Graphite alkyne and click chemistry-based copper ion detection method, kit and application

A detection method and click chemistry technology, applied in the field of detection, can solve the problems of reducing the repeatability of the reaction, increasing the complexity of the reaction, etc., and achieve the effects of low detection limit, high specificity, and simplified operation time and steps.
CN110907421AActive Publication Date: 2020-03-24SHENZHEN PEOPLES HOSPITAL
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
SHENZHEN PEOPLES HOSPITAL
Publication Date
2020-03-24

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Abstract

The invention provides a graphdiyne and click chemistry-based copper ion detection method, a kit and an application. The detection method comprises the following steps of (1) adding a copper ion standard solution with a concentration gradient and a reducing agent into a mixed solution of graphdiyne and group-modified double-stranded DNA, mixing and reacting to obtain a reaction solution, testing the fluorescence intensity of the reaction solution by a fluorescence spectrometry, and drawing a standard curve according to the relationship between the concentration and the fluorescence intensity;(2) adding a to-be-detected sample solution and the reducing agent into the mixed solution of graphdiyne and the group-modified double-stranded DNA, mixing and reacting to obtain the reaction solution, testing the fluorescence intensity of the reaction solution according to fluorescence spectrometry, and quantifying the concentration of the copper ions in the to-be-detected sample according to thestandard curve obtained in the step (1). By selecting the graphite alkyne in a detection system, the situation that the alkynyl groups are used for modification is avoided, the detection efficiency can be greatly improved, and the operation steps are simplified.
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Description

technical field

[0001] The invention belongs to the field of detection, and relates to a detection method, kit and application of copper ions based on graphyne and click chemistry. Background technique

[0002] Cu 2+ It is an essential element necessary for the ligands of a large number of oxidoreductases and metalloproteins in the metabolic process of human life. These enzymes and proteins, such as tyrosinase, cytochrome c oxidase, and ceruloplasmin, play important roles in electron transfer, oxidation, and reduction. Therefore, Cu in biological systems 2+ content should be strictly controlled. Unusual levels of free Cu 2+ May act as a catalyst for the generation of oxygen and protein free radicals, leading to severe neurological diseases such as Alzheimer's, Parkinson's and Wilson's diseases. Early diagnosis is of great significance to control the occurrence and development of these diseases. For example, Cu in urine 2+ The high level is one of the clinical and diag...

Claims

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