Kit and method for detecting procalcitonin

Abstract

The invention discloses a kit for detecting procalcitonin. The kit comprises a luminous substrate, a magnetic particle coupling antibody, a biotinylation antibody and an enzyme marker, wherein the enzyme marker is horseradish peroxidase labeled streptavidin; and the antibody is a procalcitonin monoclonal antibody. In the kit, the magnetic particle coupling antibody is used as a fixed separation phase, a biotin-streptavidin multistage amplification system is adopted, the luminous substrate is catalyzed by using horseradish peroxidase serving as catalytic enzyme to emit light, and the detectionsensitivity and accuracy of reagents are improved.

Description

technical field [0001] The invention relates to the field of immune analysis, in particular to a kit for detecting procalcitonin and a detection method for procalcitonin. Background technique [0002] In recent years, the pathological and physiological processes of systemic infection have been better understood, and intensive supportive treatment has also been significantly improved. However, systemic infection and its complications are still the leading cause of death in non-cardiac intensive care units, the United States Approximately 500,000 patients with systemic infections are diagnosed each year, and approximately 40% eventually die. Therefore, there is a need for a marker that can more effectively reflect the degree of inflammatory damage caused by infection. Procalcitonin (abbreviated as PCT) is a new indicator with high sensitivity and specificity. It is significantly elevated when bacterial or fungal infection is combined with severe systemic reactions or organ hy...

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Problems solved by technology

[0010] The above methods for detecting PCT have the following disadvantages: 1) The luminescence efficiency of direct labeling antibodies (or antigens) with acridinium ester or isoluminol is low, and the markers are unstable. The luminescence process is completed in less than 1s), it is difficult to guarantee the repeatability of the results, and a complex and accurate in-situ detection mechanism is required, which increases the difficulty of the design of the detection instrument; 2) The reaction tube or the well of the luminescent plate is directly coated as a fixed phase, which has a limited internal surface area and cannot achieve a higher antibody coating density, which limits the improvement of sensitivity. In addition, the entire immune reaction process is approximately diffused from the liquid phase to the solid phase, resulting in a very slow immune process and prolonging the reaction time; 3 ) Electrochemiluminescent products using biotin-avidin system to detect PCT are currently imported products. This product belongs to electrode-induced luminescent reaction, electrode excitation and detection module design is complicated, and the kit adopts closed automatic electrochemical Luminescence system requires expensive fully automatic electrochemiluminescence detection equipment

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