Copper ion detection kit based on click chemistry and G tetramer and detection method of copper ion detection kit
A technology of copper ion detection and click chemistry, which is applied in material analysis by observing the influence of chemical indicators, and analysis by chemical reaction of materials, etc., can solve the problem that the sensitivity needs to be further improved, and achieves low requirements and simplification. Operation time and steps, high sensitivity effect
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[0026] The copper ion detection kit and copper ion detection method based on click chemistry and G tetramer of the present invention will be illustrated below with reference to the accompanying drawings.
[0027] Such as figure 1 As shown, the principle of the copper ion detection method of the present invention is: click chemistry (click chemistry) based on copper ions means that the azide group on one molecule can be combined with the alkynyl group on another molecule on Cu + (monovalent copper ions), linked together by a cycloaddition reaction to form a triazole five-membered ring. Cu + Cu can be reduced by ascorbate 2+ (divalent copper ions) produced. The G tetramer formation sequence described in the present invention is a DNA sequence rich in G bases. In the reaction system, click chemistry based on copper ions is used to make two DNA sequences modified with azide groups and alkynyl groups A G tetramer forming sequence is formed. Specifically, the present invention ...
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