Preparation method of active collagen with triple-helix structure

Abstract

The invention relates to a preparation method of collagen; fresh pigskin is used as a raw material; the raw material is treated by pretreatment, fat removal, and acid-enzyme treatment; ultrasonic treatment is employed to further improve extraction efficiency; and finally purification is performed to prepare a collagen product with high purity. The preparation method of collagen has high extraction efficiency, and the product purity is up to above 98%. The product is active collagen with a maintained triple-helix structure, has low immunogenicity and high biocompatibility, is biodegradable, and is applicable to medical purposes.

Description

technical field

[0001] The invention relates to a preparation method of active collagen with a triple helix structure, which belongs to the field of protein engineering. The product is active collagen maintaining a triple helix structure, has low immunogenicity, high biocompatibility, is biodegradable, and is suitable for use in medical use. Background technique

[0002] Collagen is a group of hard proteins with the most abundant content and widest distribution in vertebrates. 25%-33% of the total protein content of the animal body. Several types of collagen are often contained in the same tissue, and a certain type is often the main one. Type I collagen is distributed in all parts of the body, mainly in the skin, tendons and ligaments, and has strong tensile strength. Type II collagen mainly exists in hyaline cartilage and vitreous body, and has a strong ability to resist pressure. Type III collagen is widely distributed in tissues with high extensibility, such as loose...