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A paraffin section method for effectively observing the anatomical structure of oil palm pistil

A technology for paraffin sectioning and anatomical structure, which is applied in the field of paraffin sectioning of plant tissue, effectively observing the anatomical structure of oil palm pistil, and can solve the problem that the structure of the film is not complete, flexible enough, and the tissue structure of oil palm pistil is easy to brown, etc. problems, to achieve the effect of clear microscopic results, clear results, and uniform thickness

Active Publication Date: 2018-11-09
COCONUT RES INST OF CHINESE ACAD OF TROPICAL AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] As the main method of plant tissue sectioning, although the production cycle of frozen section is short, the tissue structure of oil palm pistil is easy to brown and dense, and the structure of the slices cut out by frozen section is not easy to complete, and the cut slices cannot be preserved for a long time. During the cryosection process, fresh samples need to be taken for fixation and sectioning each time, which is very limited by sampling, not flexible enough, and not suitable for the observation of oil palm pistils
[0005] It is an important issue to explore the appropriate paraffin section technology for the anatomical structure and cytology of oil palm tissues. Although there have been reports on paraffin sections of other plant tissues before, different types of plant tissues have different characteristics. Reference significance, but can not be fully applied to the study of oil palm tissue slices
[0006] At present, there is no report on the application of paraffin section technology to the anatomical structure of oil palm pistils

Method used

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  • A paraffin section method for effectively observing the anatomical structure of oil palm pistil
  • A paraffin section method for effectively observing the anatomical structure of oil palm pistil
  • A paraffin section method for effectively observing the anatomical structure of oil palm pistil

Examples

Experimental program
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Effect test

Embodiment 1

[0025] 1. Sampling and fixation: First, pick the entire female inflorescence of the oil palm to be observed, and take it back to the laboratory for selection and segmentation immediately. Select fresh, normal, and representative pistils, and completely peel off the pistils near the receptacle, and then separate the sepals. Peel it off, and immediately separate the stigma from the ovary with a knife, and immediately put it into a tall weighing bottle (40×70mm) filled with Carnot’s fixative solution (pure ethanol:acetic acid=3:1), the number of samples per bottle 6 pieces, the mass-volume ratio of the material to the fixative is 1:17 (g / ml), and then a plastic film with 10 holes pierced with a toothpick is wrapped on the bottle mouth with a rubber band, and placed in a vacuum desiccator (vacuum pump model DP -01), pump for 1.2 hours. The range of the mass volume ratio of the materials in the following steps to the soaking solution is 1:17 (g / ml).

[0026] 2. Preservation and fi...

Embodiment 2

[0038] 1. Sampling and fixation: First, pick the entire female inflorescence of the oil palm to be observed, and take it back to the laboratory for selection and segmentation immediately. Select fresh, normal, and representative pistils, and completely peel off the pistils near the receptacle, and then separate the sepals. Peel it off, and immediately separate the stigma from the ovary with a knife, and immediately put it into a tall weighing bottle (40×70mm) filled with Carnot’s fixative solution (pure ethanol:acetic acid=3:1), the number of samples per bottle 6 pieces, the mass-volume ratio of the material to the fixative is 1:20 (g / ml), and then a plastic film with 12 holes pierced with a toothpick is wrapped on the bottle mouth with a rubber band, and placed in a vacuum desiccator (vacuum pump model DP -01), pump for 1.2 hours. The range of the mass volume ratio of the materials in the following steps to the soaking solution is 1:20 (g / ml).

[0039] 2. Preservation and fi...

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Abstract

The invention discloses a paraffin slicing method used for effectively observing an anatomical structure of pistil of elaeis guineensis jacq. The method comprises the steps of sampling and fixing a sample, preserving, dyeing, washing with water to back to blue, dehydrating, clearing, adding broken paraffin, infiltrating paraffin, embedding, trimming a paraffin block, cutting to form a paraffin section, spreading the paraffin section, adhering the paraffin section, drying the paraffin section, sealing the paraffin section, drying the paraffin section, observing the paraffin section and the like. The paraffin section technique is adopted to effectively obtain the paraffin section with the complete structure of pistil of elaeis guineensis jacq, the sample is not affected by brown stains after being embedded by the paraffin and can be preserved for a long period of time, the time for making the paraffin section is random, the obtained paraffin section is complete, is even in thickness and has no wrinkles, the microexamination result is clear, the method is favorable for conducting observation and researches on the anatomical structure of germplasm resources of elaeis guineensis jacq, and is of great significance in the work of cultivating the variety suitable for the domestic country and screening excellent types of germplasm resources.

Description

technical field [0001] The invention relates to the technical field of microscopic observation of plant samples, in particular to a paraffin sectioning method for plant tissue, in particular to a paraffin sectioning method for effectively observing the anatomical structure of oil palm pistils. Background technique [0002] Oil palm (Elaeis guineensis Jacq.) is a perennial woody oil crop belonging to the genus Oil Palm in the family Palmaceae. Oil palm is widely used. The main products are palm oil and palm kernel oil, which are used in food, chemical industry and bio-energy. [0003] The reproductive mode of oil palm in nature is sexual reproduction, and flowers are its important reproductive organs. The flowers of oil palm are monoecious. The pistil includes stigma, style and ovary. After fertilization, the ovary develops into fruit, and the ovules in the ovary develop into seeds. As one of the important basis for identification of germplasm resources, the study of pistil...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N1/28G01N1/06
CPCG01N1/06G01N1/2806
Inventor 石鹏李东霞曹红星雷新涛王永
Owner COCONUT RES INST OF CHINESE ACAD OF TROPICAL AGRI SCI
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