Golgi protein-73 mini-ELISA detection system and use method thereof

A Golgi protein and detection system technology, which is applied in the field of Golgi protein-73 small ELISA detection system, can solve the problems of limited application, cumbersome operation, and dependence on large detection instruments, and achieve the effect of low detection cost, simple and efficient operation
CN107436360AInactive Publication Date: 2017-12-05FOSHAN YETAI TECH
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
FOSHAN YETAI TECH
Publication Date
2017-12-05
Estimated Expiration
Not applicable · inactive patent

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Abstract

The present invention discloses a Golgi protein-73 mini-ELISA detection system comprising a kit, a portable fluorescence detector, a magnet plate and a magnetic bead carrier, wherein the kit comprises a sample diluent, a washing buffer, an antibody diluent, a Blocking buffer, a chromogenic reagent, a stop solution, a bead activator, a GP73 standard, Golgi protein-73 recognization monoclonal antibody 5F10 and horse radish peroxidase-labeled Golgi protein-73 recognization monoclonal antibody 5B12. The invention also discloses a using method of the detection system. The Golgi protein-73 mini-ELISA detection system does not require the use of a microplate reader, and can be used in relevant units being lack of large equipment. The system can be used to complete the entire testing process on the basis of an immunomagnetic bead serum GP73 double monoclonal antibody sandwich ELISA test kit by combination of a portable fluorescence detector, testing cost is low, and operation is simple and efficient.
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Description

technical field

[0001] The invention relates to an ELISA detection system, in particular to a small-sized ELISA detection system for Golgi protein-73 (GP73). At the same time, the invention also relates to a method for using the small ELISA detection system. Background technique

[0002] Liver cancer is one of the most common malignant tumors in the world, especially in my country, the incidence of liver cancer accounts for about 55% of the global total, and its mortality rate ranks second in the mortality rate of malignant tumors in my country. Because the early symptoms of liver cancer are not obvious, and it has the characteristics of rapid development and early metastasis, it is difficult to diagnose early clinically and the prognosis is poor. At present, B-ultrasound and serological detection of alpha-fetoprotein (AFP) content are the main means of liver cancer screening. However, B-ultrasound is difficult to distinguish small liver cancer and cirrhotic nodules, and A...

Claims

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