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Process for preparing gold nano particles through reduction of chloroauric acid by catalase

A technology of catalase and tetrachloroauric acid, which is applied in the field of preparation of gold nanoparticles, can solve the problems of easy aggregation of gold nanoparticles, large environmental impact, complex process, etc., and achieve good repeatability, environmental friendliness, and easy operation steps easy effect

Active Publication Date: 2013-01-02
苏州奕方华知识产权运营有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The invention provides a method for preparing gold nanoparticles by reducing tetrachloroauric acid with catalase, which solves the problems of easy aggregation of gold nanoparticles, poor repeatability, complicated process, and large environmental impact of reagents used in the preparation in the traditional method

Method used

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  • Process for preparing gold nano particles through reduction of chloroauric acid by catalase
  • Process for preparing gold nano particles through reduction of chloroauric acid by catalase

Examples

Experimental program
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Effect test

Embodiment 1

[0028] (1) under magnetic stirring condition, the bovine liver catalase (Aladdin Reagent Co., Ltd.) aqueous solution that concentration is 5mg / mL is added dropwise in the tetrachloroauric acid aqueous solution that concentration is 1%, until catalase and The mol ratio of tetrachloroauric acid is 0.00221: 1, magnetic stirring 4h;

[0029] (2) Add 1mol / L sodium hydroxide solution to the solution obtained in (1), adjust the pH of the solution to 10, stir in a water bath at 25°C, until the UV spectrum curve of the gold nanoparticle sol generated by the reaction does not change, The obtained gold nanoparticle sol is centrifuged at a rotational speed of 8000 r / min to obtain gold nanoparticles.

[0030] from figure 1 It can be seen that the obtained material is a gold nanoparticle with a particle size between 10-20nm; figure 2 It is shown that the generated gold nanoparticles will not agglomerate under high salt conditions (0.5MNaCl).

Embodiment 2

[0032] (1) under magnetic stirring condition, be that the bovine liver catalase aqueous solution of 5mg / mL is added dropwise in the tetrachloroauric acid aqueous solution of concentration by 5mg / mL, until the mol ratio of catalase and tetrachloroauric acid 0.0221:1, magnetically stirred for 2h;

[0033] (2) Add 1mol / L sodium hydroxide solution to the solution obtained in 1), adjust the pH of the solution to 11, stir in a water bath at 30°C, until the reaction generates gold nanoparticles sol UV spectrum curve does not change, and the obtained The gold nanoparticle sol is centrifuged at a speed of 8000 r / min to obtain gold nanoparticles.

[0034] from image 3 It can be seen that the obtained material is gold nanoparticles with a particle size between 10-40 nm.

Embodiment 3

[0036] (1) under magnetic stirring condition, be that the bovine liver catalase aqueous solution of 20mg / mL is added dropwise in the tetrachloroauric acid aqueous solution of concentration by 20mg / mL, until the mol ratio of catalase and tetrachloroauric acid 0.0885:1, magnetically stirred for 60 minutes;

[0037] (2) Add 2mol / L sodium hydroxide solution to the solution obtained in (1), adjust the pH of the solution to 10, stir in a water bath at 35°C, until the reaction generates gold nanoparticles sol UV spectrum curve does not change, the The obtained gold nanoparticle sol is centrifuged at a rotational speed of 8000 r / min to obtain gold nanoparticles.

[0038] from Figure 4 It can be seen that the obtained material is gold nanoparticles with a particle size between 10-40 nm.

[0039] Figure 5 Among them, the three curves correspond to Examples 1-3 respectively, which are the visible spectrum absorption curves of gold nanoparticles prepared under different catalase / tetr...

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Abstract

The invention discloses a process for preparing gold nano particles through reduction of chloroauric acid by catalase. The process comprises the steps of dropping a catalase solution into a chloroauric acid solution, and mixing uniformly; and adjusting the pH value of a mixed solution to be alkaline, reacting on the water bath condition of 20-37 DEG C, and obtaining gold nano particles through separation after the reaction is completed. According to the process, the catalase is introduced to serve as a reducing agent and a protective agent, reducing functional groups on the catalase are high in reducibility on the alkaline condition, the synthesis of gold nano particles is facilitated, and the produced gold nano particles are free from agglomeration on the high salinity condition (0.5MNaCl).

Description

technical field [0001] The invention relates to a preparation method of gold nanoparticles, in particular to a method for preparing gold nanoparticles by reducing tetrachloroauric acid with catalase. Background technique [0002] Gold nanoparticles refer to particles with a diameter of 1-100 nm. Gold nanoparticles have special surface effects, volume effects, quantum size effects and macroscopic quantum tunneling effects, and have important applications in many fields such as catalysis, electronics and optical devices, and biotechnology. Gold nanoparticles have an absorption peak in the visible spectral range of 510-550nm, and the absorption wavelength increases with the diameter of gold nanoparticles. When the particle size increases from small to large, the apparent color changes from light orange yellow, wine color, deep red and blue purple in sequence. [0003] The surface of gold nanoparticles is atomically arranged. Due to the existence of a large number of crystal d...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B22F9/24
Inventor 包炜幸徐高峰涂陈弟奚凤娜
Owner 苏州奕方华知识产权运营有限公司
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