Atmosphere heavy metal pollution prewarning method using muskeg catalase as marking article

Abstract

The invention relates to an environmental protection technical field, in particular to a method for the early detection of the heavy metal pollution in air by taking the moss catalase as a marking object; the steps of the method are as follows: firstly mosses are collected, and the catalase is extracted from the mosses, finally the catalase is analyzed and the extent of the heavy metal pollution in air is judged according to the activity of the catalase. Because the activity of the catalase of the mosses is analyzed and the extent of the heavy metal pollution in air is judged according to the activity of the catalase, the invention has the advantages of flexibility, high efficiency, easy operation and low cost; the method has good early warning effect for the heavy metal pollution in the air, and is particularly applied to conditions in which pollutants are too low to cause morphological changes of plants.

Description

technical field

[0001] The invention relates to a method for monitoring and early warning of atmospheric heavy metal pollution in the field of environmental protection technology, in particular to a method for monitoring and early warning of atmospheric heavy metal pollution using moss catalase (CAT) as a biomarker. Background technique

[0002] Conventional atmospheric monitoring methods not only require expensive instruments and equipment, but also need to design a strict monitoring network, which is easily limited by technology and funds, and most of the monitoring indicators are sulfur dioxide, nitrogen oxides, and suspended particulate matter. The monitoring technology of atmospheric heavy metals is relatively lacking. The use of bryophytes for biological monitoring can avoid the fluctuation of analytical data brought about by the short-term determination, and the cost is relatively low, so it has great advantages. However, the current application of moss biological mo...

Examples

Embodiment 1

[0025] First, the gray moss was treated with lead chloride (PbCl 2 ) soaking treatment, take it out after 48 hours, blot the surface moisture, and store it in a -70°C refrigerator for later use. Weigh 0.5g of moss, grind and extract with 10mL 0.1M potassium phosphate buffer (pH 7.0) (K-PBS, containing 0.1mM EDTA and 1% polyvinylpyrrolidone) in an ice bath, centrifuge at 10,000rpm for 10min at 4°C, The supernatant is the primary enzyme solution.

[0026] Take 0.2mL initial enzyme solution to measure CAT activity, the reaction system is 3mL, including 100mM K-PBS (pH7.0), 10mM H 2 o 2 and 0.2mL enzyme solution (the initial enzyme night heated at 95°C for 30min was used as the control). Join H 2 o 2 Afterwards, the reaction started, and the change of absorbance after 1 min of reaction was measured at 240 nm, and the extinction coefficient was 39.4 / mM cm.

[0027] The relationship between CAT activity and lead ion concentration ( figure 1 ), the abscissa in the figure is th...

Embodiment 2

[0029] First, the gray moss was treated with nickel chloride (NiCl 2 ) soaking treatment, take it out after 48 hours, blot the surface moisture, and store it in a -70°C refrigerator for later use. Weigh 0.5g of moss, grind and extract with 10mL 0.1M potassium phosphate buffer (pH 7.0) (K-PBS, containing 0.1mM EDTA and 1% polyvinylpyrrolidone) in an ice bath, centrifuge at 10,000rpm for 10min at 4°C, The supernatant is the primary enzyme solution.

[0030] Take 0.2mL initial enzyme solution to measure CAT activity, the reaction system is 3mL, including 100mM K-PBS (pH7.0), 10mM H 2 o 2 and 0.2 mL of primary enzyme solution (the primary enzyme solution heated at 95°C for 30 minutes was used as the control). Join H 2 o 2 Afterwards, the reaction started, and the change of absorbance after 1 min of reaction was measured at 240 nm, and the extinction coefficient was 39.4 / mM cm.

[0031] The relationship between CAT activity and nickel ion concentration was obtained ( figure 2...

technical field

[0001] The invention relates to a method for monitoring and early warning of atmospheric heavy metal pollution in the field of environmental protection technology, in particular to a method for monitoring and early warning of atmospheric heavy metal pollution using moss catalase (CAT) as a biomarker. Background technique

[0002] Conventional atmospheric monitoring methods not only require expensive instruments and equipment, but also need to design a strict monitoring network, which is easily limited by technology and funds, and most of the monitoring indicators are sulfur dioxide, nitrogen oxides, and suspended particulate matter. The monitoring technology of atmospheric heavy metals is relatively lacking. The use of bryophytes for biological monitoring can avoid the fluctuation of analytical data brought about by the short-term determination, and the cost is relatively low, so it has great advantages. However, the current application of moss biological mo...

Examples

Embodiment 1

[0025] First, the gray moss was treated with lead chloride (PbCl 2 ) soaking treatment, take it out after 48 hours, blot the surface moisture, and store it in a -70°C refrigerator for later use. Weigh 0.5g of moss, grind and extract with 10mL 0.1M potassium phosphate buffer (pH 7.0) (K-PBS, containing 0.1mM EDTA and 1% polyvinylpyrrolidone) in an ice bath, centrifuge at 10,000rpm for 10min at 4°C, The supernatant is the primary enzyme solution.

[0026] Take 0.2mL initial enzyme solution to measure CAT activity, the reaction system is 3mL, including 100mM K-PBS (pH7.0), 10mM H 2 o 2 and 0.2mL enzyme solution (the initial enzyme night heated at 95°C for 30min was used as the control). Join H 2 o 2 Afterwards, the reaction started, and the change of absorbance after 1 min of reaction was measured at 240 nm, and the extinction coefficient was 39.4 / mM cm.

[0027] The relationship between CAT activity and lead ion concentration ( figure 1 ), the abscissa in the figure is th...

Embodiment 2

[0029] First, the gray moss was treated with nickel chloride (NiCl 2 ) soaking treatment, take it out after 48 hours, blot the surface moisture, and store it in a -70°C refrigerator for later use. Weigh 0.5g of moss, grind and extract with 10mL 0.1M potassium phosphate buffer (pH 7.0) (K-PBS, containing 0.1mM EDTA and 1% polyvinylpyrrolidone) in an ice bath, centrifuge at 10,000rpm for 10min at 4°C, The supernatant is the primary enzyme solution.

[0030] Take 0.2mL initial enzyme solution to measure CAT activity, the reaction system is 3mL, including 100mM K-PBS (pH7.0), 10mM H 2 o 2 and 0.2 mL of primary enzyme solution (the primary enzyme solution heated at 95°C for 30 minutes was used as the control). Join H 2 o 2 Afterwards, the reaction started, and the change of absorbance after 1 min of reaction was measured at 240 nm, and the extinction coefficient was 39.4 / mM cm.

[0031] The relationship between CAT activity and nickel ion concentration was obtained ( figure 2...

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