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Fluorescent latex granular immune chromatography by time resolution

A fluorescent latex particle, immunochromatography technology, applied in the direction of analysis of materials, fluorescence/phosphorescence, material excitation analysis, etc., to achieve the effect of improving sensitivity, good hydrophilicity, and fast flow rate

Inactive Publication Date: 2006-08-16
SHANGHAI JIAOTONG UNIV SCHOOL OF MEDICINE
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Problems solved by technology

The research and development of domestic POCT immunoassay reagents mostly focus on the qualitative and semi-quantitative research of gold standard immunoassay. The research and development of gold standard quantitative POCT platform is in its infancy, and there is still a blank for innovative products in fluorescence detection

Method used

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  • Fluorescent latex granular immune chromatography by time resolution
  • Fluorescent latex granular immune chromatography by time resolution

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preparation example Construction

[0012] (1) Preparation of biotinylated antibody: Monoclonal antibody was purified by octanoic acid method [Xu Zhaohua, Tao Yixun, Chen Fuping: Shanghai Journal of Immunology 1989; 9: 45]. Biotin-labeled monoclonal antibody method [Ternynck T, Avramers S: Methods in Enzymology1990; 184: 469], adding activated long-chain biotin (NHS-LC-biotin) to 1 mg / ml antibody solution at a molar ratio of 1:100 , mix well and place at room temperature for 2 hours, put into a dialysis bag and dialyze against phosphate buffer saline PBS, 0.02mol / l, pH7.2 for 48 hours, add an equal volume of glycerol, and store at -20°C for later use.

[0013] (2) Preparation of immune time-resolved fluorescent particles

[0014] Covalent coating method: polymer polystyrene particles with a diameter of 100nm, embedded with europium-chromium compound, have the characteristics of time-resolved fluorescence, and the surface of the particles has a high-density functional group-COOH, take 25mg of particles and use ME...

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Abstract

A microparticle immune chromatography of time-resolution fluorescence emulsion includes preparing biotinylation antibody and preparing immune time-resolution fluorescence microparticles, using Fusion 5 film to prepare avidin solid phase film detection line and quality control line by utilizing avidin to envelop said film, finally using double-antibody sandwich method to detect antigen quickly.

Description

Technical field [0001] The time-resolved fluorescent latex microparticle immunochromatography method of the present invention belongs to the technical field of solid-phase membrane immunoassay, and specifically relates to a preparation method and application of a solid-phase film suitable for immunochromatography tests, especially as a rapid assay method for latex microparticles. means of separation and capture. Background technique [0002] In recent years, providing reliable and instant test results is one of the development directions of laboratory medicine. This demand has prompted the continuous introduction of various medical test (POCT) products that can be performed around patients. The gold-labeled immunoassay technology developed in the early 1990s has been widely used clinically, and the detection items involved include the detection of infectious disease antigens, antibodies, tumor markers, myocardial markers, hormones and drugs. However, the early POCT detecti...

Claims

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Application Information

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IPC IPC(8): G01N33/543G01N21/64G01N33/577
Inventor 郑佐娅
Owner SHANGHAI JIAOTONG UNIV SCHOOL OF MEDICINE
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