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Stationary liquid for preserving pathological tissues

A fixative and tissue technology, applied in the preservation, application, animal husbandry and other directions of human or animal bodies, to achieve the effect of reducing impact, improving detection effect, preservation and fixation stability

Inactive Publication Date: 2016-04-06
ZHEJIANG JIANKANG BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] From the operating room to pathology / laboratory surgery, tissue biopsy, histopathology department, gastroscopy department, etc. need to fix, store and retain samples of tissues or cells, the traditional tissue fixative uses formalin and fixes with formaldehyde Older tissues are more prone to pigmentation

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] A kind of pathological tissue preserves fixative, comprises according to the raw material of weight part: Formalin: 80ml, Na 2 HPO 4 12H 2 O: 11g, NaH 2 PO 4 2H 2 O: 2g, sodium chloride: 8g, acetic acid: 25ml, ethanol: 45ml; the above components are mixed to form a solution, which is used as a product to preserve the fixative, and the collected pathological specimens and fixative are placed in containers for preservation, Fixed pathological specimens.

[0015] When using the pathological tissue preservation fixative, select the fixative specification and capacity according to the size of the tissue, tear off the seal of the fixative reagent, open the ziplock bag or open the specimen storage bottle, put the isolated tissue within 5 minutes, and the fixative and The tissue sample volume ratio is not less than 2 times, the fixative solution is completely immersed in the tissue sample, the ziplock bag is tightly sealed, and the fixation time is 2 hours.

Embodiment 2

[0017] A kind of pathological tissue preserves fixative, comprises according to the raw material of weight part: Formalin: 90ml, Na 2 HPO 4 12H 2 O: 12g, NaH 2 PO 4 2H 2 O: 2.1g, sodium chloride: 8.5g, acetic acid: 28ml, ethanol: 48ml; the above components are mixed to form a solution, which is used as a product to store the fixative, and the collected pathological specimens and fixative are placed in containers for Preserve and fix pathological specimens.

[0018] When using the pathological tissue preservation fixative, select the fixative specification and capacity according to the size of the tissue, tear off the seal of the fixative reagent, open the ziplock bag (or open the specimen storage bottle), put the isolated tissue within 5 minutes, and fix it. The volume ratio of the solution to the tissue sample is not less than 2 times, the fixative solution is completely immersed in the tissue sample, and the ziplock bag is tightly sealed, and the fixation time is 5 hour...

Embodiment 3

[0020] A kind of pathological tissue preserves fixative, comprises according to the raw material of weight part: Formalin: 100ml, Na 2 HPO 4 12H 2 O: 13g, NaH 2 PO 4 2H 2 O: 2.14g, sodium chloride: 9g, acetic acid: 30ml, ethanol: 50ml; the above components are mixed to form a solution, which is used as a product to store the fixative, and the collected pathological specimens and fixative are placed in containers for preservation , Fixed pathological specimens.

[0021] When using the pathological tissue preservation fixative, select the fixative specification and capacity according to the size of the tissue, tear off the seal of the fixative reagent, open the ziplock bag (or open the specimen storage bottle), put the isolated tissue within 5 minutes, and fix it. The volume ratio of the solution to the tissue sample is not less than 2 times, the fixative solution is completely immersed in the tissue sample, and the ziplock bag is tightly sealed, and the fixation time is 7 ...

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PUM

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Abstract

The invention discloses stationary liquid for preserving pathological tissues. The stationary liquid is prepared from, by weight, formalin, Na<2>HPO<4>.12H<2>O, NH<2>PO<4>.2H<2>O, sodium chloride, acetic acid and ethyl alcohol; the mentioned constituents are mixed into a solution to serve as the stationary liquid for preserving a product, and a collected pathological specimen and the stationary liquid are both contained in a container, so that the pathological specimen can be preserved and fixed. By means of the stationary liquid, pathological tissue preservation and fixation can be achieved by one step, the preservation and fixation effect of the pathological tissues is remarkably improved, influence on active substances is reduced, the problem that the medical worker needs to compound stationary liquid temporarily is solved, convenience and rapidness are achieved, compounding is not required, and the stationary liquid can be used just after the container is opened; when the stationary liquid is used for preserving the pathological tissues, the specimen with the fresh tissues, in particular to specimen tissues rich in fibers, can be expanded and fix chromatin rapidly, biological enzyme is not closed, and fixation for section staining is facilitated; the neutral environment of body fluid is simulated, the pathological tissues which are preserved for a long time remain fresh, and the color is not changed. Preservation and fixation of the pathological tissues can be more stable, and it is helpful to improve the pathological section detection effect.

Description

technical field [0001] The invention relates to the technical field of pathological tissue preservation, in particular to a pathological tissue preservation fixative. Background technique [0002] From the operating room to pathology / laboratory surgery, tissue biopsy, histopathology department, gastroscopy department, etc. need to fix, store and retain samples of tissues or cells, the traditional tissue fixative uses formalin and fixes with formaldehyde Older tissues are more prone to pigmentation. Contents of the invention [0003] The purpose of the present invention is to provide a pathological tissue preservation fixative that is ready-to-use and has a good fixation effect, so as to solve the problems raised in the above-mentioned background technology. [0004] To achieve the above object, the present invention provides the following technical solutions: [0005] A pathological tissue preservation fixative, the raw materials according to parts by weight include: for...

Claims

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Application Information

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IPC IPC(8): A01N1/02
CPCA01N1/0226
Inventor 李志华段年丰唱明月
Owner ZHEJIANG JIANKANG BIOTECH CO LTD
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