The invention discloses an anti-TNF-alpha monoclonal antibody drug antibody detection reagent kit. The reagent kit comprises biotinylation monoclonal antibody drugs, a calibrator, a quality control product, an enzyme conjugate, magnetic particle reagent, a chemiluminiscence substrate and cleaning fluid. In this way, the full-automatic magnetic particle chemiluminiscence detection method is adopted, an alkaline phosphatase (AP)-adamantane AMPPD system is selected, the method has the advantages that stability and repeatability are good, and sensitivity is high, meanwhile, detection time is greatly shortened, one test is completed within 50 minutes, operation is easy and convenient, and detection full automation is really achieved.