31/04G01N21/33
Inventor 赵慧敏范耀方全燮
Owner DALIAN UNIV OF TECH
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