Simultaneous measurement of ligustrazine and aspirin in blood plasma by LC-MS

Abstract

The invention provides simultaneous measurement of ligustrazine and aspirin in blood plasma by an LC-MS, and relates to the technical field of medicines. A chromatographic column filled with octadecylbonded silica gel is included, the flow rate of a liquid phase is 0.6 ml / min to 1.4 ml / min, and a mobile phase is prepared from methanol and a formic acid aqueous solution according to a certain proportion; a liquid chromatography-mass spectrometry tandem isocratic elution method is used for separating and analyzing ligustrazine, aspirin and in-vivo metabolites of ligustrazine and aspirin after aplasma sample is treated, and the method is sensitive, efficient, low and accurate in detection concentration, simple and easy to operate, capable of effectively detecting whether a tested sample hasbioequivalence with a reference preparation or not and high in sensitivity; a single component can be detected, a corresponding atrioventricular model can be established on the basis of the detectedblood concentration, and indexes such as key parameters Cmax, Tmax, T1 / 2 and AUC of the atrioventricular model are solved, so that whether bioequivalence exists between preparations or not is deduced.

Description

technical field [0001] The invention relates to the technical field of medicine, in particular to a detection method for the simultaneous determination of ligustrazine and aspirin in blood plasma by LC-MS. Background technique [0002] At present, there are the following disadvantages: Aspirin and ligustrazine are effective drugs in the cardiovascular field, and the combination can reduce the bleeding tendency when aspirin acts alone, and can also synergistically exert the repair and protection effect on the cardiovascular and cerebrovascular, and exert the pharmacological effect of the combination of Chinese and Western medicine. In order to prove its bioequivalence with commercially available reference preparations, it is urgent to develop a method for the simultaneous determination of the corresponding two components in plasma samples, so as to fill in the poor compliance of patients who need to be administered at the same time and the lack of simultaneous detection of the...

AI Technical Summary

Problems solved by technology

[0002] At present, there are the following disadvantages: Aspirin and ligustrazine are effective drugs in the cardiovascular field, and the combination can reduce the bleeding tendency when aspirin acts alone, and can also synergistically exert the repair and protection effect on the cardiovascular and cerebrovascular, and exert the pharmacological effect of the combination of Chinese and Western medicine. In order to prove its bioequivalence with commercially available reference preparations, it is urgent to develop a method for the simultaneous determination of the corresponding two components in plasma samples, so as to fill in the poor compliance of patients who need to be administered at the same time and the lack of simultaneous detection of the two blood drugs. Concentration Method Blank

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