Biological membrane and preparation method thereof

A biomembrane and membrane tissue technology, applied in tissue regeneration, medical science, prosthesis, etc., can solve the problem of high toxicity of polyethylene glycol octylphenyl ether, poor biocompatibility and safety, damage to collagen fiber structure, etc. problem, to achieve good decellularization and degreasing effect, improve decellularization efficiency, and less damage to collagen fibers
CN105963782AInactive Publication Date: 2016-09-28SHAANXI RUISHENG BIOTECH
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
SHAANXI RUISHENG BIOTECH
Publication Date
2016-09-28
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention relates to the technical field of tissue engineering, in particular to a biofilm and a preparation method thereof. This method has simple process, low cost, less damage to the collagen fibers of membrane tissue, and good decellularization effect. The obtained biofilm retains the natural double-layer structure of animal membrane tissue and can guide tissue regeneration. The clinical application of the membrane has created conditions. The embodiment of the present invention provides a method for preparing a biofilm, comprising: step 1) removing the fatty tissue on the surface of the mammalian membrane tissue to obtain the membrane tissue after the preliminary degreasing; step 2) treating the membrane tissue after the preliminary degreasing with alkali solution soaking for decellularization to obtain decellularized membrane tissue; step 3) soaking the decellularized membrane tissue in an organic solvent, and performing degreasing treatment under vibration to obtain degreased membrane tissue; step 4) degreasing The final membrane tissue was flattened, dried, and sterilized to obtain a biofilm.
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Description

technical field

[0001] The invention relates to the technical field of guided tissue regeneration, in particular to a biofilm and a preparation method thereof. Background technique

[0002] Guided tissue regeneration is an ideal technique to promote periodontal new attachment; this technique is to cover the periodontal tissue defect with a guided tissue regeneration film to prevent fast-growing epithelial cells and fibroblasts from growing into the defect area. Thereby providing a certain space for the growth of periodontal ligament cells and bone cells and promoting the regeneration of periodontal tissue. Therefore, an ideal guided tissue regeneration membrane should have a double-layer structure with a dense layer and a loose layer, the dense layer can block the invasion of soft tissues, and the porous structure of the loose layer is more conducive to the attachment and growth of bone cells.

[0003] The existing guided tissue regeneration membranes are mainly obtained th...

Claims

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