Analytical method of ecological effect influence of domestic-garbage-leachate-contaminated soil
A technology for polluting soil and domestic waste, applied in soil material testing, material inspection products, etc., can solve problems such as unstable composition, high toxicity, and large pollution area, and achieve the effect of simple operation and easy implementation
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example 1
[0025] A site polluted by domestic waste leachate in a certain place was selected as the analysis object. After removing the top 10cm of soil, dig out 4-5kg of soil and put it in a sealed plastic bag or bucket for later use. Soil samples were air-dried at room temperature to constant weight, crushed manually or mechanically, and then sieved through a 10-mesh sieve. The control soil samples were treated in the same way. Take 1kg of soil for each sample and put it in a glass or plastic container. Make 3 parallel samples of the object soil and the control soil. Adjust the moisture content of each sample soil to 60% of the field water holding capacity, and cultivate it at a constant temperature at 25°C for 2-3 days. Perform indicator detection. The soil microbial activity (respiration intensity and soil enzyme activity (catalase, amylase, urease and sucrase)) and biological toxicity (protozoan lethality) of soil samples were detected. The protozoan adopts Tetrahymena piriformis,...
example 2
[0031] The sampling and testing methods are the same as in Example 1, wherein the protozoan is Pseudonephron moschii, the constant temperature culture temperature is 20° C., and the mixing ratio of medium and supernatant in the mixed liquid is 1:15.
[0032] The monitoring data are as follows:
[0033]
[0034] Note: The unit of respiratory intensity is mgCO 2 / (g soil h), the unit of catalase activity is ml (0.1mol KMnO 4 ) g -1 (soil), amylase activity unit is mg (glucose) g -1 (soil), the unit of urease activity is mg (NH 3 -N)·g -1 (soil), the sucrase activity unit is ml (0.1mol Na 2 S 2 o 3 ) g -1 (soil), the unit of protozoan lethality is %.
[0035] According to the rules of the analysis provided in step 3, it is considered that the leachate pollution has caused serious adverse ecological effects.
example 3
[0037] Sampling and testing methods are the same as in Example 1, wherein the protozoan adopts dilated nephrite, the constant temperature culture temperature is 25° C., and the mixing ratio of medium and supernatant in the mixed liquid is 1:20.
[0038] The monitoring data are as follows:
[0039]
[0040] Note: The unit of respiratory intensity is mgCO 2 / (g soil h), the unit of catalase activity is ml (0.1mol KMnO 4 ) g -1 (soil), amylase activity unit is mg (glucose) g -1 (soil), the unit of urease activity is mg (NH 3 -N)·g -1 (soil), the sucrase activity unit is ml (0.1mol Na 2 S2 o 3 ) g -1 (soil), the unit of protozoan lethality is %.
[0041] Following the rules of the analysis provided in Step 3, no adverse ecological effects from leachate contamination were considered.
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