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Preparing method and application of dual-response sandwich-type immunosensor based on TiO2 mesomorphic nanomerter material

An immunosensor and nanomaterial technology, which is applied in the field of preparation of dual-response sandwich type immunosensors to achieve the effects of high selectivity and sensitivity, improved sensitivity and stability, and high sensitivity

Inactive Publication Date: 2017-03-22
FUJIAN NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Despite the many advantages of these assays, finding a new method to improve the accuracy, selectivity, and sensitivity of IL-6 detection remains a challenge

Method used

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  • Preparing method and application of dual-response sandwich-type immunosensor based on TiO2 mesomorphic nanomerter material
  • Preparing method and application of dual-response sandwich-type immunosensor based on TiO2 mesomorphic nanomerter material
  • Preparing method and application of dual-response sandwich-type immunosensor based on TiO2 mesomorphic nanomerter material

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Experimental program
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Embodiment 1

[0038] A TiO-based 2 Preparation of dual-response sandwich-type immunosensors based on mesogenic nanomaterials (e.g. figure 1 shown):

[0039] (1) The glassy carbon electrode (GCE) is first mechanically polished and polished on the suede covered with alumina powder, washed with secondary water to remove the residual powder on the surface, and then moved into an ultrasonic water bath for cleaning until it is clean, and finally with ethanol in sequence, Wash thoroughly with dilute acid and water;

[0040] (2) Add 4 μL anatase TiO dropwise 2 Mesogens (AMCs) / Ru(bpy) 3 2+ The solution was dried on the surface of a clean glassy carbon electrode at 60°C for 20min to prepare AMCs / Ru(bpy) 3 2+ / GCE electrode;

[0041] (3) Add dropwise 4 μL carboxylated ionic liquid (CTIL) solution to AMCs / Ru(bpy) 3 2+ / GCE modified electrode surface, dried at 60°C for 20min to prepare CTIL / AMCs / Ru(bpy) 3 2+ / GCE electrode;

[0042] (4) Combine CTIL / AMCs / Ru(bpy) 3 2+ / GCE modified electro...

Embodiment 2

[0047] Preparation of the carboxylated ionic liquid (CTIL) of the above-mentioned embodiment 1:

[0048] 3.3g of methylimidazole and 5.7g of chloroacetic acid were refluxed in 20ml of toluene for 24h, recrystallized and purified to obtain carboxylated ionic liquid.

Embodiment 3

[0050] The anatase type TiO of above-mentioned embodiment 1 2 Mesogens (AMCs) / Ru(bpy) 3 2+ Solution preparation:

[0051] 4.5g of sodium dodecyl sulfate (SDS) was dissolved in 150ml of hydrochloric acid solution with a concentration of 2 mol / L, and stirred for 5 minutes; 4.5ml of titanium tetraisopropoxide (IV) was added, and stirred at 80°C for 48 h, The precipitate was collected by centrifugation and washed thoroughly with distilled water; dried at 60 °C for 12 h, and then calcined at 400 °C in air for 30 min to remove residual organic matter to obtain anatase TiO 2 Mesogen cages (AMCs). Electron emission scanning electron microscopy (SEM), transmission electron microscopy (TEM), high-resolution transmission electron microscopy (HRTEM) and selected area electron diffraction (SAED) images of AMCs, such as Figure 2A , Figure 2B , Figure 2C and Figure 2C Inset, indicating the formation of AMCs.

[0052] 500 μL of AMCs solution with a concentration of 10mg / mL and 50...

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Abstract

The invention discloses a preparing method and application of a dual-response sandwich-type immunosensor based on TiO2mesomorphic nanomerter material. The invention is characterized in that a novel dual-response sandwich-type immunosensor is prepared by combining electrochemistry and an electrochemical luminance method on the basis of two kinds of TiO2 mesomorphic nanomerter materials, and the dual-response sandwich-type immunosensor is applicable to detection of interleukin-6. Anatase type TiO2 mesocages materials and ionic liquid are used for fixing Ru(bpy)<3>2+ and an IL-6 antibody respectively, and the Ru(bpy)<3>2+ and the IL-6 antibody are used as a signal probe and a molecular recognition probe; octahedron anatase type TiO2 mesocages materials are used for fixing IL-6 second antibody and acid phosphatase which are marked by horse radish peroxidase, and the IL-6 second antibody and acid phosphatase are subjected to a typical sandwich-type immunoreaction to conduct self-assembly on surface of the electrode to prepare the IL-6 sandwich-type immunosensor. The prepared sandwich-type immunosensor can produce electrochemistry signals and electrochemical luminance signals, wherein signal values and IL-6 concentration present linear states within a range of 10<-6>-90 pg / ml and a range of 10<-8>-90 pg / ml respectively.

Description

technical field [0001] The invention belongs to the technical field of novel functional materials and biosensing detection, and specifically relates to a TiO-based 2 Preparation method and application of double-response sandwich immunosensor of mesogenic nanomaterials. Background technique [0002] Interleukin 6 (IL-6), referred to as IL-6, is involved in a large number of biologically active processes, such as the growth of hybridoma cells and the regulation of the immune system. In addition, IL-6 is a potential factor in the diagnosis of diseases, including diabetes, atherosclerosis, depression, Alzheimer's disease, cancer, etc. Various analytical methods have been applied to the detection and analysis of IL-6 in various diseases, including enzyme-linked immunosorbent assay, surface plasmon resonance, fluorescent chip, chemiluminescence immunoassay, electrochemical immunoassay and electrochemiluminescence immunoassay. Despite the many advantages of these assays, finding ...

Claims

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Application Information

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IPC IPC(8): G01N33/53G01N33/535
CPCG01N33/5302G01N33/535
Inventor 林燕语戴宏高利红
Owner FUJIAN NORMAL UNIV
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