Degradation bacteria for carbendazim pesticide residue and bacterial agent produced thereby

A pesticide residue degradation and carbendazim technology, which is applied in the field of carbendazim pesticide residue degradation bacteria and the bacterial agents produced, can solve the problems of ecological system hazards, agricultural product exports that affect health, and pesticide residues exceeding the standard, etc., to achieve protection The effect of ecological environment, protection of health, and low cost of production and use

Inactive Publication Date: 2006-09-06
NANJING AGRICULTURAL UNIVERSITY
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Problems solved by technology

But at the same time, pesticides are some toxic and harmful chemicals. After they enter the ecological environment, they not only kill the organisms that are harmful to humans, but also cause serious harm to ...

Method used

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Examples

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Embodiment Construction

[0017] Examples of the present invention are described below.

[0018] Activate the original seed of the degrading bacteria DJL-6 of the present invention on a petri dish, measure its degradation performance, and inoculate it on the inclined surface of a test tube for future use. The test tube seed was inoculated in a 1000ml shake flask, and 200ml of LB medium was added to it, and the culture was shaken at a constant temperature to the logarithmic phase, ready to inoculate the seed tank. 500 liters of seed tanks, 400 liters of feeding capacity, the medium formula is: glucose 0.8%, (NH 4 ) 2 SO 4 1%, K 2 HPO 4 0.2%, MgSO 4 0.05%, NaCl 0.01%, CaCO 3 0.3%, yeast extract 0.02%, pH 7.2-7.5. After the feeding is completed, 121°C high-pressure damp heat sterilization, after cooling to 33°C, inoculate the above-mentioned cultivated shake flask strains into a 500-liter seed tank according to the inoculum amount of 10%, and cultivate to the logarithmic growth phase, and the s...

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Abstract

The invention provides a carbendazim pesticide residue degrading bacteria and a prodegradant, which belongs to biological high-tech domain. The used bacterial strain DJL-6 is judged as Rhodococcus sp. The main biological feature is: G+, the thallus is of ball rod and atrichosis; the reaction between catalase and V-P is positive; the oxidase reaction is negative; the barley sugar, carubinose, citrate, acetamide and agedoite can be used as unique carbon source or nitrogen source to carry out its growth, but the lactin and bitter almond acid can not be used as unique carbon source to carry out its growth; and the bacterial colony is orange when cultivated on LB culture medium. The pesticide residual quantity of the crop can be reduced by more than 95 % by directly applying the degrading bacteria product, which solves the overproof pesticide residue problem, and can produce non-toxic and nuisanceless environmental farm product.

Description

1. Technical field [0001] The invention discloses a carbendazim pesticide residue degrading bacterium and the bacterial agent produced therefrom, which belong to the high-tech field of biology, use microorganisms to degrade chemical pesticide residues, and are suitable for the production and processing of green and pollution-free agricultural products in modern agricultural production. 2. Technical Background [0002] The use of chemical pesticides has greatly promoted the development of modern agriculture, restored a large number of losses caused by diseases and insect pests for agricultural production, and greatly improved the labor efficiency and mechanization degree of agricultural production, playing a role in the modernization of agricultural production. immeasurable effect. But at the same time, pesticides are some toxic and harmful chemicals. After they enter the ecological environment, they not only kill the organisms that are harmful to humans, but also cause serio...

Claims

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Application Information

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IPC IPC(8): C12N1/20A62D3/00
Inventor 李顺鹏许敬亮沈标何健洪青
Owner NANJING AGRICULTURAL UNIVERSITY
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