Copper ion detection kit based on click chemistry and G tetramer and detection method of copper ion detection kit

Abstract

The invention discloses a copper ion detection kit based on click chemistry and G tetramer with HRP (Horse Radish Peroxidase) activity and a detection method of the copper ion detection kit. A G tetramer formation sequence is formed by two DNA (deoxyribonucleic acid) sequences modified with an azide group and an alkynyl group through click chemistry in a reaction system, and then ferriheme and KCl are added to form a ferriheme/G tetramer structure. The ferriheme/G tetramer has the HRP activity and can catalyze a colourless substrate TMB (tetramethylbenzidine) to form a colored substrate, and the experiment result can be judged by eyes. When quantitative analysis is performed, the final reaction solution is transferred to an elisa plate, and the OD (optical density) value of the solution under 450nm can be read by an elisa meter. The copper ion detection kit can be used for performing qualitative and quantitative analysis of concentration of Cu<2+>, the detection system is simple, the specificity is good, the detection is not affected by other substances, the operation is easy, the reaction is fast, the detection result is reliable, and the copper ion detection kit is suitable for local laboratories.

Description

technical field [0001] The invention relates to a copper ion detection kit, in particular to a copper ion detection kit based on click chemistry and G tetramer. Background technique [0002] The concentration of ions in the environment directly affects people's health, too high or too low will affect people's health. Among many ions, heavy metal ions are more harmful, so it is necessary to accurately measure their content. [0003] The traditional methods for detecting heavy metal ions mainly include graphene atomic absorption spectrometry (graphite furnace atomic absorption spectrometry, AAS) and inductive coupled plasma atomic emission spectroscopy (inductive coupled plasma atomic emission spectroscopy, ICP-AES). These two methods are accurate and reliable for the detection of heavy metal ions, but the instruments used are expensive and require experienced technicians to operate, thus limiting their wide application in grassroots laboratories. In recent years, various bi...

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Problems solved by technology

However, some colorimetric methods need to change the reaction temperature to observe the change

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