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Cigarette filter stick additive and preparation method and application thereof

A technology of cigarette filter rods and additives, applied in applications, e-liquid filter elements, tobacco, etc., can solve problems such as restrictions on wide application, and achieve the effect of mature technical means, high safety, and simple technical means

Active Publication Date: 2013-01-09
HONGYUN HONGHE TOBACCO (GRP) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to people's increasing concern about the safety of cigarette smoking and stricter restrictions on the addition of exogenous substances in cigarettes, the public tends to have doubts about the safety of adding DNA derived from salmon sperm to cigarette filters, so this project has been limited. wide application of technology

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] (1) Extraction of tobacco DNA:

[0044] a. Take 8.0~10.0g of fresh tobacco leaf sample and dry it with liquid nitrogen and quickly grind it into fine powder, transfer the obtained powder to a 50mL centrifuge tube, and add 25mL of 65℃ preheated CTAB[hexadecyltrimethylammonium bromide ( CTAB) 4g, NaCl 16.364g, 1mol / L Tris-HCl 20mL(pH8.0), 0.5mol / L EDTA 8mL, first dissolve in 70mL pure water, then dilute to 200mL, use after sterilization], shake and mix well , So that the material is completely dispersed in the extraction buffer;

[0045] b. Water bath at 65°C for 40 minutes, and mix well every 8 minutes. After the water bath, add an equal volume of chloroform / isoamyl alcohol (24:1) mixture to each tube of sample, mix well, centrifuge at 12000r / min for 10 minutes, and remove the supernatant. Transfer the solution to a new 50 mL centrifuge tube, and repeat this step once;

[0046] c. After centrifugation, transfer the supernatant to a new 50 mL centrifuge tube, add 70% volume of...

Embodiment 2

[0063] (1) Extraction of tobacco DNA:

[0064] a. Take 5.0~6.0g of fresh tobacco stem bark as the extraction material, add a little quartz sand before grinding, use liquid nitrogen to dry the stem bark and quickly grind it into a fine powder, transfer the obtained powder to a 50mL centrifuge tube, and add 20mL65 Preheated CTAB extraction buffer and 25-40μL mercaptoethanol (0.2%V / V) at ℃, shake and mix, so that the material is completely dispersed in the extraction buffer;

[0065] b. Water bath at 65°C for 1 hour, mix well every 10 minutes, add an equal volume of chloroform / isoamyl alcohol (24:1) mixture to each tube of sample after the water bath, mix well, centrifuge at 12000r / min for 10 minutes, and remove the supernatant Transfer the solution to a new 50 mL centrifuge tube, and repeat this step once;

[0066] c. After centrifugation, transfer the supernatant to a new 50 mL centrifuge tube, add 70% volume of pre-chilled isopropanol in a refrigerator at 4°C, gently mix the liquid,...

Embodiment 3

[0083] (1) Extraction of tobacco DNA

[0084] a. Take 8.0~10g of fresh tobacco anthers as the extraction material, dry it with liquid nitrogen and quickly grind it into fine powder, transfer the obtained powder to a 50mL centrifuge tube, add 20~25mL of 65℃ preheated CTAB extraction buffer and 40~ 50μL mercaptoethanol (0.2%V / V), shake and mix to make the material completely dispersed in the extraction buffer;

[0085] b. Water bath at 65°C for 45 minutes, mixing every 5 minutes. After the water bath, add an equal volume of chloroform / isoamyl alcohol (24:1) mixture to each tube of sample, mix well, centrifuge at 12000r / min for 10 minutes, and remove the supernatant. Transfer the solution to a new 50 mL centrifuge tube, and repeat this step once;

[0086] c. After centrifugation, transfer the supernatant to a new 50 mL centrifuge tube, add 70% volume of pre-chilled isopropanol in a refrigerator at 4°C, gently mix the liquid, and place it in a refrigerator at -20°C for 1 hour;

[0087] d...

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Abstract

The invention discloses a cigarette filter stick additive for reducing smoke polycyclic aromatic hydrocarbon and a preparation method and application thereof and belongs to the technical field of cigarette additives. Tobacco fresh tissue is used as raw materials, tobacco deoxyribonucleic acid (DNA) is extracted, the extracted DNA is dissolved in water, and the water solution is added in a section of a binary composite filter stick close to tobacco shred so as to enable the filter stick to hold back the polycyclic aromatic hydrocarbon in smoke. Through detection, content of the polycyclic aromatic hydrocarbon in the smoke of cigarettes adopting the cigarette filter stick additive can be reduced effectively, effect for reducing the smoke polycyclic aromatic hydrocarbon is improved along with increasing of addition amount of the cigarette filter stick additive, and the content of the polycyclic aromatic hydrocarbon in the smoke can be reduced by 25% when 2g DNA is added in each kilogram of filter sticks. Through sensory evaluation suction, cigarettes adopting the cigarette filter stick additive have no foreign smell during suction, and suction taste effect of the cigarettes is not influenced. The tobacco is used as extraction raw materials, and the cigarette filter stick additive is natural and safe, can be accepted by consumers easily, and has high application value.

Description

Technical field [0001] The invention belongs to the technical field of cigarette additives. Specifically, the invention relates to a cigarette filter additive containing tobacco DNA. At the same time, the present invention also relates to the preparation method of the additive and its application in the tobacco industry. Background technique [0002] Cigarette smoke contains more than 5,000 compounds, including a large number of aroma components and traces of harmful substances. Among these trace amounts of hazardous substances, polycyclic aromatic hydrocarbons (PAHs) are considered to be one of the main hazardous substances. Currently, there are more than one hundred types of polycyclic aromatic hydrocarbons identified, and about 30 are carcinogenic. Typical examples are 3,4-benzo[α]pyrene (BaP), benzanthracene (BaA) and so on. [0003] In order to reduce the PAHs in cigarette smoke, in the past few decades, people have developed nearly 100 kinds of filters. The commonly used p...

Claims

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Application Information

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IPC IPC(8): A24B15/24A24D3/14
Inventor 周博杨莹张天栋赵英良付磊王欣林王坚汤丹瑜陈绍田
Owner HONGYUN HONGHE TOBACCO (GRP) CO LTD
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