Calorimetric method for detecting pesticides by using acetylcholin esterase

A technology of acetylcholinesterase and choline oxidase, which is applied in the field of pesticide detection, can solve the problems of difficult calorimetry detection, low detection sensitivity, and large heat release, and achieve lower detection limit, high sensitivity, and improved accuracy Effect

Inactive Publication Date: 2013-07-10
QINGDAO UNIV
View PDF4 Cites 9 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to overcome the heat that acetylcholinesterase enzymatic reaction produces is less, be used for the shortcoming that calorimetry detection difficulty is big and detection sensitivity is low; Realize the high sensitivity of acetylcholinesterase to organic phosphorus and carbamate pe...

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Calorimetric method for detecting pesticides by using acetylcholin esterase
  • Calorimetric method for detecting pesticides by using acetylcholin esterase

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0025] The technical solution of the present invention will be described in detail below in conjunction with the accompanying drawings, and the content of the present invention will be illustrated by the following in conjunction with specific embodiments, rather than limiting the scope of the present invention.

[0026] use figure 1 The flow injection immobilized enzyme thermal analysis system shown is composed of constant flow pump P, first reactor R1, second reactor R2, reference reactor R3, injection valve V1, injection valve V2, thermostat T, thermal detection Composed of detector D1, thermal detector D2, signal processing and display unit C&D, and computer PC, the constant current pump P realizes the constant flow of the set flow rates of the carrying current C1, carrying current C2, sample liquid S1 and substrate S2, ensuring heavy Reality and precision; injection valve V1 realizes timing and quantitative injection of sample liquid S1 and substrate S2, and injection valv...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to a calorimetric method for detecting pesticides by using acetylcholin esterase. According to the technical scheme, an enzymatic inhibition method and cascade reaction are adopted; the acetylcholin esterase is suppressed by pesticides, so that the enzymatic reaction rate is reduced; the quantity of choline products is reduced and is in direct proportion to the suppression degree; and furthermore, the amount of the choline can be obtained by cascade reaction heat of choline oxidase and catalase. By using the cascade reaction, the intensity of an original thermal signal as well as the detection sensitivity and the detection resolution are greatly improved, the detection limit is reduced, and the detection accuracy is effectively improved; a pesticide detection calorimetric method based on the acetylcholin esterase is realized; the advantages of high sensitivity of the acetylcholin esterase to organophosphorus and carbamic acid ester type pesticides and no interference caused by electrochemical active substances or colors and turbidity of the sample on the calorimetric method are integrated; the in-site quick pesticide residue detection is facilitated; and the large-scale application is facilitated.

Description

technical field [0001] The invention relates to a calorimetric method for rapidly detecting pesticides by using acetylcholinesterase, in particular to an acetylcholinesterase calorimetric analysis method capable of realizing thermal amplification, and belongs to the field of pesticide detection. Background technique [0002] Calorimetry is analyzed by measuring the heat change in the biochemical reaction process. Compared with optical and electrochemical methods, it has strong versatility and is not interfered by the color, turbidity, and electrochemical properties of the sample itself, and can meet the requirements of actual samples. It is especially suitable for rapid on-site testing, and the detection principle is simple and reliable, which is conducive to the development of micro-systems. [0003] Acetylcholinesterase (AchE) is essential for the normal nerve conduction activities of organisms. Because organophosphate and carbamate pesticides can inhibit the normal functi...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): G01N25/48
Inventor 郑艺华刘君
Owner QINGDAO UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap