Method for applying magnetic gamma-Fe2O3 nano-particle mimetic enzyme to biological detection

A nanoparticle and biodetection technology, applied in the field of nanomaterials and biomedical nanotechnology, can solve the problems of reduced enzyme activity and limited application, and achieve the effects of improving enzyme activity, increasing stability and biocompatibility
CN101672771AInactive Publication Date: 2010-03-17SOUTHEAST UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
SOUTHEAST UNIV
Publication Date
2010-03-17
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention relates to a magnetic gamma-Fe2O3 nano-particle mimetic enzyme and provides a method for applying a magnetic gamma-Fe2O3 nano-particle mimetic enzyme to biological detection. The magnetic gamma-Fe2O3 nano-particle mimetic enzyme replaces a horse radish peroxidase (HRP) to be used in biological detection. The method comprises the following steps: coupling carboxyl groups on the surface of magnetic gamma-Fe2O3 nano-particles and a specific molecular probe to construct a specific nano probe; combining the nano probe with the specificity of the corresponding target molecules to be detected; developing by using a developer containing peroxides and hydrogen-supply zymolytes; and measuring the absorbency or carrying out the micro-observation so as to realize the qualitative and quantitative detection on target molecules.
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Description

technical field

[0001] The present invention relates to magnetic gamma-Fe 2 o 3 The nanoparticle simulates enzyme and provides a method for its application in biological detection, belonging to the fields of nanomaterials and biomedical nanotechnology. Background technique

[0002] Magnetic iron oxide nanoparticles (Fe 3 o 4 , γ-Fe 2 o 3 ) because of its rich magnetic properties and good biocompatibility, it is widely used in biomedical fields such as magnetic resonance imaging contrast agents, magnetic targeting drug carriers, separation of cells and biomolecules, biosensing and detection, and magnetic induction tumor hyperthermia. Wide range of applications. In addition, in terms of industrial wastewater treatment, magnetic iron oxide nanoparticles are used for the removal of toxic substances such as phenol due to their high activity of hydroxylation of organic substances (the toxicity of organic substances such as phenol is reduced after hydroxylation). In recent y...

Claims

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