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Copper ion detection kit based on click chemistry and G tetramer and detection method of copper ion detection kit

A technology of copper ion detection and click chemistry, which is applied in material analysis by observing the influence of chemical indicators, and analysis by chemical reaction of materials, etc., can solve the problem that the sensitivity needs to be further improved, and achieves low requirements and simplification. Operation time and steps, high sensitivity effect

Active Publication Date: 2014-02-05
GUANGZHOU INST OF BIOMEDICINE & HEALTH CHINESE ACAD OF SCI
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Problems solved by technology

However, some colorimetric methods need to change the reaction temperature to observe the change of the colloidal gold solution, and the sensitivity of these methods needs to be further improved

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  • Copper ion detection kit based on click chemistry and G tetramer and detection method of copper ion detection kit
  • Copper ion detection kit based on click chemistry and G tetramer and detection method of copper ion detection kit
  • Copper ion detection kit based on click chemistry and G tetramer and detection method of copper ion detection kit

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Embodiment Construction

[0026] The copper ion detection kit and copper ion detection method based on click chemistry and G tetramer of the present invention will be illustrated below with reference to the accompanying drawings.

[0027] Such as figure 1 As shown, the principle of the copper ion detection method of the present invention is: click chemistry (click chemistry) based on copper ions means that the azide group on one molecule can be combined with the alkynyl group on another molecule on Cu + (monovalent copper ions), linked together by a cycloaddition reaction to form a triazole five-membered ring. Cu + Cu can be reduced by ascorbate 2+ (divalent copper ions) produced. The G tetramer formation sequence described in the present invention is a DNA sequence rich in G bases. In the reaction system, click chemistry based on copper ions is used to make two DNA sequences modified with azide groups and alkynyl groups A G tetramer forming sequence is formed. Specifically, the present invention ...

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Abstract

The invention discloses a copper ion detection kit based on click chemistry and G tetramer with HRP (Horse Radish Peroxidase) activity and a detection method of the copper ion detection kit. A G tetramer formation sequence is formed by two DNA (deoxyribonucleic acid) sequences modified with an azide group and an alkynyl group through click chemistry in a reaction system, and then ferriheme and KCl are added to form a ferriheme / G tetramer structure. The ferriheme / G tetramer has the HRP activity and can catalyze a colourless substrate TMB (tetramethylbenzidine) to form a colored substrate, and the experiment result can be judged by eyes. When quantitative analysis is performed, the final reaction solution is transferred to an elisa plate, and the OD (optical density) value of the solution under 450nm can be read by an elisa meter. The copper ion detection kit can be used for performing qualitative and quantitative analysis of concentration of Cu<2+>, the detection system is simple, the specificity is good, the detection is not affected by other substances, the operation is easy, the reaction is fast, the detection result is reliable, and the copper ion detection kit is suitable for local laboratories.

Description

technical field [0001] The invention relates to a copper ion detection kit, in particular to a copper ion detection kit based on click chemistry and G tetramer. Background technique [0002] The concentration of ions in the environment directly affects people's health, too high or too low will affect people's health. Among many ions, heavy metal ions are more harmful, so it is necessary to accurately measure their content. [0003] The traditional methods for detecting heavy metal ions mainly include graphene atomic absorption spectrometry (graphite furnace atomic absorption spectrometry, AAS) and inductive coupled plasma atomic emission spectroscopy (inductive coupled plasma atomic emission spectroscopy, ICP-AES). These two methods are accurate and reliable for the detection of heavy metal ions, but the instruments used are expensive and require experienced technicians to operate, thus limiting their wide application in grassroots laboratories. In recent years, various bi...

Claims

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Application Information

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IPC IPC(8): G01N21/78
Inventor 曾令文葛晨晨赵世明王斗
Owner GUANGZHOU INST OF BIOMEDICINE & HEALTH CHINESE ACAD OF SCI
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