Hydrogen peroxide and glucose sensor based on hydrophilic up-conversion nano NaYF4

A hydrophilic, sensor technology, applied in nanotechnology for sensing, nanotechnology for materials and surface science, nanotechnology, etc., can solve problems such as poor water solubility, short fluorescence lifetime, and poor stability

Inactive Publication Date: 2015-06-24
BEIJING UNIV OF CHEM TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, most organic fluorescent probes have poor water solubility, short fluorescence lif...

Method used

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  • Hydrogen peroxide and glucose sensor based on hydrophilic up-conversion nano NaYF4
  • Hydrogen peroxide and glucose sensor based on hydrophilic up-conversion nano NaYF4
  • Hydrogen peroxide and glucose sensor based on hydrophilic up-conversion nano NaYF4

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] a. Add 4.0 mg of sodium hydroxide to 10 mL of deionized water, and ultrasonically promote its complete dissolution;

[0036] b. Add 200 μL of oleylamine-modified polysuccinimide polymer solution with a concentration of 0.365 mg / mL and 200 μL of rare earth up-conversion nanoparticle dispersion with a concentration of 0.25 mmol / mL in a 1.5 mL centrifuge tube, and mix the mixture with chloroform. The solution was fixed to 1 mL; the oleylamine-modified polysuccinimide polymer solution and the rare earth up-conversion nanoparticle dispersion both used chloroform as a solvent;

[0037] c. Add the mixed solution obtained in step b to the solution prepared in step a, and use an ultrasonic crushing instrument to sonicate for 6 minutes under an ice-water bath to finally obtain an oil-in-water microemulsion with an ultrasonic power of 200W; then under the condition of 300rpm magnetic stirring , the chloroform in the solution was volatilized at normal pressure in a water bath a...

Embodiment 2

[0052] a. Add 5.0 mg of sodium hydroxide to 12 mL of deionized water, and ultrasonically promote its complete dissolution;

[0053] b. Add 240 μL of oleylamine-modified polysuccinimide polymer solution with a concentration of 0.40 mg / mL and 240 μL of rare earth up-conversion nanoparticle dispersion with a concentration of 0.25 mmol / mL in a 1.5 mL centrifuge tube, and mix the mixture with chloroform. The solution was fixed to 1.2 mL; the oleylamine-modified polysuccinimide polymer solution and the rare earth up-conversion nanoparticle dispersion both used chloroform as a solvent;

[0054] c. Add the mixed solution obtained in step b to the solution prepared in step a, and use an ultrasonic crushing instrument to sonicate for 6 minutes under an ice-water bath to finally obtain an oil-in-water microemulsion with an ultrasonic power of 260W; then under the condition of 300rpm magnetic stirring , the chloroform in the solution was volatilized at normal pressure in a water bath ...

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Abstract

The invention discloses a preparation method for a sensor based on hydrophilic up-conversion nano-particles and application of the sensor to detection of hydrogen peroxide and glucose. Monodisperse up-conversion nano-particles which are good in water solubility, stable and uniform in size are prepared under an amphiphilic system by adopting an ultrasonic micro emulsion process, utilizing hydrophobic-hydrophobic effects between oleylamine-modified polysuccinimide high polymers and oleic acid on the surfaces of the up-conversion nano-particles and hydrolyzing part of chain elements of the polysuccinimide high polymers under an alkaline condition to form a hydrophilic group; the up-conversion nano-particles are mixed with tetramethyl benzidine and horse radish peroxidase to obtain the hydrogen peroxide and glucose sensor. Compared with a conventional detecting method, the method is small in background interference, strong in signal and low in cost, and has the characteristics of being quick, accurate, high in flexibility and high in selectivity. The hydrogen peroxide and glucose sensor can be used for providing a novel opportunity for monitoring hydrogen peroxide and glucose in real time in a living microenvironment in future.

Description

technical field [0001] The invention belongs to the technical field of hydrogen peroxide and glucose sensor preparation and detection, in particular to a hydrophilic up-conversion nano-NaYF 4 Preparation of hydrogen peroxide and glucose sensor and its detection method. technical background [0002] Recent studies have shown that hydrogen peroxide produced by mammalian cells plays an important role in many physiological responses, such as cell proliferation, differentiation and migration. Therefore, the concentration of hydrogen peroxide in a living body can be regarded as one of the important parameters for diagnosing different states of a certain disease (Parkinson's disease, cancer and Alzheimer's disease). [0003] As an important physiologically active substance, glucose plays a key role in almost all processes in living cells, and an appropriate blood sugar concentration will have a beneficial effect on these processes. So glucose levels in body fluids are often used ...

Claims

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Application Information

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IPC IPC(8): G01N21/64B82Y30/00B82Y15/00
Inventor 汪乐余刘家利
Owner BEIJING UNIV OF CHEM TECH
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